Experiment: KB with Lupeol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1825 and Psyr_1826 are separated by 61 nucleotides Psyr_1826 and Psyr_1827 are separated by 137 nucleotides Psyr_1827 and Psyr_1828 are separated by 89 nucleotides Psyr_1828 and Psyr_1829 are separated by 74 nucleotides Psyr_1829 and Psyr_1830 are separated by 173 nucleotides
Psyr_1825: Psyr_1825 - DNA polymerase III, tau subunit, at 2,105,845 to 2,108,055
_1825
Psyr_1826: Psyr_1826 - Conserved hypothetical protein 103, at 2,108,117 to 2,108,443
_1826
Psyr_1827: Psyr_1827 - SirA-like protein, at 2,108,581 to 2,108,835
_1827
Psyr_1828: Psyr_1828 - 4-phosphoerythronate dehydrogenase, at 2,108,925 to 2,110,067
_1828
Psyr_1829: Psyr_1829 - conserved hypothetical protein, at 2,110,142 to 2,110,426
_1829
Psyr_1830: Psyr_1830 - ABC transporter, transmembrane region:ABC transporter, at 2,110,600 to 2,112,369
_1830
Position (kb)
2108
2109
2110
2111 Strain fitness (log2 ratio)
-1
0
1
2 at 2108.111 kb on + strand at 2108.112 kb on - strand at 2110.379 kb on + strand, within Psyr_1829 at 2110.379 kb on + strand, within Psyr_1829 at 2110.379 kb on + strand, within Psyr_1829 at 2110.380 kb on - strand, within Psyr_1829 at 2110.380 kb on - strand, within Psyr_1829 at 2110.417 kb on + strand at 2110.417 kb on + strand at 2110.417 kb on + strand at 2110.417 kb on + strand at 2110.418 kb on - strand at 2110.445 kb on - strand at 2110.457 kb on + strand at 2110.458 kb on - strand at 2110.458 kb on - strand at 2110.460 kb on - strand at 2110.552 kb on - strand at 2110.552 kb on - strand at 2110.552 kb on - strand at 2110.552 kb on - strand at 2110.858 kb on + strand, within Psyr_1830 at 2110.945 kb on + strand, within Psyr_1830 at 2110.946 kb on - strand, within Psyr_1830
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Lupeol 10 mM remove 2,108,111 + +1.0 2,108,112 - +2.3 2,110,379 + Psyr_1829 0.83 +1.3 2,110,379 + Psyr_1829 0.83 +0.4 2,110,379 + Psyr_1829 0.83 +1.1 2,110,380 - Psyr_1829 0.84 -1.0 2,110,380 - Psyr_1829 0.84 +0.9 2,110,417 + -0.6 2,110,417 + -0.1 2,110,417 + +0.6 2,110,417 + -1.5 2,110,418 - -1.0 2,110,445 - -0.3 2,110,457 + +0.6 2,110,458 - +1.1 2,110,458 - +2.3 2,110,460 - +0.1 2,110,552 - -1.3 2,110,552 - -1.0 2,110,552 - -0.8 2,110,552 - +0.7 2,110,858 + Psyr_1830 0.15 +0.1 2,110,945 + Psyr_1830 0.19 -0.3 2,110,946 - Psyr_1830 0.20 +0.1
Or see this region's nucleotide sequence