Experiment: KB with Nonanoic 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0032 and Psyr_0033 are separated by 125 nucleotides Psyr_0033 and Psyr_0034 overlap by 4 nucleotides Psyr_0034 and Psyr_0035 are separated by 79 nucleotides
Psyr_0032: Psyr_0032 - conserved hypothetical protein, at 36,536 to 37,066
_0032
Psyr_0033: Psyr_0033 - tryptophan synthase, alpha chain, at 37,192 to 38,001
_0033
Psyr_0034: Psyr_0034 - tryptophan synthase, beta chain, at 37,998 to 39,227
_0034
Psyr_0035: Psyr_0035 - regulatory protein, LysR:LysR, substrate-binding protein, at 39,307 to 40,263
_0035
Position (kb) 37
38
39
40 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 37.030 kb on + strand at 37.030 kb on + strand at 37.045 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.144 kb on + strand at 37.144 kb on + strand at 37.144 kb on + strand at 37.144 kb on + strand at 37.144 kb on + strand at 37.144 kb on + strand at 37.144 kb on + strand at 37.144 kb on + strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.175 kb on + strand at 37.210 kb on + strand at 37.210 kb on + strand at 37.210 kb on + strand at 37.210 kb on + strand at 37.210 kb on + strand at 37.211 kb on - strand at 37.211 kb on - strand at 37.211 kb on - strand at 37.475 kb on + strand, within Psyr_0033 at 37.475 kb on + strand, within Psyr_0033 at 37.475 kb on + strand, within Psyr_0033 at 37.475 kb on + strand, within Psyr_0033 at 37.476 kb on - strand, within Psyr_0033 at 37.476 kb on - strand, within Psyr_0033 at 37.476 kb on - strand, within Psyr_0033 at 37.479 kb on - strand, within Psyr_0033 at 37.479 kb on - strand, within Psyr_0033 at 37.694 kb on + strand, within Psyr_0033 at 37.694 kb on + strand, within Psyr_0033 at 37.694 kb on + strand, within Psyr_0033 at 37.694 kb on + strand, within Psyr_0033 at 37.695 kb on - strand, within Psyr_0033 at 37.695 kb on - strand, within Psyr_0033 at 37.695 kb on - strand, within Psyr_0033 at 37.695 kb on - strand, within Psyr_0033 at 37.695 kb on - strand, within Psyr_0033 at 37.695 kb on - strand, within Psyr_0033 at 37.695 kb on - strand, within Psyr_0033 at 37.697 kb on + strand, within Psyr_0033 at 37.969 kb on - strand at 37.969 kb on - strand at 37.969 kb on - strand at 37.991 kb on - strand at 38.350 kb on + strand, within Psyr_0034 at 38.351 kb on - strand, within Psyr_0034 at 38.351 kb on - strand, within Psyr_0034 at 38.647 kb on + strand, within Psyr_0034 at 38.648 kb on - strand, within Psyr_0034 at 38.648 kb on - strand, within Psyr_0034 at 38.806 kb on + strand, within Psyr_0034 at 38.806 kb on + strand, within Psyr_0034 at 38.807 kb on - strand, within Psyr_0034 at 38.807 kb on - strand, within Psyr_0034 at 38.807 kb on - strand, within Psyr_0034 at 38.807 kb on - strand, within Psyr_0034 at 38.807 kb on - strand, within Psyr_0034 at 38.807 kb on - strand, within Psyr_0034 at 38.807 kb on - strand, within Psyr_0034 at 38.919 kb on + strand, within Psyr_0034 at 38.919 kb on + strand, within Psyr_0034 at 38.983 kb on + strand, within Psyr_0034 at 38.984 kb on - strand, within Psyr_0034 at 38.984 kb on - strand, within Psyr_0034 at 39.028 kb on + strand, within Psyr_0034 at 39.068 kb on - strand, within Psyr_0034 at 39.068 kb on - strand, within Psyr_0034 at 39.160 kb on + strand at 39.160 kb on + strand at 39.257 kb on + strand at 39.258 kb on - strand at 39.270 kb on - strand at 39.502 kb on - strand, within Psyr_0035 at 39.602 kb on + strand, within Psyr_0035 at 39.602 kb on + strand, within Psyr_0035 at 39.602 kb on + strand, within Psyr_0035 at 39.602 kb on + strand, within Psyr_0035 at 39.602 kb on + strand, within Psyr_0035 at 39.602 kb on + strand, within Psyr_0035 at 39.602 kb on + strand, within Psyr_0035 at 39.602 kb on + strand, within Psyr_0035 at 39.602 kb on + strand, within Psyr_0035 at 39.602 kb on + strand, within Psyr_0035 at 39.602 kb on + strand, within Psyr_0035 at 39.602 kb on + strand, within Psyr_0035 at 39.603 kb on - strand, within Psyr_0035 at 39.603 kb on - strand, within Psyr_0035 at 39.603 kb on - strand, within Psyr_0035 at 39.603 kb on - strand, within Psyr_0035 at 39.603 kb on - strand, within Psyr_0035 at 39.603 kb on - strand, within Psyr_0035 at 39.603 kb on - strand, within Psyr_0035 at 39.792 kb on - strand, within Psyr_0035 at 40.033 kb on + strand, within Psyr_0035 at 40.033 kb on + strand, within Psyr_0035 at 40.033 kb on + strand, within Psyr_0035 at 40.034 kb on - strand, within Psyr_0035 at 40.034 kb on - strand, within Psyr_0035 at 40.034 kb on - strand, within Psyr_0035 at 40.036 kb on + strand, within Psyr_0035 at 40.036 kb on + strand, within Psyr_0035 at 40.036 kb on + strand, within Psyr_0035 at 40.037 kb on - strand, within Psyr_0035 at 40.037 kb on - strand, within Psyr_0035 at 40.037 kb on - strand, within Psyr_0035 at 40.037 kb on - strand, within Psyr_0035 at 40.037 kb on - strand, within Psyr_0035 at 40.037 kb on - strand, within Psyr_0035
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nonanoic 10 mM remove 37,030 + +0.2 37,030 + +0.1 37,045 + -1.0 37,140 + -0.8 37,140 + +0.7 37,140 + +0.0 37,140 + -1.2 37,141 - -0.8 37,141 - +0.3 37,141 - -0.4 37,141 - -1.4 37,141 - -0.6 37,141 - +0.0 37,141 - -0.5 37,141 - -1.3 37,141 - -0.9 37,144 + +3.0 37,144 + +0.0 37,144 + +0.5 37,144 + +0.7 37,144 + +0.4 37,144 + -0.0 37,144 + +0.5 37,144 + +0.5 37,145 - -1.5 37,145 - -0.9 37,145 - -1.3 37,145 - -1.9 37,175 + -0.9 37,210 + -0.6 37,210 + -1.6 37,210 + -0.8 37,210 + +0.1 37,210 + +3.7 37,211 - +1.3 37,211 - +0.4 37,211 - -1.2 37,475 + Psyr_0033 0.35 -3.0 37,475 + Psyr_0033 0.35 +1.4 37,475 + Psyr_0033 0.35 -0.9 37,475 + Psyr_0033 0.35 -2.8 37,476 - Psyr_0033 0.35 -1.6 37,476 - Psyr_0033 0.35 -3.4 37,476 - Psyr_0033 0.35 -3.2 37,479 - Psyr_0033 0.35 -0.9 37,479 - Psyr_0033 0.35 -2.5 37,694 + Psyr_0033 0.62 -3.1 37,694 + Psyr_0033 0.62 -2.5 37,694 + Psyr_0033 0.62 -4.2 37,694 + Psyr_0033 0.62 -2.9 37,695 - Psyr_0033 0.62 -3.0 37,695 - Psyr_0033 0.62 -3.2 37,695 - Psyr_0033 0.62 -1.7 37,695 - Psyr_0033 0.62 -3.3 37,695 - Psyr_0033 0.62 +3.4 37,695 - Psyr_0033 0.62 -1.4 37,695 - Psyr_0033 0.62 +0.1 37,697 + Psyr_0033 0.62 -2.3 37,969 - -1.7 37,969 - -3.6 37,969 - -1.7 37,991 - -1.0 38,350 + Psyr_0034 0.29 -3.4 38,351 - Psyr_0034 0.29 -1.3 38,351 - Psyr_0034 0.29 -3.2 38,647 + Psyr_0034 0.53 -1.9 38,648 - Psyr_0034 0.53 -0.6 38,648 - Psyr_0034 0.53 +1.4 38,806 + Psyr_0034 0.66 -2.6 38,806 + Psyr_0034 0.66 -2.2 38,807 - Psyr_0034 0.66 -1.0 38,807 - Psyr_0034 0.66 -1.4 38,807 - Psyr_0034 0.66 -2.8 38,807 - Psyr_0034 0.66 -0.6 38,807 - Psyr_0034 0.66 -2.3 38,807 - Psyr_0034 0.66 -2.0 38,807 - Psyr_0034 0.66 -1.7 38,919 + Psyr_0034 0.75 -1.4 38,919 + Psyr_0034 0.75 -1.6 38,983 + Psyr_0034 0.80 -2.9 38,984 - Psyr_0034 0.80 -2.6 38,984 - Psyr_0034 0.80 +1.4 39,028 + Psyr_0034 0.84 -2.7 39,068 - Psyr_0034 0.87 -2.2 39,068 - Psyr_0034 0.87 +0.4 39,160 + -1.1 39,160 + -2.6 39,257 + +0.1 39,258 - -0.7 39,270 - -1.4 39,502 - Psyr_0035 0.20 +0.6 39,602 + Psyr_0035 0.31 +2.4 39,602 + Psyr_0035 0.31 -0.2 39,602 + Psyr_0035 0.31 -0.5 39,602 + Psyr_0035 0.31 -0.5 39,602 + Psyr_0035 0.31 -2.7 39,602 + Psyr_0035 0.31 -1.6 39,602 + Psyr_0035 0.31 +0.2 39,602 + Psyr_0035 0.31 +3.7 39,602 + Psyr_0035 0.31 +0.3 39,602 + Psyr_0035 0.31 +0.4 39,602 + Psyr_0035 0.31 -0.8 39,602 + Psyr_0035 0.31 -0.3 39,603 - Psyr_0035 0.31 -0.7 39,603 - Psyr_0035 0.31 +0.5 39,603 - Psyr_0035 0.31 -0.3 39,603 - Psyr_0035 0.31 -0.6 39,603 - Psyr_0035 0.31 +1.6 39,603 - Psyr_0035 0.31 -0.8 39,603 - Psyr_0035 0.31 -0.9 39,792 - Psyr_0035 0.51 +0.7 40,033 + Psyr_0035 0.76 -1.2 40,033 + Psyr_0035 0.76 +0.7 40,033 + Psyr_0035 0.76 -1.2 40,034 - Psyr_0035 0.76 -0.2 40,034 - Psyr_0035 0.76 +0.5 40,034 - Psyr_0035 0.76 +0.3 40,036 + Psyr_0035 0.76 +0.1 40,036 + Psyr_0035 0.76 -0.2 40,036 + Psyr_0035 0.76 +1.3 40,037 - Psyr_0035 0.76 -1.4 40,037 - Psyr_0035 0.76 +0.8 40,037 - Psyr_0035 0.76 +0.5 40,037 - Psyr_0035 0.76 +0.2 40,037 - Psyr_0035 0.76 +0.1 40,037 - Psyr_0035 0.76 -1.0
Or see this region's nucleotide sequence