Experiment: KB with Curcumin 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2269 and Psyr_2270 are separated by 11 nucleotides Psyr_2270 and Psyr_2271 are separated by 140 nucleotides Psyr_2271 and Psyr_2272 are separated by 48 nucleotides Psyr_2272 and Psyr_2273 are separated by 288 nucleotides
Psyr_2269: Psyr_2269 - GTP cyclohydrolase I, at 2,635,090 to 2,635,995
_2269
Psyr_2270: Psyr_2270 - transferase hexapeptide repeat protein, at 2,636,007 to 2,636,567
_2270
Psyr_2271: Psyr_2271 - membrane protein, putative, at 2,636,708 to 2,637,874
_2271
Psyr_2272: Psyr_2272 - Helix-turn-helix motif protein, at 2,637,923 to 2,638,528
_2272
Psyr_2273: Psyr_2273 - L-glutamine synthetase, at 2,638,817 to 2,640,151
_2273
Position (kb)
2636
2637
2638 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 2635.861 kb on + strand, within Psyr_2269 at 2635.861 kb on + strand, within Psyr_2269 at 2635.862 kb on - strand, within Psyr_2269 at 2635.862 kb on - strand, within Psyr_2269 at 2635.984 kb on + strand at 2635.984 kb on + strand at 2635.985 kb on - strand at 2635.985 kb on - strand at 2635.985 kb on - strand at 2635.985 kb on - strand at 2636.058 kb on + strand at 2636.059 kb on - strand at 2636.279 kb on + strand, within Psyr_2270 at 2636.418 kb on + strand, within Psyr_2270 at 2636.418 kb on + strand, within Psyr_2270 at 2636.418 kb on + strand, within Psyr_2270 at 2636.418 kb on + strand, within Psyr_2270 at 2636.418 kb on + strand, within Psyr_2270 at 2636.419 kb on - strand, within Psyr_2270 at 2636.419 kb on - strand, within Psyr_2270 at 2636.419 kb on - strand, within Psyr_2270 at 2636.419 kb on - strand, within Psyr_2270 at 2636.419 kb on - strand, within Psyr_2270 at 2636.419 kb on - strand, within Psyr_2270 at 2636.419 kb on - strand, within Psyr_2270 at 2636.598 kb on + strand at 2636.598 kb on + strand at 2636.599 kb on - strand at 2636.599 kb on - strand at 2636.599 kb on - strand at 2636.599 kb on - strand at 2636.600 kb on + strand at 2636.600 kb on + strand at 2636.600 kb on + strand at 2636.601 kb on - strand at 2636.601 kb on - strand at 2636.612 kb on + strand at 2636.612 kb on + strand at 2636.613 kb on - strand at 2636.620 kb on - strand at 2636.668 kb on + strand at 2636.668 kb on + strand at 2636.669 kb on - strand at 2636.669 kb on - strand at 2636.783 kb on - strand at 2637.080 kb on + strand, within Psyr_2271 at 2637.080 kb on + strand, within Psyr_2271 at 2637.081 kb on - strand, within Psyr_2271 at 2637.081 kb on - strand, within Psyr_2271 at 2637.081 kb on - strand, within Psyr_2271 at 2637.081 kb on - strand, within Psyr_2271 at 2637.640 kb on - strand, within Psyr_2271 at 2637.665 kb on + strand, within Psyr_2271 at 2637.681 kb on - strand, within Psyr_2271 at 2637.698 kb on + strand, within Psyr_2271 at 2637.740 kb on + strand, within Psyr_2271 at 2637.740 kb on + strand, within Psyr_2271 at 2637.741 kb on - strand, within Psyr_2271 at 2637.741 kb on - strand, within Psyr_2271 at 2637.741 kb on - strand, within Psyr_2271 at 2637.787 kb on - strand at 2637.866 kb on + strand at 2637.867 kb on - strand at 2638.065 kb on + strand, within Psyr_2272 at 2638.158 kb on + strand, within Psyr_2272 at 2638.159 kb on - strand, within Psyr_2272 at 2638.159 kb on - strand, within Psyr_2272 at 2638.159 kb on - strand, within Psyr_2272 at 2638.159 kb on - strand, within Psyr_2272 at 2638.159 kb on - strand, within Psyr_2272 at 2638.206 kb on + strand, within Psyr_2272 at 2638.206 kb on + strand, within Psyr_2272 at 2638.206 kb on + strand, within Psyr_2272 at 2638.207 kb on - strand, within Psyr_2272 at 2638.207 kb on - strand, within Psyr_2272 at 2638.482 kb on + strand at 2638.617 kb on - strand at 2638.646 kb on + strand at 2638.646 kb on + strand at 2638.659 kb on + strand at 2638.659 kb on + strand at 2638.660 kb on - strand at 2638.865 kb on + strand at 2638.866 kb on - strand at 2638.866 kb on - strand at 2638.866 kb on - strand at 2638.866 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Curcumin 10 mM remove 2,635,861 + Psyr_2269 0.85 -0.7 2,635,861 + Psyr_2269 0.85 +1.2 2,635,862 - Psyr_2269 0.85 +0.0 2,635,862 - Psyr_2269 0.85 +0.1 2,635,984 + +1.0 2,635,984 + +0.6 2,635,985 - +0.1 2,635,985 - +2.5 2,635,985 - +0.6 2,635,985 - -1.7 2,636,058 + +0.3 2,636,059 - -0.8 2,636,279 + Psyr_2270 0.48 -1.4 2,636,418 + Psyr_2270 0.73 +0.2 2,636,418 + Psyr_2270 0.73 +0.1 2,636,418 + Psyr_2270 0.73 +0.0 2,636,418 + Psyr_2270 0.73 +0.4 2,636,418 + Psyr_2270 0.73 +0.4 2,636,419 - Psyr_2270 0.73 -2.1 2,636,419 - Psyr_2270 0.73 +1.8 2,636,419 - Psyr_2270 0.73 +0.7 2,636,419 - Psyr_2270 0.73 +1.7 2,636,419 - Psyr_2270 0.73 +0.4 2,636,419 - Psyr_2270 0.73 -0.4 2,636,419 - Psyr_2270 0.73 +0.8 2,636,598 + +1.2 2,636,598 + +0.4 2,636,599 - -0.5 2,636,599 - +0.9 2,636,599 - +1.8 2,636,599 - +0.2 2,636,600 + -0.5 2,636,600 + +1.7 2,636,600 + -1.3 2,636,601 - -0.4 2,636,601 - -0.5 2,636,612 + +0.2 2,636,612 + -2.3 2,636,613 - +2.4 2,636,620 - -0.9 2,636,668 + +1.1 2,636,668 + -0.3 2,636,669 - -0.8 2,636,669 - -1.0 2,636,783 - +0.4 2,637,080 + Psyr_2271 0.32 -2.0 2,637,080 + Psyr_2271 0.32 -0.2 2,637,081 - Psyr_2271 0.32 -0.2 2,637,081 - Psyr_2271 0.32 -0.5 2,637,081 - Psyr_2271 0.32 +0.2 2,637,081 - Psyr_2271 0.32 -1.3 2,637,640 - Psyr_2271 0.80 -0.8 2,637,665 + Psyr_2271 0.82 -1.3 2,637,681 - Psyr_2271 0.83 -1.0 2,637,698 + Psyr_2271 0.85 -0.1 2,637,740 + Psyr_2271 0.88 +2.0 2,637,740 + Psyr_2271 0.88 -0.2 2,637,741 - Psyr_2271 0.89 -0.4 2,637,741 - Psyr_2271 0.89 +0.2 2,637,741 - Psyr_2271 0.89 -1.1 2,637,787 - -1.6 2,637,866 + +0.2 2,637,867 - +0.4 2,638,065 + Psyr_2272 0.23 +0.0 2,638,158 + Psyr_2272 0.39 +1.4 2,638,159 - Psyr_2272 0.39 -1.9 2,638,159 - Psyr_2272 0.39 -0.9 2,638,159 - Psyr_2272 0.39 -0.3 2,638,159 - Psyr_2272 0.39 +0.5 2,638,159 - Psyr_2272 0.39 +1.2 2,638,206 + Psyr_2272 0.47 +2.2 2,638,206 + Psyr_2272 0.47 -0.2 2,638,206 + Psyr_2272 0.47 -0.1 2,638,207 - Psyr_2272 0.47 +0.1 2,638,207 - Psyr_2272 0.47 -0.0 2,638,482 + -0.5 2,638,617 - +0.2 2,638,646 + +1.5 2,638,646 + -1.4 2,638,659 + -0.3 2,638,659 + -0.5 2,638,660 - +0.5 2,638,865 + -0.8 2,638,866 - -0.1 2,638,866 - +0.8 2,638,866 - +0.5 2,638,866 - +5.0
Or see this region's nucleotide sequence