Experiment: KB with Nonanoic 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1407 and Psyr_1408 are separated by 130 nucleotides Psyr_1408 and Psyr_1409 are separated by 85 nucleotides Psyr_1409 and Psyr_1410 are separated by 0 nucleotides
Psyr_1407: Psyr_1407 - Protein of unknown function DUF28, at 1,594,073 to 1,594,819
_1407
Psyr_1408: Psyr_1408 - Holliday junction endonuclease RuvC, at 1,594,950 to 1,595,474
_1408
Psyr_1409: Psyr_1409 - Holliday junction DNA helicase subunit RuvA, at 1,595,560 to 1,596,168
_1409
Psyr_1410: Psyr_1410 - Holliday junction DNA helicase RuvB, at 1,596,169 to 1,597,230
_1410
Position (kb)
1595
1596
1597 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1594.613 kb on + strand, within Psyr_1407 at 1594.614 kb on - strand, within Psyr_1407 at 1594.738 kb on + strand, within Psyr_1407 at 1594.739 kb on - strand, within Psyr_1407 at 1594.739 kb on - strand, within Psyr_1407 at 1594.772 kb on + strand at 1594.772 kb on + strand at 1594.832 kb on - strand at 1594.903 kb on + strand at 1594.903 kb on + strand at 1594.904 kb on - strand at 1594.914 kb on + strand at 1594.914 kb on + strand at 1594.914 kb on + strand at 1594.914 kb on + strand at 1594.914 kb on + strand at 1594.914 kb on + strand at 1594.958 kb on + strand at 1594.958 kb on + strand at 1594.958 kb on + strand at 1594.959 kb on - strand at 1594.959 kb on - strand at 1594.959 kb on - strand at 1595.032 kb on - strand, within Psyr_1408 at 1595.032 kb on - strand, within Psyr_1408 at 1595.032 kb on - strand, within Psyr_1408 at 1595.125 kb on - strand, within Psyr_1408 at 1595.125 kb on - strand, within Psyr_1408 at 1595.254 kb on - strand, within Psyr_1408 at 1595.405 kb on + strand, within Psyr_1408 at 1595.481 kb on + strand at 1595.481 kb on + strand at 1595.481 kb on + strand at 1595.481 kb on + strand at 1595.481 kb on + strand at 1595.481 kb on + strand at 1595.481 kb on + strand at 1595.482 kb on - strand at 1595.482 kb on - strand at 1595.482 kb on - strand at 1595.482 kb on - strand at 1595.482 kb on - strand at 1595.482 kb on - strand at 1595.482 kb on - strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.494 kb on - strand at 1595.500 kb on + strand at 1595.500 kb on + strand at 1595.501 kb on - strand at 1595.573 kb on + strand at 1595.855 kb on + strand, within Psyr_1409 at 1595.855 kb on + strand, within Psyr_1409 at 1595.864 kb on + strand, within Psyr_1409 at 1595.865 kb on - strand, within Psyr_1409 at 1596.167 kb on - strand at 1596.192 kb on - strand at 1596.426 kb on + strand, within Psyr_1410 at 1596.427 kb on - strand, within Psyr_1410 at 1596.427 kb on - strand, within Psyr_1410 at 1596.427 kb on - strand, within Psyr_1410 at 1596.427 kb on - strand, within Psyr_1410 at 1596.427 kb on - strand, within Psyr_1410 at 1596.551 kb on - strand, within Psyr_1410 at 1596.721 kb on + strand, within Psyr_1410 at 1597.082 kb on - strand, within Psyr_1410 at 1597.082 kb on - strand, within Psyr_1410 at 1597.099 kb on + strand, within Psyr_1410 at 1597.099 kb on + strand, within Psyr_1410 at 1597.100 kb on - strand, within Psyr_1410 at 1597.100 kb on - strand, within Psyr_1410 at 1597.101 kb on + strand, within Psyr_1410 at 1597.102 kb on - strand, within Psyr_1410 at 1597.165 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nonanoic 10 mM remove 1,594,613 + Psyr_1407 0.72 +0.9 1,594,614 - Psyr_1407 0.72 +0.5 1,594,738 + Psyr_1407 0.89 -0.4 1,594,739 - Psyr_1407 0.89 -1.5 1,594,739 - Psyr_1407 0.89 -1.0 1,594,772 + +2.2 1,594,772 + +1.9 1,594,832 - +1.2 1,594,903 + +0.1 1,594,903 + +1.8 1,594,904 - -1.7 1,594,914 + -3.4 1,594,914 + +1.6 1,594,914 + +1.2 1,594,914 + -0.6 1,594,914 + -0.1 1,594,914 + +1.2 1,594,958 + -0.1 1,594,958 + +2.4 1,594,958 + -1.5 1,594,959 - -1.2 1,594,959 - +0.3 1,594,959 - -0.8 1,595,032 - Psyr_1408 0.16 -1.1 1,595,032 - Psyr_1408 0.16 -0.0 1,595,032 - Psyr_1408 0.16 +0.0 1,595,125 - Psyr_1408 0.33 -0.8 1,595,125 - Psyr_1408 0.33 -2.1 1,595,254 - Psyr_1408 0.58 -2.1 1,595,405 + Psyr_1408 0.87 -0.8 1,595,481 + -0.8 1,595,481 + +2.2 1,595,481 + +1.8 1,595,481 + +0.0 1,595,481 + +1.1 1,595,481 + -0.3 1,595,481 + -0.1 1,595,482 - +0.2 1,595,482 - -3.6 1,595,482 - -1.8 1,595,482 - +1.2 1,595,482 - +0.3 1,595,482 - -1.8 1,595,482 - +1.8 1,595,483 + +0.6 1,595,483 + +1.2 1,595,483 + +0.6 1,595,483 + -0.0 1,595,483 + -2.1 1,595,483 + +0.8 1,595,483 + -1.0 1,595,483 + +0.5 1,595,483 + -0.1 1,595,483 + -0.3 1,595,483 + +0.1 1,595,483 + +0.4 1,595,483 + -2.0 1,595,483 + -0.4 1,595,483 + -0.2 1,595,484 - +1.4 1,595,484 - -1.0 1,595,484 - -0.1 1,595,484 - -1.8 1,595,484 - -1.6 1,595,484 - +0.4 1,595,484 - -0.4 1,595,484 - +0.6 1,595,494 - +0.6 1,595,500 + +0.1 1,595,500 + +3.8 1,595,501 - -1.1 1,595,573 + -2.0 1,595,855 + Psyr_1409 0.48 -1.6 1,595,855 + Psyr_1409 0.48 -1.0 1,595,864 + Psyr_1409 0.50 -2.4 1,595,865 - Psyr_1409 0.50 -2.5 1,596,167 - +0.5 1,596,192 - -1.8 1,596,426 + Psyr_1410 0.24 -2.6 1,596,427 - Psyr_1410 0.24 -1.7 1,596,427 - Psyr_1410 0.24 -0.8 1,596,427 - Psyr_1410 0.24 +0.2 1,596,427 - Psyr_1410 0.24 -1.7 1,596,427 - Psyr_1410 0.24 -0.0 1,596,551 - Psyr_1410 0.36 -1.0 1,596,721 + Psyr_1410 0.52 +3.8 1,597,082 - Psyr_1410 0.86 -3.1 1,597,082 - Psyr_1410 0.86 -2.0 1,597,099 + Psyr_1410 0.88 +2.2 1,597,099 + Psyr_1410 0.88 -0.4 1,597,100 - Psyr_1410 0.88 -2.3 1,597,100 - Psyr_1410 0.88 +1.2 1,597,101 + Psyr_1410 0.88 +0.7 1,597,102 - Psyr_1410 0.88 -0.4 1,597,165 - +0.3
Or see this region's nucleotide sequence