Experiment: RCH2_defined_glucose with MS_media 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_25270 and PS417_25275 are separated by 140 nucleotides PS417_25275 and PS417_25280 overlap by 4 nucleotides PS417_25280 and PS417_25285 are separated by 151 nucleotides
PS417_25270: PS417_25270 - NrdR family transcriptional regulator, at 5,474,400 to 5,474,864
_25270
PS417_25275: PS417_25275 - hypothetical protein, at 5,475,005 to 5,475,460
_25275
PS417_25280: PS417_25280 - methyltransferase, at 5,475,457 to 5,476,113
_25280
PS417_25285: PS417_25285 - thioredoxin, at 5,476,265 to 5,477,137
_25285
Position (kb) 5475
5476
5477 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5474.495 kb on - strand, within PS417_25270 at 5474.495 kb on - strand, within PS417_25270 at 5474.495 kb on - strand, within PS417_25270 at 5474.495 kb on - strand, within PS417_25270 at 5474.495 kb on - strand, within PS417_25270 at 5474.725 kb on - strand, within PS417_25270 at 5474.883 kb on - strand at 5474.883 kb on - strand at 5474.883 kb on - strand at 5474.935 kb on - strand at 5474.939 kb on + strand at 5474.939 kb on + strand at 5474.940 kb on - strand at 5474.940 kb on - strand at 5474.941 kb on + strand at 5474.942 kb on - strand at 5474.942 kb on - strand at 5474.943 kb on + strand at 5474.943 kb on + strand at 5474.943 kb on + strand at 5474.943 kb on + strand at 5474.944 kb on - strand at 5474.944 kb on - strand at 5474.944 kb on - strand at 5474.944 kb on - strand at 5474.953 kb on - strand at 5474.955 kb on + strand at 5474.956 kb on - strand at 5474.956 kb on - strand at 5475.002 kb on + strand at 5475.002 kb on + strand at 5475.002 kb on + strand at 5475.003 kb on - strand at 5475.003 kb on - strand at 5475.003 kb on - strand at 5475.267 kb on + strand, within PS417_25275 at 5475.267 kb on + strand, within PS417_25275 at 5475.268 kb on - strand, within PS417_25275 at 5475.552 kb on - strand, within PS417_25280 at 5475.671 kb on - strand, within PS417_25280 at 5475.853 kb on + strand, within PS417_25280 at 5475.916 kb on + strand, within PS417_25280 at 5475.916 kb on + strand, within PS417_25280 at 5476.085 kb on + strand at 5476.086 kb on - strand at 5476.093 kb on + strand at 5476.228 kb on + strand at 5476.230 kb on + strand at 5476.230 kb on + strand at 5476.230 kb on + strand at 5476.231 kb on - strand at 5476.231 kb on - strand at 5476.231 kb on - strand at 5476.231 kb on - strand at 5476.250 kb on + strand at 5476.250 kb on + strand at 5476.283 kb on + strand at 5476.283 kb on + strand at 5476.427 kb on + strand, within PS417_25285 at 5476.427 kb on + strand, within PS417_25285 at 5476.428 kb on - strand, within PS417_25285 at 5476.602 kb on + strand, within PS417_25285 at 5476.602 kb on + strand, within PS417_25285 at 5476.602 kb on + strand, within PS417_25285 at 5476.602 kb on + strand, within PS417_25285 at 5476.603 kb on - strand, within PS417_25285 at 5476.603 kb on - strand, within PS417_25285 at 5476.603 kb on - strand, within PS417_25285 at 5476.603 kb on - strand, within PS417_25285 at 5476.603 kb on - strand, within PS417_25285 at 5476.603 kb on - strand, within PS417_25285 at 5476.603 kb on - strand, within PS417_25285 at 5476.603 kb on - strand, within PS417_25285 at 5476.718 kb on - strand, within PS417_25285 at 5476.941 kb on - strand, within PS417_25285 at 5477.027 kb on + strand, within PS417_25285
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 1x remove 5,474,495 - PS417_25270 0.20 +0.0 5,474,495 - PS417_25270 0.20 +0.6 5,474,495 - PS417_25270 0.20 +1.0 5,474,495 - PS417_25270 0.20 +0.2 5,474,495 - PS417_25270 0.20 -0.5 5,474,725 - PS417_25270 0.70 -1.2 5,474,883 - +0.4 5,474,883 - +1.0 5,474,883 - -0.2 5,474,935 - -0.9 5,474,939 + -0.2 5,474,939 + +2.7 5,474,940 - -1.2 5,474,940 - +0.3 5,474,941 + +0.1 5,474,942 - +0.5 5,474,942 - -0.0 5,474,943 + -0.2 5,474,943 + -1.0 5,474,943 + -0.0 5,474,943 + -0.9 5,474,944 - -2.0 5,474,944 - -0.5 5,474,944 - -0.6 5,474,944 - -0.1 5,474,953 - +0.1 5,474,955 + -0.6 5,474,956 - +2.0 5,474,956 - +0.3 5,475,002 + -0.3 5,475,002 + +1.2 5,475,002 + +0.6 5,475,003 - +0.6 5,475,003 - +2.0 5,475,003 - +0.2 5,475,267 + PS417_25275 0.57 -0.3 5,475,267 + PS417_25275 0.57 -0.3 5,475,268 - PS417_25275 0.58 +0.2 5,475,552 - PS417_25280 0.14 +0.9 5,475,671 - PS417_25280 0.33 -0.3 5,475,853 + PS417_25280 0.60 +0.4 5,475,916 + PS417_25280 0.70 +0.3 5,475,916 + PS417_25280 0.70 +0.7 5,476,085 + +0.1 5,476,086 - +1.5 5,476,093 + -0.0 5,476,228 + +2.0 5,476,230 + -1.9 5,476,230 + -0.5 5,476,230 + -3.0 5,476,231 - +0.2 5,476,231 - -0.3 5,476,231 - -1.6 5,476,231 - +0.7 5,476,250 + +1.1 5,476,250 + +0.7 5,476,283 + -2.6 5,476,283 + +0.3 5,476,427 + PS417_25285 0.19 +0.2 5,476,427 + PS417_25285 0.19 +0.1 5,476,428 - PS417_25285 0.19 -0.7 5,476,602 + PS417_25285 0.39 +0.2 5,476,602 + PS417_25285 0.39 -1.1 5,476,602 + PS417_25285 0.39 +0.1 5,476,602 + PS417_25285 0.39 +0.1 5,476,603 - PS417_25285 0.39 -0.7 5,476,603 - PS417_25285 0.39 +0.0 5,476,603 - PS417_25285 0.39 -0.3 5,476,603 - PS417_25285 0.39 -0.4 5,476,603 - PS417_25285 0.39 -0.5 5,476,603 - PS417_25285 0.39 -2.5 5,476,603 - PS417_25285 0.39 -0.1 5,476,603 - PS417_25285 0.39 -0.0 5,476,718 - PS417_25285 0.52 -0.6 5,476,941 - PS417_25285 0.77 +0.2 5,477,027 + PS417_25285 0.87 +0.1
Or see this region's nucleotide sequence