Experiment: RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11300 and PS417_11305 overlap by 1 nucleotides PS417_11305 and PS417_11310 are separated by 329 nucleotides
PS417_11300: PS417_11300 - general secretion pathway protein GspE, at 2,419,461 to 2,420,879
_11300
PS417_11305: PS417_11305 - general secretion pathway protein GspF, at 2,420,879 to 2,422,090
_11305
PS417_11310: PS417_11310 - alkaline phosphatase, at 2,422,420 to 2,423,601
_11310
Position (kb)
2420
2421
2422
2423 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2420.230 kb on - strand, within PS417_11300 at 2420.894 kb on + strand at 2421.269 kb on + strand, within PS417_11305 at 2421.339 kb on - strand, within PS417_11305 at 2421.443 kb on + strand, within PS417_11305 at 2421.444 kb on - strand, within PS417_11305 at 2421.444 kb on - strand, within PS417_11305 at 2421.444 kb on - strand, within PS417_11305 at 2421.597 kb on - strand, within PS417_11305 at 2422.072 kb on - strand at 2422.089 kb on - strand at 2422.118 kb on + strand at 2422.119 kb on - strand at 2422.305 kb on + strand at 2422.393 kb on + strand at 2422.547 kb on + strand, within PS417_11310 at 2422.548 kb on - strand, within PS417_11310 at 2422.621 kb on + strand, within PS417_11310 at 2422.708 kb on + strand, within PS417_11310 at 2422.709 kb on - strand, within PS417_11310
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 2,420,230 - PS417_11300 0.54 -0.6 2,420,894 + +1.1 2,421,269 + PS417_11305 0.32 -0.7 2,421,339 - PS417_11305 0.38 -0.8 2,421,443 + PS417_11305 0.47 -3.2 2,421,444 - PS417_11305 0.47 -0.5 2,421,444 - PS417_11305 0.47 -0.7 2,421,444 - PS417_11305 0.47 +2.3 2,421,597 - PS417_11305 0.59 -1.2 2,422,072 - +0.6 2,422,089 - +0.7 2,422,118 + +0.1 2,422,119 - -0.2 2,422,305 + +0.7 2,422,393 + +0.1 2,422,547 + PS417_11310 0.11 -0.1 2,422,548 - PS417_11310 0.11 -1.0 2,422,621 + PS417_11310 0.17 +0.6 2,422,708 + PS417_11310 0.24 +1.0 2,422,709 - PS417_11310 0.24 +0.6
Or see this region's nucleotide sequence