Experiment: KB with Veratrine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4270 and Psyr_4271 are separated by 104 nucleotides Psyr_4271 and Psyr_4272 are separated by 95 nucleotides Psyr_4272 and Psyr_4273 are separated by 27 nucleotides
Psyr_4270: Psyr_4270 - serine hydroxymethyltransferase, at 5,094,122 to 5,095,375
_4270
Psyr_4271: Psyr_4271 - Cobalamin synthesis protein/P47K:Cobalamin synthesis protein/P47K, at 5,095,480 to 5,096,457
_4271
Psyr_4272: Psyr_4272 - Protein of unknown function DUF466, at 5,096,553 to 5,096,750
_4272
Psyr_4273: Psyr_4273 - Carbon starvation protein CstA, at 5,096,778 to 5,098,853
_4273
Position (kb) 5095
5096
5097 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5094.539 kb on + strand, within Psyr_4270 at 5094.540 kb on - strand, within Psyr_4270 at 5094.585 kb on - strand, within Psyr_4270 at 5094.651 kb on - strand, within Psyr_4270 at 5094.708 kb on - strand, within Psyr_4270 at 5094.708 kb on - strand, within Psyr_4270 at 5094.708 kb on - strand, within Psyr_4270 at 5094.977 kb on + strand, within Psyr_4270 at 5095.388 kb on - strand at 5095.388 kb on - strand at 5095.396 kb on + strand at 5095.489 kb on + strand at 5095.489 kb on + strand at 5095.489 kb on + strand at 5095.490 kb on - strand at 5095.490 kb on - strand at 5095.490 kb on - strand at 5095.667 kb on + strand, within Psyr_4271 at 5095.668 kb on - strand, within Psyr_4271 at 5096.087 kb on + strand, within Psyr_4271 at 5096.088 kb on - strand, within Psyr_4271 at 5096.088 kb on - strand, within Psyr_4271 at 5096.088 kb on - strand, within Psyr_4271 at 5096.088 kb on - strand, within Psyr_4271 at 5096.088 kb on - strand, within Psyr_4271 at 5096.199 kb on + strand, within Psyr_4271 at 5096.199 kb on + strand, within Psyr_4271 at 5096.217 kb on - strand, within Psyr_4271 at 5096.217 kb on - strand, within Psyr_4271 at 5096.217 kb on - strand, within Psyr_4271 at 5096.284 kb on + strand, within Psyr_4271 at 5096.284 kb on + strand, within Psyr_4271 at 5096.457 kb on + strand at 5096.457 kb on + strand at 5096.458 kb on - strand at 5096.536 kb on + strand at 5096.620 kb on + strand, within Psyr_4272 at 5096.620 kb on + strand, within Psyr_4272 at 5096.620 kb on + strand, within Psyr_4272 at 5096.620 kb on + strand, within Psyr_4272 at 5096.621 kb on - strand, within Psyr_4272 at 5096.668 kb on + strand, within Psyr_4272 at 5096.668 kb on + strand, within Psyr_4272 at 5096.669 kb on - strand, within Psyr_4272 at 5096.669 kb on - strand, within Psyr_4272 at 5096.669 kb on - strand, within Psyr_4272 at 5096.669 kb on - strand, within Psyr_4272 at 5096.719 kb on + strand, within Psyr_4272 at 5096.720 kb on - strand, within Psyr_4272 at 5096.865 kb on - strand at 5096.872 kb on + strand at 5096.872 kb on + strand at 5096.873 kb on - strand at 5096.929 kb on + strand at 5096.929 kb on + strand at 5096.930 kb on - strand at 5097.010 kb on + strand, within Psyr_4273 at 5097.010 kb on + strand, within Psyr_4273 at 5097.010 kb on + strand, within Psyr_4273 at 5097.011 kb on - strand, within Psyr_4273 at 5097.011 kb on - strand, within Psyr_4273 at 5097.011 kb on - strand, within Psyr_4273 at 5097.036 kb on + strand, within Psyr_4273 at 5097.128 kb on - strand, within Psyr_4273 at 5097.203 kb on + strand, within Psyr_4273 at 5097.220 kb on - strand, within Psyr_4273 at 5097.220 kb on - strand, within Psyr_4273 at 5097.224 kb on + strand, within Psyr_4273 at 5097.224 kb on + strand, within Psyr_4273 at 5097.224 kb on + strand, within Psyr_4273 at 5097.224 kb on + strand, within Psyr_4273 at 5097.224 kb on + strand, within Psyr_4273 at 5097.224 kb on + strand, within Psyr_4273 at 5097.225 kb on - strand, within Psyr_4273 at 5097.225 kb on - strand, within Psyr_4273 at 5097.225 kb on - strand, within Psyr_4273 at 5097.268 kb on + strand, within Psyr_4273
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Veratrine 10 mM remove 5,094,539 + Psyr_4270 0.33 -1.7 5,094,540 - Psyr_4270 0.33 -0.9 5,094,585 - Psyr_4270 0.37 +0.4 5,094,651 - Psyr_4270 0.42 +1.9 5,094,708 - Psyr_4270 0.47 +0.8 5,094,708 - Psyr_4270 0.47 -0.5 5,094,708 - Psyr_4270 0.47 +0.5 5,094,977 + Psyr_4270 0.68 -1.6 5,095,388 - +0.0 5,095,388 - +0.3 5,095,396 + -0.4 5,095,489 + -0.1 5,095,489 + -0.4 5,095,489 + +0.2 5,095,490 - +0.2 5,095,490 - -0.9 5,095,490 - +0.3 5,095,667 + Psyr_4271 0.19 +0.4 5,095,668 - Psyr_4271 0.19 +0.5 5,096,087 + Psyr_4271 0.62 -0.9 5,096,088 - Psyr_4271 0.62 +0.5 5,096,088 - Psyr_4271 0.62 -1.7 5,096,088 - Psyr_4271 0.62 -0.4 5,096,088 - Psyr_4271 0.62 +1.1 5,096,088 - Psyr_4271 0.62 +0.1 5,096,199 + Psyr_4271 0.74 -0.8 5,096,199 + Psyr_4271 0.74 -0.2 5,096,217 - Psyr_4271 0.75 +0.7 5,096,217 - Psyr_4271 0.75 +0.7 5,096,217 - Psyr_4271 0.75 -0.9 5,096,284 + Psyr_4271 0.82 +0.9 5,096,284 + Psyr_4271 0.82 +0.4 5,096,457 + +0.1 5,096,457 + -1.0 5,096,458 - +1.7 5,096,536 + -0.8 5,096,620 + Psyr_4272 0.34 -1.5 5,096,620 + Psyr_4272 0.34 -0.5 5,096,620 + Psyr_4272 0.34 +1.1 5,096,620 + Psyr_4272 0.34 +0.4 5,096,621 - Psyr_4272 0.34 -1.0 5,096,668 + Psyr_4272 0.58 +2.3 5,096,668 + Psyr_4272 0.58 +1.1 5,096,669 - Psyr_4272 0.59 -1.4 5,096,669 - Psyr_4272 0.59 -0.0 5,096,669 - Psyr_4272 0.59 +1.6 5,096,669 - Psyr_4272 0.59 -1.6 5,096,719 + Psyr_4272 0.84 -3.3 5,096,720 - Psyr_4272 0.84 +1.5 5,096,865 - +1.3 5,096,872 + -0.0 5,096,872 + -0.0 5,096,873 - -0.5 5,096,929 + -1.7 5,096,929 + -2.2 5,096,930 - +0.5 5,097,010 + Psyr_4273 0.11 -1.0 5,097,010 + Psyr_4273 0.11 -0.7 5,097,010 + Psyr_4273 0.11 +1.2 5,097,011 - Psyr_4273 0.11 +0.5 5,097,011 - Psyr_4273 0.11 +1.3 5,097,011 - Psyr_4273 0.11 -0.7 5,097,036 + Psyr_4273 0.12 -0.9 5,097,128 - Psyr_4273 0.17 +1.3 5,097,203 + Psyr_4273 0.20 +2.1 5,097,220 - Psyr_4273 0.21 -0.8 5,097,220 - Psyr_4273 0.21 +0.0 5,097,224 + Psyr_4273 0.21 +2.4 5,097,224 + Psyr_4273 0.21 +0.5 5,097,224 + Psyr_4273 0.21 -0.4 5,097,224 + Psyr_4273 0.21 -1.5 5,097,224 + Psyr_4273 0.21 -1.0 5,097,224 + Psyr_4273 0.21 -0.3 5,097,225 - Psyr_4273 0.22 +2.1 5,097,225 - Psyr_4273 0.22 +1.8 5,097,225 - Psyr_4273 0.22 +0.8 5,097,268 + Psyr_4273 0.24 -1.3
Or see this region's nucleotide sequence