Experiment: KB with Harmol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0210 and Psyr_0211 are separated by 147 nucleotides Psyr_0211 and Psyr_0212 are separated by 13 nucleotides Psyr_0212 and Psyr_0213 are separated by 312 nucleotides
Psyr_0210: Psyr_0210 - DNA-directed RNA polymerase subunit omega, at 225,170 to 225,433
_0210
Psyr_0211: Psyr_0211 - guanylate kinase, at 225,581 to 226,225
_0211
Psyr_0212: Psyr_0212 - Conserved hypothetical protein 255, at 226,239 to 227,102
_0212
Psyr_0213: Psyr_0213 - RNAse PH, at 227,415 to 228,137
_0213
Position (kb) 226
227
228 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 225.253 kb on - strand, within Psyr_0210 at 225.405 kb on - strand, within Psyr_0210 at 225.438 kb on - strand at 225.497 kb on - strand at 225.505 kb on - strand at 225.505 kb on - strand at 225.613 kb on - strand at 225.613 kb on - strand at 226.235 kb on - strand at 226.241 kb on - strand at 226.248 kb on - strand at 226.341 kb on - strand, within Psyr_0212 at 226.341 kb on - strand, within Psyr_0212 at 226.341 kb on - strand, within Psyr_0212 at 226.341 kb on - strand, within Psyr_0212 at 226.341 kb on - strand, within Psyr_0212 at 227.071 kb on - strand at 227.110 kb on - strand at 227.110 kb on - strand at 227.196 kb on - strand at 227.212 kb on - strand at 227.344 kb on + strand at 227.344 kb on + strand at 227.345 kb on - strand at 227.345 kb on - strand at 227.373 kb on + strand at 227.374 kb on - strand at 227.374 kb on - strand at 227.374 kb on - strand at 227.374 kb on - strand at 227.374 kb on - strand at 227.375 kb on + strand at 227.376 kb on - strand at 227.376 kb on - strand at 227.402 kb on - strand at 227.408 kb on - strand at 227.475 kb on + strand at 227.475 kb on + strand at 227.501 kb on - strand, within Psyr_0213 at 227.520 kb on - strand, within Psyr_0213 at 227.637 kb on - strand, within Psyr_0213 at 227.675 kb on + strand, within Psyr_0213 at 227.675 kb on + strand, within Psyr_0213 at 227.675 kb on + strand, within Psyr_0213 at 227.676 kb on - strand, within Psyr_0213 at 227.754 kb on + strand, within Psyr_0213 at 227.755 kb on - strand, within Psyr_0213 at 227.755 kb on - strand, within Psyr_0213 at 227.755 kb on - strand, within Psyr_0213 at 227.795 kb on + strand, within Psyr_0213 at 227.796 kb on - strand, within Psyr_0213 at 227.804 kb on + strand, within Psyr_0213 at 227.805 kb on - strand, within Psyr_0213 at 227.805 kb on - strand, within Psyr_0213 at 227.805 kb on - strand, within Psyr_0213 at 227.805 kb on - strand, within Psyr_0213 at 227.919 kb on + strand, within Psyr_0213 at 227.919 kb on + strand, within Psyr_0213 at 227.919 kb on + strand, within Psyr_0213 at 227.919 kb on + strand, within Psyr_0213 at 227.920 kb on - strand, within Psyr_0213 at 227.920 kb on - strand, within Psyr_0213 at 227.920 kb on - strand, within Psyr_0213 at 227.950 kb on - strand, within Psyr_0213
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmol 10 mM remove 225,253 - Psyr_0210 0.31 -0.6 225,405 - Psyr_0210 0.89 -2.2 225,438 - -0.2 225,497 - -0.2 225,505 - -1.3 225,505 - -2.2 225,613 - -2.5 225,613 - -0.5 226,235 - -2.2 226,241 - +0.7 226,248 - -1.8 226,341 - Psyr_0212 0.12 -2.0 226,341 - Psyr_0212 0.12 -1.9 226,341 - Psyr_0212 0.12 -1.8 226,341 - Psyr_0212 0.12 -0.0 226,341 - Psyr_0212 0.12 +1.2 227,071 - +0.5 227,110 - +0.5 227,110 - -0.4 227,196 - +0.2 227,212 - +0.2 227,344 + +2.0 227,344 + -0.8 227,345 - -3.2 227,345 - -0.4 227,373 + +0.4 227,374 - +0.9 227,374 - -0.4 227,374 - +2.2 227,374 - -0.0 227,374 - +0.2 227,375 + +1.0 227,376 - -1.7 227,376 - +0.0 227,402 - -2.4 227,408 - -0.7 227,475 + +1.8 227,475 + +0.9 227,501 - Psyr_0213 0.12 -1.6 227,520 - Psyr_0213 0.15 -1.6 227,637 - Psyr_0213 0.31 +0.8 227,675 + Psyr_0213 0.36 -0.2 227,675 + Psyr_0213 0.36 -0.4 227,675 + Psyr_0213 0.36 -0.4 227,676 - Psyr_0213 0.36 +0.2 227,754 + Psyr_0213 0.47 +1.2 227,755 - Psyr_0213 0.47 +4.0 227,755 - Psyr_0213 0.47 -1.6 227,755 - Psyr_0213 0.47 -0.5 227,795 + Psyr_0213 0.53 +1.4 227,796 - Psyr_0213 0.53 +1.5 227,804 + Psyr_0213 0.54 -1.2 227,805 - Psyr_0213 0.54 -0.4 227,805 - Psyr_0213 0.54 +0.8 227,805 - Psyr_0213 0.54 -0.1 227,805 - Psyr_0213 0.54 -0.4 227,919 + Psyr_0213 0.70 -0.1 227,919 + Psyr_0213 0.70 -0.3 227,919 + Psyr_0213 0.70 -0.6 227,919 + Psyr_0213 0.70 -1.5 227,920 - Psyr_0213 0.70 +3.0 227,920 - Psyr_0213 0.70 -2.9 227,920 - Psyr_0213 0.70 +1.4 227,950 - Psyr_0213 0.74 -2.2
Or see this region's nucleotide sequence