Experiment: KB with Curcumin 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0146 and Psyr_0147 are separated by 284 nucleotides Psyr_0147 and Psyr_0148 are separated by 49 nucleotides Psyr_0148 and Psyr_0149 are separated by 77 nucleotides
Psyr_0146: Psyr_0146 - 4-aminobutyrate aminotransferase apoenzyme, at 158,304 to 159,608
_0146
Psyr_0147: Psyr_0147 - D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding protein, at 159,893 to 160,822
_0147
Psyr_0148: Psyr_0148 - hypothetical protein, at 160,872 to 161,111
_0148
Psyr_0149: Psyr_0149 - succinate semialdehyde dehydrogenase, at 161,189 to 162,646
_0149
Position (kb) 159
160
161 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 159.068 kb on - strand, within Psyr_0146 at 159.128 kb on - strand, within Psyr_0146 at 159.128 kb on - strand, within Psyr_0146 at 159.167 kb on + strand, within Psyr_0146 at 159.613 kb on - strand at 159.613 kb on - strand at 159.613 kb on - strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.615 kb on - strand at 159.615 kb on - strand at 159.615 kb on - strand at 159.615 kb on - strand at 159.615 kb on - strand at 159.615 kb on - strand at 159.686 kb on + strand at 159.686 kb on + strand at 159.687 kb on - strand at 159.908 kb on + strand at 159.909 kb on - strand at 159.909 kb on - strand at 159.909 kb on - strand at 160.020 kb on - strand, within Psyr_0147 at 160.124 kb on - strand, within Psyr_0147 at 160.187 kb on + strand, within Psyr_0147 at 160.333 kb on + strand, within Psyr_0147 at 160.333 kb on + strand, within Psyr_0147 at 160.333 kb on + strand, within Psyr_0147 at 160.334 kb on - strand, within Psyr_0147 at 160.415 kb on + strand, within Psyr_0147 at 160.415 kb on + strand, within Psyr_0147 at 160.820 kb on + strand at 160.820 kb on + strand at 160.820 kb on + strand at 160.820 kb on + strand at 160.821 kb on - strand at 160.857 kb on + strand at 160.857 kb on + strand at 160.858 kb on - strand at 161.014 kb on - strand, within Psyr_0148 at 161.014 kb on - strand, within Psyr_0148 at 161.014 kb on - strand, within Psyr_0148 at 161.014 kb on - strand, within Psyr_0148 at 161.068 kb on - strand, within Psyr_0148 at 161.096 kb on + strand at 161.190 kb on + strand at 161.190 kb on + strand at 161.190 kb on + strand at 161.191 kb on - strand at 161.191 kb on - strand at 161.226 kb on + strand at 161.227 kb on - strand at 161.227 kb on - strand at 161.227 kb on - strand at 161.241 kb on + strand at 161.388 kb on + strand, within Psyr_0149 at 161.388 kb on + strand, within Psyr_0149 at 161.388 kb on + strand, within Psyr_0149 at 161.389 kb on - strand, within Psyr_0149 at 161.389 kb on - strand, within Psyr_0149 at 161.389 kb on - strand, within Psyr_0149 at 161.525 kb on + strand, within Psyr_0149 at 161.525 kb on + strand, within Psyr_0149 at 161.526 kb on - strand, within Psyr_0149 at 161.734 kb on - strand, within Psyr_0149 at 161.734 kb on - strand, within Psyr_0149
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Curcumin 10 mM remove 159,068 - Psyr_0146 0.59 -0.8 159,128 - Psyr_0146 0.63 +1.0 159,128 - Psyr_0146 0.63 -1.2 159,167 + Psyr_0146 0.66 -0.2 159,613 - +0.2 159,613 - +0.2 159,613 - +0.7 159,614 + -1.1 159,614 + -0.3 159,614 + +1.8 159,614 + +0.0 159,614 + +0.6 159,614 + -0.5 159,614 + +0.3 159,614 + -0.0 159,614 + -0.3 159,614 + -0.9 159,615 - -0.0 159,615 - -1.1 159,615 - +1.8 159,615 - -0.4 159,615 - -0.4 159,615 - +0.6 159,686 + +0.2 159,686 + +2.0 159,687 - -0.9 159,908 + -0.4 159,909 - +0.9 159,909 - +0.8 159,909 - +3.6 160,020 - Psyr_0147 0.14 +1.1 160,124 - Psyr_0147 0.25 -0.3 160,187 + Psyr_0147 0.32 -0.1 160,333 + Psyr_0147 0.47 -1.2 160,333 + Psyr_0147 0.47 -0.3 160,333 + Psyr_0147 0.47 -0.8 160,334 - Psyr_0147 0.47 +3.3 160,415 + Psyr_0147 0.56 -0.9 160,415 + Psyr_0147 0.56 +0.3 160,820 + -1.2 160,820 + +0.8 160,820 + +0.9 160,820 + -1.0 160,821 - -1.4 160,857 + +0.9 160,857 + -1.6 160,858 - -1.5 161,014 - Psyr_0148 0.59 +0.4 161,014 - Psyr_0148 0.59 -0.2 161,014 - Psyr_0148 0.59 -0.4 161,014 - Psyr_0148 0.59 -0.8 161,068 - Psyr_0148 0.82 -0.7 161,096 + -0.7 161,190 + +0.8 161,190 + -0.3 161,190 + +1.2 161,191 - -0.1 161,191 - -1.2 161,226 + -0.3 161,227 - -1.6 161,227 - +1.2 161,227 - -1.3 161,241 + +0.4 161,388 + Psyr_0149 0.14 -0.6 161,388 + Psyr_0149 0.14 -3.8 161,388 + Psyr_0149 0.14 +0.1 161,389 - Psyr_0149 0.14 +0.0 161,389 - Psyr_0149 0.14 +0.0 161,389 - Psyr_0149 0.14 -2.8 161,525 + Psyr_0149 0.23 -0.5 161,525 + Psyr_0149 0.23 +0.2 161,526 - Psyr_0149 0.23 -1.3 161,734 - Psyr_0149 0.37 +0.5 161,734 - Psyr_0149 0.37 +0.3
Or see this region's nucleotide sequence