Experiment: Lactitol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3349 and Atu3350 are separated by 5 nucleotides Atu3350 and Atu3351 overlap by 8 nucleotides Atu3351 and Atu3352 are separated by 143 nucleotides
Atu3349: Atu3349 - ABC transporter permease, at 382,523 to 383,611
Atu3349
Atu3350: Atu3350 - ABC transporter permease, at 383,617 to 384,558
Atu3350
Atu3351: Atu3351 - sugar ABC transporter permease, at 384,551 to 385,453
Atu3351
Atu3352: Atu3352 - sugar ABC transporter substrate-binding protein, at 385,597 to 386,829
Atu3352
Position (kb) 384
385
386 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 383.572 kb on + strand at 383.688 kb on + strand at 383.745 kb on + strand, within Atu3350 at 383.800 kb on + strand, within Atu3350 at 383.808 kb on - strand, within Atu3350 at 383.852 kb on - strand, within Atu3350 at 383.882 kb on - strand, within Atu3350 at 383.932 kb on - strand, within Atu3350 at 383.947 kb on - strand, within Atu3350 at 384.109 kb on - strand, within Atu3350 at 384.126 kb on - strand, within Atu3350 at 384.138 kb on + strand, within Atu3350 at 384.146 kb on - strand, within Atu3350 at 384.158 kb on + strand, within Atu3350 at 384.265 kb on + strand, within Atu3350 at 384.265 kb on + strand, within Atu3350 at 384.273 kb on - strand, within Atu3350 at 384.273 kb on - strand, within Atu3350 at 384.287 kb on + strand, within Atu3350 at 384.294 kb on + strand, within Atu3350 at 384.294 kb on + strand, within Atu3350 at 384.300 kb on - strand, within Atu3350 at 384.302 kb on - strand, within Atu3350 at 384.302 kb on - strand, within Atu3350 at 384.306 kb on + strand, within Atu3350 at 384.314 kb on - strand, within Atu3350 at 384.419 kb on + strand, within Atu3350 at 384.427 kb on - strand, within Atu3350 at 384.626 kb on + strand at 384.636 kb on + strand at 384.641 kb on + strand at 384.644 kb on - strand, within Atu3351 at 384.646 kb on + strand, within Atu3351 at 384.720 kb on + strand, within Atu3351 at 384.773 kb on + strand, within Atu3351 at 384.823 kb on + strand, within Atu3351 at 384.823 kb on + strand, within Atu3351 at 384.823 kb on + strand, within Atu3351 at 384.831 kb on - strand, within Atu3351 at 384.928 kb on + strand, within Atu3351 at 384.955 kb on - strand, within Atu3351 at 384.973 kb on + strand, within Atu3351 at 385.231 kb on + strand, within Atu3351 at 385.239 kb on - strand, within Atu3351 at 385.246 kb on + strand, within Atu3351 at 385.249 kb on - strand, within Atu3351 at 385.268 kb on - strand, within Atu3351 at 385.268 kb on - strand, within Atu3351 at 385.340 kb on - strand, within Atu3351 at 385.381 kb on - strand at 385.438 kb on + strand at 385.446 kb on - strand at 385.525 kb on - strand at 385.549 kb on - strand at 385.724 kb on + strand, within Atu3352 at 385.724 kb on + strand, within Atu3352 at 385.727 kb on - strand, within Atu3352 at 385.732 kb on - strand, within Atu3352 at 385.737 kb on - strand, within Atu3352 at 385.744 kb on - strand, within Atu3352 at 385.750 kb on - strand, within Atu3352 at 385.785 kb on - strand, within Atu3352 at 385.785 kb on - strand, within Atu3352 at 385.795 kb on - strand, within Atu3352 at 385.795 kb on - strand, within Atu3352 at 385.891 kb on - strand, within Atu3352 at 385.981 kb on + strand, within Atu3352 at 385.981 kb on + strand, within Atu3352 at 386.039 kb on + strand, within Atu3352 at 386.047 kb on - strand, within Atu3352 at 386.047 kb on - strand, within Atu3352 at 386.047 kb on - strand, within Atu3352 at 386.047 kb on - strand, within Atu3352 at 386.355 kb on + strand, within Atu3352
Per-strain Table
Position Strand Gene LocusTag Fraction Lactitol (C) remove 383,572 + -3.0 383,688 + -1.8 383,745 + Atu3350 0.14 -1.6 383,800 + Atu3350 0.19 -3.5 383,808 - Atu3350 0.20 -1.6 383,852 - Atu3350 0.25 -2.7 383,882 - Atu3350 0.28 -1.6 383,932 - Atu3350 0.33 -3.5 383,947 - Atu3350 0.35 -2.6 384,109 - Atu3350 0.52 -2.3 384,126 - Atu3350 0.54 -3.8 384,138 + Atu3350 0.55 -2.7 384,146 - Atu3350 0.56 -4.6 384,158 + Atu3350 0.57 -3.6 384,265 + Atu3350 0.69 -2.2 384,265 + Atu3350 0.69 -3.9 384,273 - Atu3350 0.70 -3.3 384,273 - Atu3350 0.70 -2.2 384,287 + Atu3350 0.71 -2.4 384,294 + Atu3350 0.72 -1.8 384,294 + Atu3350 0.72 -3.8 384,300 - Atu3350 0.73 -2.1 384,302 - Atu3350 0.73 -0.4 384,302 - Atu3350 0.73 -4.7 384,306 + Atu3350 0.73 -4.1 384,314 - Atu3350 0.74 -3.8 384,419 + Atu3350 0.85 -2.3 384,427 - Atu3350 0.86 +1.4 384,626 + -2.2 384,636 + -2.0 384,641 + -1.7 384,644 - Atu3351 0.10 -2.6 384,646 + Atu3351 0.11 -3.1 384,720 + Atu3351 0.19 -2.0 384,773 + Atu3351 0.25 -3.0 384,823 + Atu3351 0.30 -3.5 384,823 + Atu3351 0.30 -2.2 384,823 + Atu3351 0.30 -3.5 384,831 - Atu3351 0.31 -2.2 384,928 + Atu3351 0.42 -2.9 384,955 - Atu3351 0.45 -2.8 384,973 + Atu3351 0.47 -2.7 385,231 + Atu3351 0.75 -4.0 385,239 - Atu3351 0.76 -3.3 385,246 + Atu3351 0.77 -4.4 385,249 - Atu3351 0.77 -3.9 385,268 - Atu3351 0.79 -5.3 385,268 - Atu3351 0.79 -2.4 385,340 - Atu3351 0.87 -2.2 385,381 - -4.4 385,438 + -2.5 385,446 - -4.8 385,525 - -1.8 385,549 - -0.7 385,724 + Atu3352 0.10 -3.0 385,724 + Atu3352 0.10 -2.7 385,727 - Atu3352 0.11 -3.5 385,732 - Atu3352 0.11 -3.7 385,737 - Atu3352 0.11 -4.7 385,744 - Atu3352 0.12 -3.7 385,750 - Atu3352 0.12 -1.8 385,785 - Atu3352 0.15 -1.8 385,785 - Atu3352 0.15 -2.0 385,795 - Atu3352 0.16 -3.6 385,795 - Atu3352 0.16 -4.0 385,891 - Atu3352 0.24 -4.2 385,981 + Atu3352 0.31 -3.1 385,981 + Atu3352 0.31 -4.3 386,039 + Atu3352 0.36 -5.5 386,047 - Atu3352 0.36 -1.2 386,047 - Atu3352 0.36 -3.6 386,047 - Atu3352 0.36 -3.0 386,047 - Atu3352 0.36 -3.4 386,355 + Atu3352 0.61 -4.4
Or see this region's nucleotide sequence