Experiment: RCH2_defined_glucose with MS_media 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_16215 and PS417_16220 are separated by 32 nucleotides PS417_16220 and PS417_16225 are separated by 10 nucleotides PS417_16225 and PS417_16230 are separated by 10 nucleotides PS417_16230 and PS417_16235 overlap by 4 nucleotides
PS417_16215: PS417_16215 - ABC transporter substrate-binding protein, at 3,553,549 to 3,555,291
_16215
PS417_16220: PS417_16220 - membrane protein, at 3,555,324 to 3,555,596
_16220
PS417_16225: PS417_16225 - sugar ABC transporter permease, at 3,555,607 to 3,556,407
_16225
PS417_16230: PS417_16230 - ABC transporter permease, at 3,556,418 to 3,557,284
_16230
PS417_16235: PS417_16235 - ABC transporter ATP-binding protein, at 3,557,281 to 3,558,378
_16235
Position (kb)
3555
3556
3557 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3554.609 kb on + strand, within PS417_16215 at 3554.741 kb on + strand at 3554.742 kb on - strand, within PS417_16215 at 3554.742 kb on - strand, within PS417_16215 at 3554.912 kb on + strand, within PS417_16215 at 3554.913 kb on - strand, within PS417_16215 at 3554.913 kb on - strand, within PS417_16215 at 3554.913 kb on - strand, within PS417_16215 at 3554.913 kb on - strand, within PS417_16215 at 3554.913 kb on - strand, within PS417_16215 at 3555.109 kb on - strand, within PS417_16215 at 3555.262 kb on - strand at 3555.273 kb on + strand at 3555.282 kb on + strand at 3555.283 kb on - strand at 3555.291 kb on + strand at 3555.292 kb on - strand at 3555.310 kb on + strand at 3555.422 kb on - strand, within PS417_16220 at 3555.681 kb on + strand at 3555.681 kb on + strand at 3556.043 kb on + strand, within PS417_16225 at 3556.043 kb on + strand, within PS417_16225 at 3556.044 kb on - strand, within PS417_16225 at 3556.198 kb on + strand, within PS417_16225 at 3556.198 kb on + strand, within PS417_16225 at 3556.198 kb on + strand, within PS417_16225 at 3556.199 kb on - strand, within PS417_16225 at 3556.199 kb on - strand, within PS417_16225 at 3556.202 kb on + strand, within PS417_16225 at 3556.202 kb on + strand, within PS417_16225 at 3556.203 kb on - strand, within PS417_16225 at 3556.205 kb on + strand, within PS417_16225 at 3556.230 kb on - strand at 3556.259 kb on + strand, within PS417_16225 at 3556.373 kb on + strand at 3556.373 kb on + strand at 3556.373 kb on + strand at 3556.374 kb on - strand at 3556.374 kb on - strand at 3556.374 kb on - strand at 3556.402 kb on - strand at 3556.488 kb on + strand at 3556.489 kb on - strand at 3556.532 kb on + strand at 3556.567 kb on + strand, within PS417_16230 at 3556.567 kb on + strand, within PS417_16230 at 3556.568 kb on - strand, within PS417_16230 at 3556.571 kb on - strand, within PS417_16230 at 3556.614 kb on + strand, within PS417_16230 at 3556.634 kb on - strand, within PS417_16230 at 3556.688 kb on - strand, within PS417_16230 at 3556.688 kb on - strand, within PS417_16230 at 3556.688 kb on - strand, within PS417_16230 at 3556.740 kb on + strand, within PS417_16230 at 3556.741 kb on - strand, within PS417_16230 at 3556.805 kb on + strand, within PS417_16230 at 3556.858 kb on + strand, within PS417_16230 at 3556.861 kb on - strand, within PS417_16230 at 3556.887 kb on + strand, within PS417_16230 at 3557.125 kb on + strand, within PS417_16230 at 3557.126 kb on - strand, within PS417_16230 at 3557.127 kb on + strand, within PS417_16230 at 3557.127 kb on + strand, within PS417_16230 at 3557.128 kb on - strand, within PS417_16230 at 3557.148 kb on + strand, within PS417_16230 at 3557.184 kb on + strand, within PS417_16230 at 3557.185 kb on - strand, within PS417_16230 at 3557.303 kb on + strand at 3557.316 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 1x remove 3,554,609 + PS417_16215 0.61 -0.3 3,554,741 + +0.2 3,554,742 - PS417_16215 0.68 -1.6 3,554,742 - PS417_16215 0.68 +0.4 3,554,912 + PS417_16215 0.78 +0.0 3,554,913 - PS417_16215 0.78 -0.5 3,554,913 - PS417_16215 0.78 +0.7 3,554,913 - PS417_16215 0.78 -1.1 3,554,913 - PS417_16215 0.78 -3.1 3,554,913 - PS417_16215 0.78 -0.0 3,555,109 - PS417_16215 0.90 -0.7 3,555,262 - -1.0 3,555,273 + -0.5 3,555,282 + -0.7 3,555,283 - +0.1 3,555,291 + -0.7 3,555,292 - -0.4 3,555,310 + +0.4 3,555,422 - PS417_16220 0.36 -0.5 3,555,681 + +0.2 3,555,681 + -0.7 3,556,043 + PS417_16225 0.54 -0.0 3,556,043 + PS417_16225 0.54 +0.4 3,556,044 - PS417_16225 0.55 +0.5 3,556,198 + PS417_16225 0.74 +0.1 3,556,198 + PS417_16225 0.74 +2.4 3,556,198 + PS417_16225 0.74 -0.3 3,556,199 - PS417_16225 0.74 -0.6 3,556,199 - PS417_16225 0.74 +0.9 3,556,202 + PS417_16225 0.74 +1.9 3,556,202 + PS417_16225 0.74 +1.0 3,556,203 - PS417_16225 0.74 +0.6 3,556,205 + PS417_16225 0.75 +0.3 3,556,230 - +0.9 3,556,259 + PS417_16225 0.81 +0.2 3,556,373 + -1.5 3,556,373 + -1.6 3,556,373 + -0.9 3,556,374 - -0.4 3,556,374 - -1.5 3,556,374 - -0.1 3,556,402 - -0.1 3,556,488 + -0.3 3,556,489 - +1.3 3,556,532 + -0.9 3,556,567 + PS417_16230 0.17 +0.0 3,556,567 + PS417_16230 0.17 +1.3 3,556,568 - PS417_16230 0.17 -0.6 3,556,571 - PS417_16230 0.18 +1.1 3,556,614 + PS417_16230 0.23 -0.1 3,556,634 - PS417_16230 0.25 +0.9 3,556,688 - PS417_16230 0.31 -0.1 3,556,688 - PS417_16230 0.31 +0.4 3,556,688 - PS417_16230 0.31 -0.8 3,556,740 + PS417_16230 0.37 +1.1 3,556,741 - PS417_16230 0.37 -0.9 3,556,805 + PS417_16230 0.45 +2.5 3,556,858 + PS417_16230 0.51 +0.2 3,556,861 - PS417_16230 0.51 +0.2 3,556,887 + PS417_16230 0.54 +0.7 3,557,125 + PS417_16230 0.82 -1.7 3,557,126 - PS417_16230 0.82 +0.7 3,557,127 + PS417_16230 0.82 +2.9 3,557,127 + PS417_16230 0.82 +2.4 3,557,128 - PS417_16230 0.82 -0.3 3,557,148 + PS417_16230 0.84 -1.7 3,557,184 + PS417_16230 0.88 +0.4 3,557,185 - PS417_16230 0.88 +0.4 3,557,303 + +0.0 3,557,316 - +0.5
Or see this region's nucleotide sequence