Experiment: KB with Nonanoic 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3678 and Psyr_3679 overlap by 4 nucleotides Psyr_3679 and Psyr_3680 overlap by 4 nucleotides Psyr_3680 and Psyr_3681 are separated by 25 nucleotides
Psyr_3678: Psyr_3678 - adenosylcobinamide-phosphate synthase, at 4,364,433 to 4,365,341
_3678
Psyr_3679: Psyr_3679 - cob(II)yrinic acid a,c-diamide reductase, at 4,365,338 to 4,365,988
_3679
Psyr_3680: Psyr_3680 - hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing) / cobyrinate a,c-diamide synthase, at 4,365,985 to 4,367,280
_3680
Psyr_3681: Psyr_3681 - cob(I)yrinic acid a,c-diamide adenosyltransferase, at 4,367,306 to 4,367,917
_3681
Position (kb) 4365
4366
4367
4368 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 4365.088 kb on + strand, within Psyr_3678 at 4365.089 kb on - strand, within Psyr_3678 at 4365.767 kb on + strand, within Psyr_3679 at 4365.767 kb on + strand, within Psyr_3679 at 4365.767 kb on + strand, within Psyr_3679 at 4366.043 kb on + strand at 4366.043 kb on + strand at 4366.154 kb on + strand, within Psyr_3680 at 4366.154 kb on + strand, within Psyr_3680 at 4366.301 kb on + strand, within Psyr_3680 at 4366.344 kb on + strand, within Psyr_3680 at 4366.427 kb on + strand, within Psyr_3680 at 4366.523 kb on + strand, within Psyr_3680 at 4367.429 kb on + strand, within Psyr_3681 at 4367.429 kb on + strand, within Psyr_3681 at 4367.429 kb on + strand, within Psyr_3681 at 4367.429 kb on + strand, within Psyr_3681 at 4367.430 kb on - strand, within Psyr_3681 at 4367.430 kb on - strand, within Psyr_3681 at 4367.430 kb on - strand, within Psyr_3681 at 4368.116 kb on + strand at 4368.117 kb on - strand at 4368.117 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nonanoic 10 mM remove 4,365,088 + Psyr_3678 0.72 -1.3 4,365,089 - Psyr_3678 0.72 +0.6 4,365,767 + Psyr_3679 0.66 -0.4 4,365,767 + Psyr_3679 0.66 -0.9 4,365,767 + Psyr_3679 0.66 -0.0 4,366,043 + -2.6 4,366,043 + +0.4 4,366,154 + Psyr_3680 0.13 +1.1 4,366,154 + Psyr_3680 0.13 +1.1 4,366,301 + Psyr_3680 0.24 -1.0 4,366,344 + Psyr_3680 0.28 -0.6 4,366,427 + Psyr_3680 0.34 +4.1 4,366,523 + Psyr_3680 0.42 -1.6 4,367,429 + Psyr_3681 0.20 +0.8 4,367,429 + Psyr_3681 0.20 -0.0 4,367,429 + Psyr_3681 0.20 -1.4 4,367,429 + Psyr_3681 0.20 -1.3 4,367,430 - Psyr_3681 0.20 -0.6 4,367,430 - Psyr_3681 0.20 -2.4 4,367,430 - Psyr_3681 0.20 +0.6 4,368,116 + +0.1 4,368,117 - +0.3 4,368,117 - +1.1
Or see this region's nucleotide sequence