Experiment: KB with Curcumin 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3661 and Psyr_3662 overlap by 8 nucleotides Psyr_3662 and Psyr_3663 are separated by 20 nucleotides Psyr_3663 and Psyr_3664 are separated by 100 nucleotides Psyr_3664 and Psyr_3665 are separated by 226 nucleotides
Psyr_3661: Psyr_3661 - Fumarate hydratase, class II, at 4,347,509 to 4,348,903
_3661
Psyr_3662: Psyr_3662 - NAD(P)H dehydrogenase (quinone), at 4,348,896 to 4,349,510
_3662
Psyr_3663: Psyr_3663 - Protein of unknown function DUF6, at 4,349,531 to 4,350,490
_3663
Psyr_3664: Psyr_3664 - DTW, at 4,350,591 to 4,351,190
_3664
Psyr_3665: Psyr_3665 - conserved hypothetical protein, at 4,351,417 to 4,351,719
_3665
Position (kb)
4349
4350
4351 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 4348.583 kb on + strand, within Psyr_3661 at 4348.583 kb on + strand, within Psyr_3661 at 4348.584 kb on - strand, within Psyr_3661 at 4348.584 kb on - strand, within Psyr_3661 at 4348.787 kb on + strand at 4348.788 kb on - strand at 4349.341 kb on - strand, within Psyr_3662 at 4349.356 kb on - strand, within Psyr_3662 at 4349.392 kb on + strand, within Psyr_3662 at 4349.411 kb on - strand, within Psyr_3662 at 4349.411 kb on - strand, within Psyr_3662 at 4349.901 kb on + strand, within Psyr_3663 at 4349.901 kb on + strand, within Psyr_3663 at 4349.915 kb on + strand, within Psyr_3663 at 4349.916 kb on - strand, within Psyr_3663 at 4349.934 kb on - strand, within Psyr_3663 at 4350.015 kb on + strand, within Psyr_3663 at 4350.016 kb on - strand, within Psyr_3663 at 4350.219 kb on + strand, within Psyr_3663 at 4350.220 kb on - strand, within Psyr_3663 at 4350.220 kb on - strand, within Psyr_3663 at 4350.228 kb on + strand, within Psyr_3663 at 4350.228 kb on + strand, within Psyr_3663 at 4350.228 kb on + strand, within Psyr_3663 at 4350.228 kb on + strand, within Psyr_3663 at 4350.228 kb on + strand, within Psyr_3663 at 4350.228 kb on + strand, within Psyr_3663 at 4350.228 kb on + strand, within Psyr_3663 at 4350.228 kb on + strand, within Psyr_3663 at 4350.228 kb on + strand, within Psyr_3663 at 4350.228 kb on + strand, within Psyr_3663 at 4350.228 kb on + strand, within Psyr_3663 at 4350.228 kb on + strand, within Psyr_3663 at 4350.228 kb on + strand, within Psyr_3663 at 4350.229 kb on - strand, within Psyr_3663 at 4350.229 kb on - strand, within Psyr_3663 at 4350.229 kb on - strand, within Psyr_3663 at 4350.229 kb on - strand, within Psyr_3663 at 4350.229 kb on - strand, within Psyr_3663 at 4350.229 kb on - strand, within Psyr_3663 at 4350.229 kb on - strand, within Psyr_3663 at 4350.229 kb on - strand, within Psyr_3663 at 4350.229 kb on - strand, within Psyr_3663 at 4350.229 kb on - strand, within Psyr_3663 at 4350.462 kb on + strand at 4350.463 kb on - strand at 4350.509 kb on + strand at 4350.509 kb on + strand at 4350.509 kb on + strand at 4350.510 kb on - strand at 4350.529 kb on - strand at 4350.554 kb on + strand at 4350.555 kb on - strand at 4350.555 kb on - strand at 4350.555 kb on - strand at 4350.555 kb on - strand at 4350.619 kb on + strand at 4350.619 kb on + strand at 4350.913 kb on + strand, within Psyr_3664 at 4350.914 kb on - strand, within Psyr_3664 at 4350.914 kb on - strand, within Psyr_3664 at 4351.199 kb on - strand at 4351.376 kb on + strand at 4351.377 kb on - strand at 4351.377 kb on - strand at 4351.377 kb on - strand at 4351.377 kb on - strand at 4351.377 kb on - strand at 4351.377 kb on - strand at 4351.387 kb on + strand at 4351.387 kb on + strand at 4351.458 kb on - strand, within Psyr_3665 at 4351.458 kb on - strand, within Psyr_3665 at 4351.469 kb on + strand, within Psyr_3665 at 4351.470 kb on - strand, within Psyr_3665 at 4351.470 kb on - strand, within Psyr_3665 at 4351.470 kb on - strand, within Psyr_3665
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Curcumin 10 mM remove 4,348,583 + Psyr_3661 0.77 +0.8 4,348,583 + Psyr_3661 0.77 -0.5 4,348,584 - Psyr_3661 0.77 +1.3 4,348,584 - Psyr_3661 0.77 +0.4 4,348,787 + +2.1 4,348,788 - -0.4 4,349,341 - Psyr_3662 0.72 +1.2 4,349,356 - Psyr_3662 0.75 +2.6 4,349,392 + Psyr_3662 0.81 -0.1 4,349,411 - Psyr_3662 0.84 +0.0 4,349,411 - Psyr_3662 0.84 -1.1 4,349,901 + Psyr_3663 0.39 -1.0 4,349,901 + Psyr_3663 0.39 +0.7 4,349,915 + Psyr_3663 0.40 -0.7 4,349,916 - Psyr_3663 0.40 +0.2 4,349,934 - Psyr_3663 0.42 +0.0 4,350,015 + Psyr_3663 0.50 +3.5 4,350,016 - Psyr_3663 0.51 +0.4 4,350,219 + Psyr_3663 0.72 +1.7 4,350,220 - Psyr_3663 0.72 -0.0 4,350,220 - Psyr_3663 0.72 +0.2 4,350,228 + Psyr_3663 0.73 +0.6 4,350,228 + Psyr_3663 0.73 -0.2 4,350,228 + Psyr_3663 0.73 -0.4 4,350,228 + Psyr_3663 0.73 +0.4 4,350,228 + Psyr_3663 0.73 +1.0 4,350,228 + Psyr_3663 0.73 +1.7 4,350,228 + Psyr_3663 0.73 -0.5 4,350,228 + Psyr_3663 0.73 +1.2 4,350,228 + Psyr_3663 0.73 +1.4 4,350,228 + Psyr_3663 0.73 +2.4 4,350,228 + Psyr_3663 0.73 -0.2 4,350,228 + Psyr_3663 0.73 +0.0 4,350,228 + Psyr_3663 0.73 -0.3 4,350,229 - Psyr_3663 0.73 -0.0 4,350,229 - Psyr_3663 0.73 -0.3 4,350,229 - Psyr_3663 0.73 +0.6 4,350,229 - Psyr_3663 0.73 -1.1 4,350,229 - Psyr_3663 0.73 +0.7 4,350,229 - Psyr_3663 0.73 -0.5 4,350,229 - Psyr_3663 0.73 +2.5 4,350,229 - Psyr_3663 0.73 -0.3 4,350,229 - Psyr_3663 0.73 -0.3 4,350,229 - Psyr_3663 0.73 -2.7 4,350,462 + +0.7 4,350,463 - -0.7 4,350,509 + +0.0 4,350,509 + -0.0 4,350,509 + +1.0 4,350,510 - -1.6 4,350,529 - +0.8 4,350,554 + -0.0 4,350,555 - +0.2 4,350,555 - -0.4 4,350,555 - +1.1 4,350,555 - +0.5 4,350,619 + -0.1 4,350,619 + +0.4 4,350,913 + Psyr_3664 0.54 +0.4 4,350,914 - Psyr_3664 0.54 +5.3 4,350,914 - Psyr_3664 0.54 +1.0 4,351,199 - -0.1 4,351,376 + -0.0 4,351,377 - +0.9 4,351,377 - +0.7 4,351,377 - +0.8 4,351,377 - -0.5 4,351,377 - -0.2 4,351,377 - +2.9 4,351,387 + +1.1 4,351,387 + -1.2 4,351,458 - Psyr_3665 0.14 -0.8 4,351,458 - Psyr_3665 0.14 -0.4 4,351,469 + Psyr_3665 0.17 -0.4 4,351,470 - Psyr_3665 0.17 -0.3 4,351,470 - Psyr_3665 0.17 -0.4 4,351,470 - Psyr_3665 0.17 +0.4
Or see this region's nucleotide sequence