Experiment: KB with Harmol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0459 and Psyr_0460 are separated by 189 nucleotides Psyr_0460 and Psyr_0461 are separated by 354 nucleotides Psyr_0461 and Psyr_0462 are separated by 152 nucleotides
Psyr_0459: Psyr_0459 - 5,10-methylenetetrahydrofolate reductase, at 497,145 to 497,990
_0459
Psyr_0460: Psyr_0460 - adenosylhomocysteinase, at 498,180 to 499,589
_0460
Psyr_0461: Psyr_0461 - Thioesterase superfamily, at 499,944 to 500,339
_0461
Psyr_0462: Psyr_0462 - Hydroxypyruvate isomerase, at 500,492 to 501,277
_0462
Position (kb)
498
499
500 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 497.380 kb on + strand, within Psyr_0459 at 497.380 kb on + strand, within Psyr_0459 at 497.380 kb on + strand, within Psyr_0459 at 497.381 kb on - strand, within Psyr_0459 at 497.381 kb on - strand, within Psyr_0459 at 497.381 kb on - strand, within Psyr_0459 at 497.381 kb on - strand, within Psyr_0459 at 497.381 kb on - strand, within Psyr_0459 at 497.381 kb on - strand, within Psyr_0459 at 497.458 kb on + strand, within Psyr_0459 at 497.458 kb on + strand, within Psyr_0459 at 497.458 kb on + strand, within Psyr_0459 at 497.458 kb on + strand, within Psyr_0459 at 497.458 kb on + strand, within Psyr_0459 at 497.459 kb on - strand, within Psyr_0459 at 497.459 kb on - strand, within Psyr_0459 at 497.459 kb on - strand, within Psyr_0459 at 497.459 kb on - strand, within Psyr_0459 at 497.459 kb on - strand, within Psyr_0459 at 497.459 kb on - strand, within Psyr_0459 at 497.459 kb on - strand, within Psyr_0459 at 497.459 kb on - strand, within Psyr_0459 at 497.459 kb on - strand, within Psyr_0459 at 497.459 kb on - strand, within Psyr_0459 at 497.479 kb on + strand, within Psyr_0459 at 497.480 kb on - strand, within Psyr_0459 at 497.480 kb on - strand, within Psyr_0459 at 497.480 kb on - strand, within Psyr_0459 at 497.480 kb on - strand, within Psyr_0459 at 497.728 kb on + strand, within Psyr_0459 at 497.728 kb on + strand, within Psyr_0459 at 497.728 kb on + strand, within Psyr_0459 at 497.728 kb on + strand, within Psyr_0459 at 497.728 kb on + strand, within Psyr_0459 at 497.829 kb on - strand, within Psyr_0459 at 497.829 kb on - strand, within Psyr_0459 at 497.866 kb on + strand, within Psyr_0459 at 497.866 kb on + strand, within Psyr_0459 at 497.866 kb on + strand, within Psyr_0459 at 497.867 kb on - strand, within Psyr_0459 at 497.867 kb on - strand, within Psyr_0459 at 497.867 kb on - strand, within Psyr_0459 at 497.867 kb on - strand, within Psyr_0459 at 497.867 kb on - strand, within Psyr_0459 at 497.867 kb on - strand, within Psyr_0459 at 497.867 kb on - strand, within Psyr_0459 at 497.893 kb on + strand, within Psyr_0459 at 497.893 kb on + strand, within Psyr_0459 at 497.893 kb on + strand, within Psyr_0459 at 497.894 kb on - strand, within Psyr_0459 at 497.894 kb on - strand, within Psyr_0459 at 497.894 kb on - strand, within Psyr_0459 at 497.894 kb on - strand, within Psyr_0459 at 497.894 kb on - strand, within Psyr_0459 at 497.894 kb on - strand, within Psyr_0459 at 497.894 kb on - strand, within Psyr_0459 at 497.894 kb on - strand, within Psyr_0459 at 497.894 kb on - strand, within Psyr_0459 at 498.014 kb on - strand at 498.014 kb on - strand at 498.024 kb on + strand at 498.024 kb on + strand at 498.024 kb on + strand at 498.025 kb on - strand at 498.044 kb on + strand at 498.045 kb on - strand at 498.045 kb on - strand at 498.045 kb on - strand at 498.045 kb on - strand at 498.087 kb on + strand at 498.087 kb on + strand at 498.090 kb on + strand at 498.174 kb on + strand at 498.174 kb on + strand at 498.174 kb on + strand at 498.175 kb on - strand at 498.175 kb on - strand at 498.178 kb on + strand at 498.178 kb on + strand at 498.178 kb on + strand at 498.179 kb on - strand at 498.179 kb on - strand at 498.179 kb on - strand at 498.179 kb on - strand at 499.746 kb on + strand at 499.746 kb on + strand at 499.747 kb on - strand at 499.747 kb on - strand at 499.747 kb on - strand at 499.747 kb on - strand at 499.775 kb on - strand at 499.816 kb on + strand at 499.844 kb on + strand at 499.847 kb on + strand at 499.848 kb on - strand at 499.867 kb on + strand at 499.915 kb on - strand at 500.049 kb on + strand, within Psyr_0461 at 500.050 kb on - strand, within Psyr_0461 at 500.050 kb on - strand, within Psyr_0461 at 500.070 kb on - strand, within Psyr_0461 at 500.091 kb on + strand, within Psyr_0461 at 500.091 kb on + strand, within Psyr_0461 at 500.091 kb on + strand, within Psyr_0461 at 500.091 kb on + strand, within Psyr_0461 at 500.091 kb on + strand, within Psyr_0461 at 500.091 kb on + strand, within Psyr_0461 at 500.091 kb on + strand, within Psyr_0461 at 500.091 kb on + strand, within Psyr_0461 at 500.091 kb on + strand, within Psyr_0461 at 500.091 kb on + strand, within Psyr_0461 at 500.091 kb on + strand, within Psyr_0461 at 500.091 kb on + strand, within Psyr_0461 at 500.091 kb on + strand, within Psyr_0461 at 500.091 kb on + strand, within Psyr_0461 at 500.092 kb on - strand, within Psyr_0461 at 500.092 kb on - strand, within Psyr_0461 at 500.092 kb on - strand, within Psyr_0461 at 500.092 kb on - strand, within Psyr_0461 at 500.092 kb on - strand, within Psyr_0461 at 500.092 kb on - strand, within Psyr_0461 at 500.092 kb on - strand, within Psyr_0461 at 500.092 kb on - strand, within Psyr_0461 at 500.092 kb on - strand, within Psyr_0461 at 500.092 kb on - strand, within Psyr_0461 at 500.092 kb on - strand, within Psyr_0461 at 500.092 kb on - strand, within Psyr_0461 at 500.092 kb on - strand, within Psyr_0461 at 500.092 kb on - strand, within Psyr_0461 at 500.092 kb on - strand, within Psyr_0461 at 500.200 kb on + strand, within Psyr_0461 at 500.201 kb on - strand, within Psyr_0461 at 500.330 kb on - strand at 500.330 kb on - strand at 500.330 kb on - strand at 500.330 kb on - strand at 500.376 kb on + strand at 500.417 kb on + strand at 500.418 kb on - strand at 500.418 kb on - strand at 500.418 kb on - strand at 500.480 kb on + strand at 500.580 kb on + strand, within Psyr_0462
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmol 10 mM remove 497,380 + Psyr_0459 0.28 +1.8 497,380 + Psyr_0459 0.28 +2.2 497,380 + Psyr_0459 0.28 -0.1 497,381 - Psyr_0459 0.28 -2.7 497,381 - Psyr_0459 0.28 -0.4 497,381 - Psyr_0459 0.28 -1.2 497,381 - Psyr_0459 0.28 -0.4 497,381 - Psyr_0459 0.28 -2.4 497,381 - Psyr_0459 0.28 +1.3 497,458 + Psyr_0459 0.37 +0.8 497,458 + Psyr_0459 0.37 -0.8 497,458 + Psyr_0459 0.37 -0.8 497,458 + Psyr_0459 0.37 -1.4 497,458 + Psyr_0459 0.37 -0.8 497,459 - Psyr_0459 0.37 -0.6 497,459 - Psyr_0459 0.37 -0.1 497,459 - Psyr_0459 0.37 -0.6 497,459 - Psyr_0459 0.37 -0.8 497,459 - Psyr_0459 0.37 -0.3 497,459 - Psyr_0459 0.37 -2.0 497,459 - Psyr_0459 0.37 +1.2 497,459 - Psyr_0459 0.37 +1.3 497,459 - Psyr_0459 0.37 -0.8 497,459 - Psyr_0459 0.37 -1.8 497,479 + Psyr_0459 0.39 -1.5 497,480 - Psyr_0459 0.40 -0.8 497,480 - Psyr_0459 0.40 +0.2 497,480 - Psyr_0459 0.40 +1.3 497,480 - Psyr_0459 0.40 +3.0 497,728 + Psyr_0459 0.69 -1.5 497,728 + Psyr_0459 0.69 +0.7 497,728 + Psyr_0459 0.69 +1.6 497,728 + Psyr_0459 0.69 +0.9 497,728 + Psyr_0459 0.69 +1.2 497,829 - Psyr_0459 0.81 +0.9 497,829 - Psyr_0459 0.81 +0.5 497,866 + Psyr_0459 0.85 -0.5 497,866 + Psyr_0459 0.85 +1.2 497,866 + Psyr_0459 0.85 -0.4 497,867 - Psyr_0459 0.85 +0.6 497,867 - Psyr_0459 0.85 +0.1 497,867 - Psyr_0459 0.85 -0.7 497,867 - Psyr_0459 0.85 +2.6 497,867 - Psyr_0459 0.85 +0.4 497,867 - Psyr_0459 0.85 +0.5 497,867 - Psyr_0459 0.85 +0.3 497,893 + Psyr_0459 0.88 -0.6 497,893 + Psyr_0459 0.88 +0.0 497,893 + Psyr_0459 0.88 +0.2 497,894 - Psyr_0459 0.89 +2.0 497,894 - Psyr_0459 0.89 -1.8 497,894 - Psyr_0459 0.89 +0.8 497,894 - Psyr_0459 0.89 -0.2 497,894 - Psyr_0459 0.89 +0.5 497,894 - Psyr_0459 0.89 +0.3 497,894 - Psyr_0459 0.89 +0.1 497,894 - Psyr_0459 0.89 -0.4 497,894 - Psyr_0459 0.89 +1.2 498,014 - -3.7 498,014 - +2.0 498,024 + +0.2 498,024 + +0.8 498,024 + -2.5 498,025 - -0.2 498,044 + +1.4 498,045 - -0.1 498,045 - -0.8 498,045 - -0.1 498,045 - -0.6 498,087 + -0.4 498,087 + +5.1 498,090 + +0.8 498,174 + +0.9 498,174 + +0.4 498,174 + +0.3 498,175 - +0.8 498,175 - +0.2 498,178 + +2.2 498,178 + +3.4 498,178 + +1.3 498,179 - +0.4 498,179 - +0.3 498,179 - -0.8 498,179 - -0.3 499,746 + +1.2 499,746 + -1.6 499,747 - +1.2 499,747 - -1.0 499,747 - +0.8 499,747 - -0.6 499,775 - -0.4 499,816 + +0.2 499,844 + +0.2 499,847 + +0.2 499,848 - +0.1 499,867 + -0.8 499,915 - -0.7 500,049 + Psyr_0461 0.27 +2.5 500,050 - Psyr_0461 0.27 +0.2 500,050 - Psyr_0461 0.27 +0.6 500,070 - Psyr_0461 0.32 -0.3 500,091 + Psyr_0461 0.37 +0.9 500,091 + Psyr_0461 0.37 +0.2 500,091 + Psyr_0461 0.37 -0.4 500,091 + Psyr_0461 0.37 -1.0 500,091 + Psyr_0461 0.37 +0.8 500,091 + Psyr_0461 0.37 +1.2 500,091 + Psyr_0461 0.37 +0.8 500,091 + Psyr_0461 0.37 +0.7 500,091 + Psyr_0461 0.37 +2.8 500,091 + Psyr_0461 0.37 +0.1 500,091 + Psyr_0461 0.37 +1.8 500,091 + Psyr_0461 0.37 -0.2 500,091 + Psyr_0461 0.37 -2.6 500,091 + Psyr_0461 0.37 +0.2 500,092 - Psyr_0461 0.37 +0.2 500,092 - Psyr_0461 0.37 -0.4 500,092 - Psyr_0461 0.37 +0.4 500,092 - Psyr_0461 0.37 +1.0 500,092 - Psyr_0461 0.37 -0.2 500,092 - Psyr_0461 0.37 -0.4 500,092 - Psyr_0461 0.37 +0.2 500,092 - Psyr_0461 0.37 -0.4 500,092 - Psyr_0461 0.37 -1.4 500,092 - Psyr_0461 0.37 +0.9 500,092 - Psyr_0461 0.37 +0.1 500,092 - Psyr_0461 0.37 -1.0 500,092 - Psyr_0461 0.37 +2.2 500,092 - Psyr_0461 0.37 +1.1 500,092 - Psyr_0461 0.37 -1.3 500,200 + Psyr_0461 0.65 +1.0 500,201 - Psyr_0461 0.65 +0.7 500,330 - +0.0 500,330 - -1.5 500,330 - -3.2 500,330 - -0.7 500,376 + +0.3 500,417 + -1.3 500,418 - +0.9 500,418 - +0.1 500,418 - -0.7 500,480 + -2.1 500,580 + Psyr_0462 0.11 +2.2
Or see this region's nucleotide sequence