Experiment: KB with Harmol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3024 and Psyr_3025 are separated by 36 nucleotides Psyr_3025 and Psyr_3026 are separated by 106 nucleotides Psyr_3026 and Psyr_3027 are separated by 78 nucleotides
Psyr_3024: Psyr_3024 - Peptidase M14, carboxypeptidase A, at 3,621,563 to 3,622,699
_3024
Psyr_3025: Psyr_3025 - Major facilitator superfamily, at 3,622,736 to 3,623,965
_3025
Psyr_3026: Psyr_3026 - transcriptional regulator, TetR family, at 3,624,072 to 3,624,617
_3026
Psyr_3027: Psyr_3027 - Protein kinase:Leucine-rich repeat protein, at 3,624,696 to 3,626,000
_3027
Position (kb)
3622
3623
3624 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3621.780 kb on - strand, within Psyr_3024 at 3621.906 kb on - strand, within Psyr_3024 at 3622.001 kb on - strand, within Psyr_3024 at 3622.173 kb on - strand, within Psyr_3024 at 3622.367 kb on + strand, within Psyr_3024 at 3622.371 kb on + strand, within Psyr_3024 at 3622.371 kb on + strand, within Psyr_3024 at 3622.372 kb on - strand, within Psyr_3024 at 3622.372 kb on - strand, within Psyr_3024 at 3622.372 kb on - strand, within Psyr_3024 at 3622.372 kb on - strand, within Psyr_3024 at 3622.403 kb on + strand, within Psyr_3024 at 3622.550 kb on + strand, within Psyr_3024 at 3622.551 kb on - strand, within Psyr_3024 at 3622.659 kb on + strand at 3622.659 kb on + strand at 3622.659 kb on + strand at 3622.659 kb on + strand at 3622.659 kb on + strand at 3622.659 kb on + strand at 3622.659 kb on + strand at 3622.660 kb on - strand at 3622.660 kb on - strand at 3622.660 kb on - strand at 3622.660 kb on - strand at 3622.660 kb on - strand at 3622.683 kb on + strand at 3622.683 kb on + strand at 3622.684 kb on - strand at 3622.738 kb on - strand at 3622.906 kb on - strand, within Psyr_3025 at 3622.906 kb on - strand, within Psyr_3025 at 3623.165 kb on + strand, within Psyr_3025 at 3623.165 kb on + strand, within Psyr_3025 at 3623.303 kb on + strand, within Psyr_3025 at 3623.303 kb on + strand, within Psyr_3025 at 3623.303 kb on + strand, within Psyr_3025 at 3623.304 kb on - strand, within Psyr_3025 at 3623.304 kb on - strand, within Psyr_3025 at 3623.400 kb on + strand, within Psyr_3025 at 3623.524 kb on + strand, within Psyr_3025 at 3623.524 kb on + strand, within Psyr_3025 at 3623.524 kb on + strand, within Psyr_3025 at 3623.525 kb on - strand, within Psyr_3025 at 3623.525 kb on - strand, within Psyr_3025 at 3623.721 kb on + strand, within Psyr_3025 at 3623.772 kb on + strand, within Psyr_3025 at 3623.772 kb on + strand, within Psyr_3025 at 3623.772 kb on + strand, within Psyr_3025 at 3623.773 kb on - strand, within Psyr_3025 at 3623.773 kb on - strand, within Psyr_3025 at 3623.773 kb on - strand, within Psyr_3025 at 3623.773 kb on - strand, within Psyr_3025 at 3623.773 kb on - strand, within Psyr_3025 at 3623.774 kb on + strand, within Psyr_3025 at 3623.775 kb on - strand, within Psyr_3025 at 3623.775 kb on - strand, within Psyr_3025 at 3623.775 kb on - strand, within Psyr_3025 at 3623.827 kb on - strand, within Psyr_3025 at 3623.835 kb on + strand, within Psyr_3025 at 3623.836 kb on - strand, within Psyr_3025 at 3623.873 kb on + strand at 3623.970 kb on - strand at 3624.225 kb on - strand, within Psyr_3026 at 3624.225 kb on - strand, within Psyr_3026 at 3624.225 kb on - strand, within Psyr_3026 at 3624.349 kb on - strand, within Psyr_3026 at 3624.349 kb on - strand, within Psyr_3026 at 3624.349 kb on - strand, within Psyr_3026 at 3624.691 kb on + strand at 3624.691 kb on + strand at 3624.691 kb on + strand at 3624.691 kb on + strand at 3624.692 kb on - strand at 3624.692 kb on - strand at 3624.715 kb on + strand at 3624.716 kb on - strand at 3624.909 kb on - strand, within Psyr_3027
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmol 10 mM remove 3,621,780 - Psyr_3024 0.19 +0.4 3,621,906 - Psyr_3024 0.30 +0.4 3,622,001 - Psyr_3024 0.39 -0.5 3,622,173 - Psyr_3024 0.54 -1.5 3,622,367 + Psyr_3024 0.71 -0.8 3,622,371 + Psyr_3024 0.71 +0.3 3,622,371 + Psyr_3024 0.71 +1.0 3,622,372 - Psyr_3024 0.71 -0.8 3,622,372 - Psyr_3024 0.71 +0.4 3,622,372 - Psyr_3024 0.71 +1.4 3,622,372 - Psyr_3024 0.71 -0.6 3,622,403 + Psyr_3024 0.74 -1.6 3,622,550 + Psyr_3024 0.87 -0.9 3,622,551 - Psyr_3024 0.87 +0.0 3,622,659 + +1.2 3,622,659 + -0.1 3,622,659 + +3.3 3,622,659 + -0.6 3,622,659 + +2.4 3,622,659 + +0.5 3,622,659 + +3.0 3,622,660 - +1.9 3,622,660 - +0.3 3,622,660 - -0.4 3,622,660 - +0.1 3,622,660 - -0.1 3,622,683 + -2.2 3,622,683 + +1.2 3,622,684 - +0.7 3,622,738 - +0.2 3,622,906 - Psyr_3025 0.14 -0.8 3,622,906 - Psyr_3025 0.14 +0.3 3,623,165 + Psyr_3025 0.35 -1.6 3,623,165 + Psyr_3025 0.35 +0.6 3,623,303 + Psyr_3025 0.46 -1.5 3,623,303 + Psyr_3025 0.46 -0.3 3,623,303 + Psyr_3025 0.46 -0.1 3,623,304 - Psyr_3025 0.46 +0.2 3,623,304 - Psyr_3025 0.46 +3.0 3,623,400 + Psyr_3025 0.54 -0.1 3,623,524 + Psyr_3025 0.64 -2.8 3,623,524 + Psyr_3025 0.64 +1.4 3,623,524 + Psyr_3025 0.64 -1.6 3,623,525 - Psyr_3025 0.64 +0.5 3,623,525 - Psyr_3025 0.64 +1.2 3,623,721 + Psyr_3025 0.80 +0.1 3,623,772 + Psyr_3025 0.84 -0.1 3,623,772 + Psyr_3025 0.84 +1.4 3,623,772 + Psyr_3025 0.84 +0.1 3,623,773 - Psyr_3025 0.84 +0.8 3,623,773 - Psyr_3025 0.84 +0.4 3,623,773 - Psyr_3025 0.84 +1.8 3,623,773 - Psyr_3025 0.84 +0.4 3,623,773 - Psyr_3025 0.84 -1.3 3,623,774 + Psyr_3025 0.84 -0.6 3,623,775 - Psyr_3025 0.84 -2.3 3,623,775 - Psyr_3025 0.84 -0.8 3,623,775 - Psyr_3025 0.84 +0.5 3,623,827 - Psyr_3025 0.89 +0.6 3,623,835 + Psyr_3025 0.89 +1.4 3,623,836 - Psyr_3025 0.89 +0.4 3,623,873 + +0.2 3,623,970 - +0.7 3,624,225 - Psyr_3026 0.28 +3.4 3,624,225 - Psyr_3026 0.28 +0.4 3,624,225 - Psyr_3026 0.28 +2.3 3,624,349 - Psyr_3026 0.51 -3.2 3,624,349 - Psyr_3026 0.51 +0.3 3,624,349 - Psyr_3026 0.51 -0.8 3,624,691 + -0.6 3,624,691 + +2.2 3,624,691 + -0.8 3,624,691 + +0.1 3,624,692 - -2.1 3,624,692 - +1.4 3,624,715 + -1.1 3,624,716 - -1.1 3,624,909 - Psyr_3027 0.16 -1.6
Or see this region's nucleotide sequence