Experiment: KB with Harmol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0275 and Psyr_0276 are separated by 67 nucleotides Psyr_0276 and Psyr_0277 overlap by 1 nucleotides Psyr_0277 and Psyr_0278 overlap by 8 nucleotides
Psyr_0275: Psyr_0275 - Periplasmic solute binding protein, at 293,509 to 294,450
_0275
Psyr_0276: Psyr_0276 - zinc uptake regulation protein, putative, at 294,518 to 295,000
_0276
Psyr_0277: Psyr_0277 - ABC transporter, at 295,000 to 295,788
_0277
Psyr_0278: Psyr_0278 - ABC-3, at 295,781 to 296,569
_0278
Position (kb) 294
295
296 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 293.518 kb on - strand at 293.518 kb on - strand at 293.591 kb on + strand at 293.793 kb on - strand, within Psyr_0275 at 293.793 kb on - strand, within Psyr_0275 at 293.793 kb on - strand, within Psyr_0275 at 293.807 kb on + strand, within Psyr_0275 at 293.834 kb on + strand, within Psyr_0275 at 293.834 kb on + strand, within Psyr_0275 at 293.835 kb on - strand, within Psyr_0275 at 293.835 kb on - strand, within Psyr_0275 at 293.835 kb on - strand, within Psyr_0275 at 293.835 kb on - strand, within Psyr_0275 at 294.219 kb on - strand, within Psyr_0275 at 294.238 kb on + strand, within Psyr_0275 at 294.247 kb on + strand, within Psyr_0275 at 294.269 kb on + strand, within Psyr_0275 at 294.270 kb on - strand, within Psyr_0275 at 294.273 kb on + strand, within Psyr_0275 at 294.273 kb on + strand, within Psyr_0275 at 294.273 kb on + strand, within Psyr_0275 at 294.313 kb on - strand, within Psyr_0275 at 294.328 kb on + strand, within Psyr_0275 at 294.436 kb on + strand at 294.463 kb on + strand at 294.463 kb on + strand at 294.463 kb on + strand at 294.485 kb on + strand at 294.485 kb on + strand at 294.485 kb on + strand at 294.485 kb on + strand at 294.485 kb on + strand at 294.485 kb on + strand at 294.485 kb on + strand at 294.485 kb on + strand at 294.486 kb on - strand at 294.486 kb on - strand at 294.488 kb on + strand at 294.526 kb on + strand at 294.526 kb on + strand at 294.597 kb on + strand, within Psyr_0276 at 294.598 kb on - strand, within Psyr_0276 at 294.598 kb on - strand, within Psyr_0276 at 294.683 kb on + strand, within Psyr_0276 at 294.683 kb on + strand, within Psyr_0276 at 294.684 kb on - strand, within Psyr_0276 at 294.684 kb on - strand, within Psyr_0276 at 294.684 kb on - strand, within Psyr_0276 at 295.016 kb on + strand at 295.016 kb on + strand at 295.016 kb on + strand at 295.016 kb on + strand at 295.016 kb on + strand at 295.016 kb on + strand at 295.016 kb on + strand at 295.017 kb on - strand at 295.017 kb on - strand at 295.214 kb on - strand, within Psyr_0277 at 295.460 kb on + strand, within Psyr_0277 at 295.460 kb on + strand, within Psyr_0277 at 295.460 kb on + strand, within Psyr_0277 at 295.480 kb on + strand, within Psyr_0277 at 295.681 kb on + strand, within Psyr_0277 at 295.681 kb on + strand, within Psyr_0277 at 295.682 kb on - strand, within Psyr_0277 at 295.682 kb on - strand, within Psyr_0277 at 295.682 kb on - strand, within Psyr_0277 at 295.682 kb on - strand, within Psyr_0277 at 295.776 kb on + strand at 295.776 kb on + strand at 295.777 kb on - strand at 295.777 kb on - strand at 295.777 kb on - strand at 295.777 kb on - strand at 295.777 kb on - strand at 295.799 kb on + strand at 295.799 kb on + strand at 295.799 kb on + strand at 295.799 kb on + strand at 295.799 kb on + strand at 295.799 kb on + strand at 295.799 kb on + strand at 295.799 kb on + strand at 295.800 kb on - strand at 295.800 kb on - strand at 295.800 kb on - strand at 295.800 kb on - strand at 295.800 kb on - strand at 295.800 kb on - strand at 295.877 kb on + strand, within Psyr_0278
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmol 10 mM remove 293,518 - +1.6 293,518 - -0.7 293,591 + +0.1 293,793 - Psyr_0275 0.30 +2.1 293,793 - Psyr_0275 0.30 +4.0 293,793 - Psyr_0275 0.30 +0.6 293,807 + Psyr_0275 0.32 -1.4 293,834 + Psyr_0275 0.35 -4.1 293,834 + Psyr_0275 0.35 -1.0 293,835 - Psyr_0275 0.35 +0.4 293,835 - Psyr_0275 0.35 +0.3 293,835 - Psyr_0275 0.35 -0.2 293,835 - Psyr_0275 0.35 -0.8 294,219 - Psyr_0275 0.75 -1.9 294,238 + Psyr_0275 0.77 +1.2 294,247 + Psyr_0275 0.78 -0.6 294,269 + Psyr_0275 0.81 -0.3 294,270 - Psyr_0275 0.81 +0.3 294,273 + Psyr_0275 0.81 -0.1 294,273 + Psyr_0275 0.81 +0.8 294,273 + Psyr_0275 0.81 +0.8 294,313 - Psyr_0275 0.85 -1.3 294,328 + Psyr_0275 0.87 +0.6 294,436 + -2.3 294,463 + -0.7 294,463 + +3.7 294,463 + +1.4 294,485 + -1.4 294,485 + +0.6 294,485 + +0.9 294,485 + -2.3 294,485 + -0.8 294,485 + -0.6 294,485 + +0.5 294,485 + +0.8 294,486 - +0.3 294,486 - -0.3 294,488 + +0.1 294,526 + +0.0 294,526 + -0.7 294,597 + Psyr_0276 0.16 -0.7 294,598 - Psyr_0276 0.17 +0.0 294,598 - Psyr_0276 0.17 -0.2 294,683 + Psyr_0276 0.34 +0.1 294,683 + Psyr_0276 0.34 -1.7 294,684 - Psyr_0276 0.34 +0.1 294,684 - Psyr_0276 0.34 -1.0 294,684 - Psyr_0276 0.34 +0.9 295,016 + -0.8 295,016 + +0.9 295,016 + -1.4 295,016 + -0.1 295,016 + -1.3 295,016 + +1.8 295,016 + +1.0 295,017 - +0.2 295,017 - -0.1 295,214 - Psyr_0277 0.27 +0.1 295,460 + Psyr_0277 0.58 -1.3 295,460 + Psyr_0277 0.58 +0.2 295,460 + Psyr_0277 0.58 +1.9 295,480 + Psyr_0277 0.61 -0.0 295,681 + Psyr_0277 0.86 -3.0 295,681 + Psyr_0277 0.86 -0.3 295,682 - Psyr_0277 0.86 +1.9 295,682 - Psyr_0277 0.86 +0.2 295,682 - Psyr_0277 0.86 -2.6 295,682 - Psyr_0277 0.86 +1.8 295,776 + -0.0 295,776 + -1.4 295,777 - -2.3 295,777 - +0.9 295,777 - +1.5 295,777 - -1.7 295,777 - -0.1 295,799 + -0.4 295,799 + -0.1 295,799 + +0.4 295,799 + +5.3 295,799 + -0.2 295,799 + +1.1 295,799 + -2.1 295,799 + +2.5 295,800 - -1.2 295,800 - -0.4 295,800 - -0.5 295,800 - +0.5 295,800 - +1.0 295,800 - -0.8 295,877 + Psyr_0278 0.12 +1.0
Or see this region's nucleotide sequence