Experiment: KB with Harmol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2667 and Psyr_2668 are separated by 125 nucleotides Psyr_2668 and Psyr_2669 are separated by 65 nucleotides Psyr_2669 and Psyr_2670 are separated by 5 nucleotides
Psyr_2667: Psyr_2667 - Transposase Tn3, at 3,236,917 to 3,239,802
_2667
Psyr_2668: Psyr_2668 - Helix-turn-helix, Fis-type, at 3,239,928 to 3,240,542
_2668
Psyr_2669: Psyr_2669 - Aminoglycoside phosphotransferase, at 3,240,608 to 3,241,411
_2669
Psyr_2670: Psyr_2670 - Aminoglycoside/hydroxyurea antibiotic resistance kinase, at 3,241,417 to 3,242,247
_2670
Position (kb) 3239
3240
3241 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 3239.018 kb on + strand, within Psyr_2667 at 3239.021 kb on + strand, within Psyr_2667 at 3239.108 kb on + strand, within Psyr_2667 at 3239.245 kb on - strand, within Psyr_2667 at 3239.245 kb on - strand, within Psyr_2667 at 3239.382 kb on + strand, within Psyr_2667 at 3239.483 kb on - strand, within Psyr_2667 at 3239.525 kb on + strand at 3239.525 kb on + strand at 3239.525 kb on + strand at 3239.526 kb on - strand at 3239.526 kb on - strand at 3239.526 kb on - strand at 3239.531 kb on - strand at 3239.569 kb on - strand at 3239.688 kb on + strand at 3239.689 kb on - strand at 3239.689 kb on - strand at 3239.715 kb on + strand at 3239.715 kb on + strand at 3239.715 kb on + strand at 3239.716 kb on - strand at 3239.716 kb on - strand at 3239.718 kb on - strand at 3239.718 kb on - strand at 3239.718 kb on - strand at 3239.802 kb on + strand at 3239.848 kb on + strand at 3239.848 kb on + strand at 3239.940 kb on + strand at 3240.026 kb on + strand, within Psyr_2668 at 3240.026 kb on + strand, within Psyr_2668 at 3240.026 kb on + strand, within Psyr_2668 at 3240.026 kb on + strand, within Psyr_2668 at 3240.030 kb on + strand, within Psyr_2668 at 3240.030 kb on + strand, within Psyr_2668 at 3240.030 kb on + strand, within Psyr_2668 at 3240.030 kb on + strand, within Psyr_2668 at 3240.030 kb on + strand, within Psyr_2668 at 3240.030 kb on + strand, within Psyr_2668 at 3240.030 kb on + strand, within Psyr_2668 at 3240.030 kb on + strand, within Psyr_2668 at 3240.030 kb on + strand, within Psyr_2668 at 3240.030 kb on + strand, within Psyr_2668 at 3240.426 kb on - strand, within Psyr_2668 at 3240.484 kb on - strand at 3240.536 kb on + strand at 3240.536 kb on + strand at 3240.536 kb on + strand at 3240.536 kb on + strand at 3240.536 kb on + strand at 3240.536 kb on + strand at 3240.536 kb on + strand at 3240.537 kb on - strand at 3240.537 kb on - strand at 3240.540 kb on + strand at 3240.541 kb on - strand at 3241.329 kb on + strand, within Psyr_2669 at 3241.338 kb on + strand at 3241.338 kb on + strand at 3241.339 kb on - strand at 3241.339 kb on - strand at 3241.339 kb on - strand at 3241.379 kb on + strand at 3241.380 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmol 10 mM remove 3,239,018 + Psyr_2667 0.73 +1.6 3,239,021 + Psyr_2667 0.73 +1.1 3,239,108 + Psyr_2667 0.76 +0.2 3,239,245 - Psyr_2667 0.81 -0.4 3,239,245 - Psyr_2667 0.81 +0.5 3,239,382 + Psyr_2667 0.85 -1.1 3,239,483 - Psyr_2667 0.89 -0.4 3,239,525 + +0.5 3,239,525 + -0.9 3,239,525 + -2.9 3,239,526 - +0.6 3,239,526 - -0.3 3,239,526 - -1.5 3,239,531 - +0.5 3,239,569 - -2.7 3,239,688 + -0.1 3,239,689 - +0.1 3,239,689 - -0.6 3,239,715 + +2.6 3,239,715 + +0.2 3,239,715 + -2.1 3,239,716 - +1.2 3,239,716 - +0.1 3,239,718 - +1.3 3,239,718 - +1.1 3,239,718 - -0.9 3,239,802 + -1.3 3,239,848 + -0.2 3,239,848 + -1.8 3,239,940 + +0.1 3,240,026 + Psyr_2668 0.16 +1.2 3,240,026 + Psyr_2668 0.16 +5.2 3,240,026 + Psyr_2668 0.16 +1.5 3,240,026 + Psyr_2668 0.16 +1.0 3,240,030 + Psyr_2668 0.17 +1.0 3,240,030 + Psyr_2668 0.17 -0.7 3,240,030 + Psyr_2668 0.17 +1.5 3,240,030 + Psyr_2668 0.17 +1.1 3,240,030 + Psyr_2668 0.17 -0.0 3,240,030 + Psyr_2668 0.17 +0.5 3,240,030 + Psyr_2668 0.17 -1.5 3,240,030 + Psyr_2668 0.17 -0.2 3,240,030 + Psyr_2668 0.17 -0.2 3,240,030 + Psyr_2668 0.17 -0.1 3,240,426 - Psyr_2668 0.81 +1.6 3,240,484 - +0.1 3,240,536 + +2.1 3,240,536 + -1.1 3,240,536 + +1.3 3,240,536 + +3.2 3,240,536 + -0.8 3,240,536 + +1.4 3,240,536 + +0.7 3,240,537 - -3.1 3,240,537 - +0.9 3,240,540 + -1.4 3,240,541 - +0.1 3,241,329 + Psyr_2669 0.90 -0.1 3,241,338 + +1.5 3,241,338 + -1.7 3,241,339 - -0.2 3,241,339 - +0.0 3,241,339 - -0.9 3,241,379 + +0.1 3,241,380 - +5.0
Or see this region's nucleotide sequence