Experiment: RCH2_defined_glucose with MS_media 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12905 and PS417_12910 are separated by 286 nucleotides PS417_12910 and PS417_12915 are separated by 70 nucleotides PS417_12915 and PS417_12920 overlap by 13 nucleotides
PS417_12905: PS417_12905 - ribonucleotide-diphosphate reductase subunit beta, at 2,823,069 to 2,824,319
_12905
PS417_12910: PS417_12910 - alanine racemase, at 2,824,606 to 2,825,703
_12910
PS417_12915: PS417_12915 - DNA-binding protein, at 2,825,774 to 2,826,400
_12915
PS417_12920: PS417_12920 - endoribonuclease, at 2,826,388 to 2,826,768
_12920
Position (kb)
2824
2825
2826 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2824.325 kb on + strand at 2824.388 kb on - strand at 2824.388 kb on - strand at 2824.390 kb on + strand at 2824.390 kb on + strand at 2824.391 kb on - strand at 2824.403 kb on - strand at 2824.423 kb on + strand at 2824.434 kb on - strand at 2824.443 kb on + strand at 2824.444 kb on - strand at 2824.444 kb on - strand at 2824.448 kb on - strand at 2824.448 kb on - strand at 2824.475 kb on + strand at 2824.475 kb on + strand at 2824.475 kb on + strand at 2824.476 kb on - strand at 2824.476 kb on - strand at 2824.476 kb on - strand at 2824.476 kb on - strand at 2824.476 kb on - strand at 2824.477 kb on + strand at 2824.477 kb on + strand at 2824.477 kb on + strand at 2824.477 kb on + strand at 2824.478 kb on - strand at 2824.478 kb on - strand at 2824.604 kb on + strand at 2824.605 kb on - strand at 2824.630 kb on + strand at 2824.631 kb on - strand at 2824.636 kb on + strand at 2824.636 kb on + strand at 2824.636 kb on + strand at 2824.664 kb on + strand at 2824.987 kb on + strand, within PS417_12910 at 2824.988 kb on - strand, within PS417_12910 at 2825.009 kb on - strand, within PS417_12910 at 2825.009 kb on - strand, within PS417_12910 at 2825.009 kb on - strand, within PS417_12910 at 2825.009 kb on - strand at 2825.762 kb on - strand at 2825.783 kb on - strand at 2825.783 kb on - strand at 2826.083 kb on + strand, within PS417_12915 at 2826.084 kb on - strand, within PS417_12915 at 2826.289 kb on - strand, within PS417_12915 at 2826.289 kb on - strand, within PS417_12915 at 2826.384 kb on - strand at 2826.389 kb on + strand at 2826.389 kb on + strand at 2826.398 kb on + strand at 2826.539 kb on + strand, within PS417_12920 at 2826.539 kb on + strand, within PS417_12920 at 2826.539 kb on + strand, within PS417_12920 at 2826.540 kb on - strand, within PS417_12920 at 2826.540 kb on - strand, within PS417_12920 at 2826.540 kb on - strand, within PS417_12920 at 2826.598 kb on + strand, within PS417_12920 at 2826.689 kb on + strand, within PS417_12920 at 2826.690 kb on - strand, within PS417_12920 at 2826.690 kb on - strand, within PS417_12920 at 2826.690 kb on - strand, within PS417_12920 at 2826.690 kb on - strand, within PS417_12920
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 1x remove 2,824,325 + -0.1 2,824,388 - +1.7 2,824,388 - +0.6 2,824,390 + -0.0 2,824,390 + +0.4 2,824,391 - -0.8 2,824,403 - -0.2 2,824,423 + -0.1 2,824,434 - +0.2 2,824,443 + -0.1 2,824,444 - +0.9 2,824,444 - +0.8 2,824,448 - +0.4 2,824,448 - +0.2 2,824,475 + +0.6 2,824,475 + +0.9 2,824,475 + -0.3 2,824,476 - -1.0 2,824,476 - -0.7 2,824,476 - -0.4 2,824,476 - -0.2 2,824,476 - +1.5 2,824,477 + +0.2 2,824,477 + -1.2 2,824,477 + -0.1 2,824,477 + -1.1 2,824,478 - -1.1 2,824,478 - +0.2 2,824,604 + +0.9 2,824,605 - +0.1 2,824,630 + +0.9 2,824,631 - +0.0 2,824,636 + -1.1 2,824,636 + +0.8 2,824,636 + -0.2 2,824,664 + +0.9 2,824,987 + PS417_12910 0.35 +1.9 2,824,988 - PS417_12910 0.35 -0.7 2,825,009 - PS417_12910 0.37 -2.5 2,825,009 - PS417_12910 0.37 +0.5 2,825,009 - PS417_12910 0.37 +1.5 2,825,009 - +1.5 2,825,762 - +0.7 2,825,783 - +0.7 2,825,783 - -0.3 2,826,083 + PS417_12915 0.49 -0.7 2,826,084 - PS417_12915 0.49 -0.1 2,826,289 - PS417_12915 0.82 +0.3 2,826,289 - PS417_12915 0.82 -0.3 2,826,384 - -0.1 2,826,389 + +0.5 2,826,389 + +0.5 2,826,398 + -0.5 2,826,539 + PS417_12920 0.40 -0.2 2,826,539 + PS417_12920 0.40 -0.7 2,826,539 + PS417_12920 0.40 -0.2 2,826,540 - PS417_12920 0.40 +0.6 2,826,540 - PS417_12920 0.40 -0.3 2,826,540 - PS417_12920 0.40 -0.1 2,826,598 + PS417_12920 0.55 +0.0 2,826,689 + PS417_12920 0.79 -0.5 2,826,690 - PS417_12920 0.79 -0.0 2,826,690 - PS417_12920 0.79 +0.4 2,826,690 - PS417_12920 0.79 -0.1 2,826,690 - PS417_12920 0.79 -0.5
Or see this region's nucleotide sequence