Experiment: KB with Nonanoic 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2467 and Psyr_2468 overlap by 4 nucleotides Psyr_2468 and Psyr_2469 are separated by 14 nucleotides Psyr_2469 and Psyr_2470 are separated by 13 nucleotides
Psyr_2467: Psyr_2467 - 3-methylcrotonoyl-CoA carboxylase, alpha subunit, at 2,858,815 to 2,860,764
_2467
Psyr_2468: Psyr_2468 - Enoyl-CoA hydratase/isomerase, at 2,860,761 to 2,861,636
_2468
Psyr_2469: Psyr_2469 - Propionyl-CoA carboxylase, at 2,861,651 to 2,863,258
_2469
Psyr_2470: Psyr_2470 - isovaleryl-CoA dehydrogenase, at 2,863,272 to 2,864,435
_2470
Position (kb)
2861
2862
2863
2864 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2860.675 kb on - strand at 2861.140 kb on + strand, within Psyr_2468 at 2861.348 kb on - strand, within Psyr_2468 at 2861.392 kb on + strand, within Psyr_2468 at 2861.392 kb on + strand, within Psyr_2468 at 2861.392 kb on + strand, within Psyr_2468 at 2861.393 kb on - strand, within Psyr_2468 at 2861.393 kb on - strand, within Psyr_2468 at 2861.496 kb on + strand, within Psyr_2468 at 2861.496 kb on + strand, within Psyr_2468 at 2861.496 kb on + strand, within Psyr_2468 at 2861.610 kb on + strand at 2861.610 kb on + strand at 2861.610 kb on + strand at 2861.677 kb on + strand at 2861.677 kb on + strand at 2861.784 kb on + strand at 2861.784 kb on + strand at 2861.784 kb on + strand at 2861.806 kb on - strand at 2861.957 kb on + strand, within Psyr_2469 at 2861.957 kb on + strand, within Psyr_2469 at 2861.958 kb on - strand, within Psyr_2469 at 2861.973 kb on + strand, within Psyr_2469 at 2862.105 kb on + strand, within Psyr_2469 at 2862.105 kb on + strand, within Psyr_2469 at 2862.105 kb on + strand, within Psyr_2469 at 2862.106 kb on - strand, within Psyr_2469 at 2862.391 kb on - strand, within Psyr_2469 at 2862.837 kb on + strand, within Psyr_2469 at 2862.838 kb on - strand, within Psyr_2469 at 2863.016 kb on + strand, within Psyr_2469 at 2863.016 kb on + strand, within Psyr_2469 at 2863.016 kb on + strand, within Psyr_2469 at 2863.016 kb on + strand, within Psyr_2469 at 2863.016 kb on + strand, within Psyr_2469 at 2863.017 kb on - strand, within Psyr_2469 at 2863.017 kb on - strand, within Psyr_2469 at 2863.017 kb on - strand, within Psyr_2469 at 2863.017 kb on - strand, within Psyr_2469 at 2863.393 kb on + strand, within Psyr_2470 at 2863.393 kb on + strand, within Psyr_2470 at 2863.456 kb on + strand, within Psyr_2470 at 2863.555 kb on + strand, within Psyr_2470 at 2863.555 kb on + strand, within Psyr_2470 at 2863.555 kb on + strand, within Psyr_2470 at 2863.555 kb on + strand, within Psyr_2470 at 2863.556 kb on - strand, within Psyr_2470 at 2863.556 kb on - strand, within Psyr_2470 at 2863.556 kb on - strand, within Psyr_2470 at 2863.556 kb on - strand, within Psyr_2470 at 2863.556 kb on - strand, within Psyr_2470 at 2863.556 kb on - strand, within Psyr_2470 at 2863.556 kb on - strand, within Psyr_2470 at 2863.578 kb on + strand, within Psyr_2470 at 2863.578 kb on + strand, within Psyr_2470 at 2863.630 kb on + strand, within Psyr_2470 at 2863.631 kb on - strand, within Psyr_2470 at 2863.686 kb on + strand, within Psyr_2470 at 2863.915 kb on + strand, within Psyr_2470 at 2863.924 kb on + strand, within Psyr_2470 at 2863.924 kb on + strand, within Psyr_2470 at 2863.924 kb on + strand, within Psyr_2470 at 2863.924 kb on + strand, within Psyr_2470 at 2863.924 kb on + strand, within Psyr_2470 at 2863.924 kb on + strand, within Psyr_2470 at 2863.925 kb on - strand, within Psyr_2470 at 2863.925 kb on - strand, within Psyr_2470 at 2863.925 kb on - strand, within Psyr_2470 at 2863.925 kb on - strand, within Psyr_2470 at 2864.092 kb on + strand, within Psyr_2470
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nonanoic 10 mM remove 2,860,675 - -0.9 2,861,140 + Psyr_2468 0.43 +1.8 2,861,348 - Psyr_2468 0.67 +0.4 2,861,392 + Psyr_2468 0.72 +1.5 2,861,392 + Psyr_2468 0.72 +4.3 2,861,392 + Psyr_2468 0.72 +1.0 2,861,393 - Psyr_2468 0.72 +1.6 2,861,393 - Psyr_2468 0.72 -0.6 2,861,496 + Psyr_2468 0.84 -0.9 2,861,496 + Psyr_2468 0.84 +0.3 2,861,496 + Psyr_2468 0.84 +1.3 2,861,610 + +1.1 2,861,610 + +1.3 2,861,610 + +1.3 2,861,677 + +0.0 2,861,677 + -0.8 2,861,784 + +0.5 2,861,784 + -1.9 2,861,784 + +0.2 2,861,806 - -1.7 2,861,957 + Psyr_2469 0.19 +2.7 2,861,957 + Psyr_2469 0.19 +0.5 2,861,958 - Psyr_2469 0.19 +0.7 2,861,973 + Psyr_2469 0.20 -0.4 2,862,105 + Psyr_2469 0.28 +0.1 2,862,105 + Psyr_2469 0.28 -1.9 2,862,105 + Psyr_2469 0.28 -0.4 2,862,106 - Psyr_2469 0.28 +0.9 2,862,391 - Psyr_2469 0.46 -0.2 2,862,837 + Psyr_2469 0.74 +2.0 2,862,838 - Psyr_2469 0.74 -0.3 2,863,016 + Psyr_2469 0.85 +0.4 2,863,016 + Psyr_2469 0.85 +0.7 2,863,016 + Psyr_2469 0.85 +1.7 2,863,016 + Psyr_2469 0.85 -0.8 2,863,016 + Psyr_2469 0.85 +0.7 2,863,017 - Psyr_2469 0.85 -0.8 2,863,017 - Psyr_2469 0.85 +0.7 2,863,017 - Psyr_2469 0.85 -1.9 2,863,017 - Psyr_2469 0.85 -0.2 2,863,393 + Psyr_2470 0.10 +0.0 2,863,393 + Psyr_2470 0.10 -0.8 2,863,456 + Psyr_2470 0.16 -0.4 2,863,555 + Psyr_2470 0.24 -0.2 2,863,555 + Psyr_2470 0.24 +1.4 2,863,555 + Psyr_2470 0.24 -1.4 2,863,555 + Psyr_2470 0.24 -0.1 2,863,556 - Psyr_2470 0.24 -0.2 2,863,556 - Psyr_2470 0.24 +1.3 2,863,556 - Psyr_2470 0.24 +1.4 2,863,556 - Psyr_2470 0.24 +0.0 2,863,556 - Psyr_2470 0.24 +0.5 2,863,556 - Psyr_2470 0.24 +0.9 2,863,556 - Psyr_2470 0.24 +0.1 2,863,578 + Psyr_2470 0.26 +0.8 2,863,578 + Psyr_2470 0.26 +1.8 2,863,630 + Psyr_2470 0.31 -2.7 2,863,631 - Psyr_2470 0.31 -2.0 2,863,686 + Psyr_2470 0.36 +2.4 2,863,915 + Psyr_2470 0.55 +0.8 2,863,924 + Psyr_2470 0.56 +0.9 2,863,924 + Psyr_2470 0.56 +0.3 2,863,924 + Psyr_2470 0.56 +1.1 2,863,924 + Psyr_2470 0.56 -1.9 2,863,924 + Psyr_2470 0.56 -0.4 2,863,924 + Psyr_2470 0.56 -0.3 2,863,925 - Psyr_2470 0.56 -0.2 2,863,925 - Psyr_2470 0.56 -1.9 2,863,925 - Psyr_2470 0.56 +0.6 2,863,925 - Psyr_2470 0.56 +1.1 2,864,092 + Psyr_2470 0.70 +1.7
Or see this region's nucleotide sequence