Experiment: KB with Veratrine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2226 and Psyr_2227 are separated by 297 nucleotides Psyr_2227 and Psyr_2228 are separated by 399 nucleotides
Psyr_2226: Psyr_2226 - formyltetrahydrofolate deformylase, at 2,587,752 to 2,588,618
_2226
Psyr_2227: Psyr_2227 - conserved hypothetical protein, at 2,588,916 to 2,589,371
_2227
Psyr_2228: Psyr_2228 - conserved hypothetical protein, at 2,589,771 to 2,590,499
_2228
Position (kb)
2588
2589
2590 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2587.993 kb on + strand, within Psyr_2226 at 2588.037 kb on - strand, within Psyr_2226 at 2588.093 kb on - strand, within Psyr_2226 at 2588.093 kb on - strand, within Psyr_2226 at 2588.290 kb on + strand, within Psyr_2226 at 2588.518 kb on + strand, within Psyr_2226 at 2588.518 kb on + strand, within Psyr_2226 at 2588.518 kb on + strand, within Psyr_2226 at 2588.518 kb on + strand, within Psyr_2226 at 2588.519 kb on - strand, within Psyr_2226 at 2588.519 kb on - strand, within Psyr_2226 at 2588.519 kb on - strand, within Psyr_2226 at 2588.519 kb on - strand, within Psyr_2226 at 2588.519 kb on - strand, within Psyr_2226 at 2588.540 kb on - strand at 2588.737 kb on - strand at 2588.737 kb on - strand at 2588.737 kb on - strand at 2588.737 kb on - strand at 2588.888 kb on + strand at 2588.888 kb on + strand at 2588.889 kb on - strand at 2588.889 kb on - strand at 2588.889 kb on - strand at 2588.917 kb on + strand at 2588.918 kb on - strand at 2588.918 kb on - strand at 2588.966 kb on + strand, within Psyr_2227 at 2589.332 kb on + strand at 2589.333 kb on - strand at 2589.409 kb on + strand at 2589.410 kb on - strand at 2589.453 kb on + strand at 2589.454 kb on - strand at 2589.458 kb on - strand at 2589.474 kb on + strand at 2589.474 kb on + strand at 2589.475 kb on - strand at 2589.476 kb on + strand at 2589.521 kb on + strand at 2589.522 kb on - strand at 2589.729 kb on - strand at 2589.810 kb on - strand at 2590.111 kb on + strand, within Psyr_2228 at 2590.111 kb on + strand, within Psyr_2228 at 2590.112 kb on - strand, within Psyr_2228 at 2590.112 kb on - strand, within Psyr_2228 at 2590.112 kb on - strand, within Psyr_2228 at 2590.137 kb on - strand, within Psyr_2228
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Veratrine 10 mM remove 2,587,993 + Psyr_2226 0.28 -2.5 2,588,037 - Psyr_2226 0.33 +1.3 2,588,093 - Psyr_2226 0.39 +1.1 2,588,093 - Psyr_2226 0.39 -1.1 2,588,290 + Psyr_2226 0.62 -0.9 2,588,518 + Psyr_2226 0.88 -0.2 2,588,518 + Psyr_2226 0.88 -2.9 2,588,518 + Psyr_2226 0.88 -0.2 2,588,518 + Psyr_2226 0.88 +0.8 2,588,519 - Psyr_2226 0.88 -0.5 2,588,519 - Psyr_2226 0.88 -0.7 2,588,519 - Psyr_2226 0.88 -0.3 2,588,519 - Psyr_2226 0.88 -0.3 2,588,519 - Psyr_2226 0.88 -1.8 2,588,540 - +1.1 2,588,737 - +0.7 2,588,737 - +0.1 2,588,737 - -0.7 2,588,737 - +0.2 2,588,888 + -2.2 2,588,888 + +0.2 2,588,889 - -0.0 2,588,889 - -0.5 2,588,889 - -1.0 2,588,917 + -2.7 2,588,918 - -1.5 2,588,918 - -0.2 2,588,966 + Psyr_2227 0.11 -2.2 2,589,332 + +1.2 2,589,333 - -2.2 2,589,409 + -0.2 2,589,410 - +0.5 2,589,453 + -1.0 2,589,454 - -1.1 2,589,458 - +1.3 2,589,474 + +4.6 2,589,474 + +0.0 2,589,475 - -1.7 2,589,476 + -1.5 2,589,521 + -0.8 2,589,522 - +0.7 2,589,729 - -1.0 2,589,810 - -1.1 2,590,111 + Psyr_2228 0.47 -1.5 2,590,111 + Psyr_2228 0.47 -0.7 2,590,112 - Psyr_2228 0.47 +0.1 2,590,112 - Psyr_2228 0.47 -1.1 2,590,112 - Psyr_2228 0.47 -0.0 2,590,137 - Psyr_2228 0.50 -0.6
Or see this region's nucleotide sequence