Experiment: KB with Curcumin 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1350 and Psyr_1351 are separated by 74 nucleotides Psyr_1351 and Psyr_1352 are separated by 69 nucleotides Psyr_1352 and Psyr_1353 are separated by 2 nucleotides
Psyr_1350: Psyr_1350 - site-2 protease, Metallo peptidase, MEROPS family M50B, at 1,529,409 to 1,530,761
_1350
Psyr_1351: Psyr_1351 - surface antigen (D15):Surface antigen variable number, at 1,530,836 to 1,533,211
_1351
Psyr_1352: Psyr_1352 - Outer membrane protein (OmpH-like), at 1,533,281 to 1,533,760
_1352
Psyr_1353: Psyr_1353 - UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase, at 1,533,763 to 1,534,818
_1353
Position (kb) 1530
1531
1532
1533
1534 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1529.880 kb on - strand, within Psyr_1350 at 1529.880 kb on - strand, within Psyr_1350 at 1529.970 kb on - strand, within Psyr_1350 at 1530.569 kb on + strand, within Psyr_1350 at 1530.569 kb on + strand, within Psyr_1350 at 1530.569 kb on + strand, within Psyr_1350 at 1530.572 kb on + strand, within Psyr_1350 at 1530.572 kb on + strand, within Psyr_1350 at 1530.633 kb on + strand at 1530.712 kb on + strand at 1530.759 kb on + strand at 1530.759 kb on + strand at 1533.209 kb on + strand at 1533.713 kb on + strand at 1533.713 kb on + strand at 1533.713 kb on + strand at 1533.713 kb on + strand at 1533.758 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Curcumin 10 mM remove 1,529,880 - Psyr_1350 0.35 -0.6 1,529,880 - Psyr_1350 0.35 -0.4 1,529,970 - Psyr_1350 0.41 -1.7 1,530,569 + Psyr_1350 0.86 +3.0 1,530,569 + Psyr_1350 0.86 -1.0 1,530,569 + Psyr_1350 0.86 -0.0 1,530,572 + Psyr_1350 0.86 -0.1 1,530,572 + Psyr_1350 0.86 -1.0 1,530,633 + -0.4 1,530,712 + -1.1 1,530,759 + -0.0 1,530,759 + -0.5 1,533,209 + -2.0 1,533,713 + -0.4 1,533,713 + -2.5 1,533,713 + -2.2 1,533,713 + -2.0 1,533,758 + -2.0
Or see this region's nucleotide sequence