Experiment: KB with Auraptene 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2019 and Psyr_2020 are separated by 10 nucleotides Psyr_2020 and Psyr_2021 are separated by 221 nucleotides Psyr_2021 and Psyr_2022 are separated by 284 nucleotides
Psyr_2019: Psyr_2019 - 3-oxoacyl-[acyl-carrier-protein] synthase I, at 2,343,838 to 2,345,061
_2019
Psyr_2020: Psyr_2020 - 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase, at 2,345,072 to 2,345,587
_2020
Psyr_2021: Psyr_2021 - Response regulator receiver:ATP-binding region, ATPase-like:Histidine kinase, HAMP region:Histidine kinase A, N-terminal, at 2,345,809 to 2,347,746
_2021
Psyr_2022: Psyr_2022 - Glycerol-3-phosphate dehydrogenase (NAD(P)+), at 2,348,031 to 2,349,056
_2022
Position (kb)
2345
2346
2347
2348 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2345.692 kb on - strand at 2345.870 kb on - strand at 2346.220 kb on + strand, within Psyr_2021 at 2346.321 kb on - strand, within Psyr_2021 at 2346.734 kb on - strand, within Psyr_2021 at 2347.243 kb on + strand, within Psyr_2021 at 2347.244 kb on - strand, within Psyr_2021 at 2347.244 kb on - strand, within Psyr_2021 at 2347.305 kb on - strand, within Psyr_2021 at 2347.450 kb on + strand, within Psyr_2021 at 2347.450 kb on + strand, within Psyr_2021 at 2347.451 kb on - strand, within Psyr_2021 at 2347.451 kb on - strand, within Psyr_2021 at 2347.451 kb on - strand, within Psyr_2021 at 2347.454 kb on + strand, within Psyr_2021 at 2347.454 kb on + strand, within Psyr_2021 at 2347.455 kb on - strand, within Psyr_2021 at 2347.455 kb on - strand, within Psyr_2021 at 2347.455 kb on - strand, within Psyr_2021 at 2347.455 kb on - strand, within Psyr_2021 at 2347.455 kb on - strand, within Psyr_2021 at 2347.455 kb on - strand, within Psyr_2021 at 2347.455 kb on - strand, within Psyr_2021 at 2347.641 kb on + strand at 2347.642 kb on - strand at 2347.646 kb on - strand at 2347.646 kb on - strand at 2347.646 kb on - strand at 2347.722 kb on + strand at 2347.722 kb on + strand at 2347.722 kb on + strand at 2347.722 kb on + strand at 2347.723 kb on - strand at 2347.723 kb on - strand at 2347.933 kb on - strand at 2347.933 kb on - strand at 2347.933 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Auraptene 10 mM remove 2,345,692 - +0.2 2,345,870 - +0.5 2,346,220 + Psyr_2021 0.21 -1.2 2,346,321 - Psyr_2021 0.26 -0.7 2,346,734 - Psyr_2021 0.48 -0.9 2,347,243 + Psyr_2021 0.74 -1.3 2,347,244 - Psyr_2021 0.74 -1.6 2,347,244 - Psyr_2021 0.74 -0.1 2,347,305 - Psyr_2021 0.77 -0.1 2,347,450 + Psyr_2021 0.85 -1.3 2,347,450 + Psyr_2021 0.85 -0.3 2,347,451 - Psyr_2021 0.85 +1.9 2,347,451 - Psyr_2021 0.85 +4.2 2,347,451 - Psyr_2021 0.85 +1.7 2,347,454 + Psyr_2021 0.85 +0.2 2,347,454 + Psyr_2021 0.85 +0.2 2,347,455 - Psyr_2021 0.85 +0.1 2,347,455 - Psyr_2021 0.85 -1.4 2,347,455 - Psyr_2021 0.85 -1.0 2,347,455 - Psyr_2021 0.85 +0.3 2,347,455 - Psyr_2021 0.85 -1.8 2,347,455 - Psyr_2021 0.85 -0.8 2,347,455 - Psyr_2021 0.85 -0.7 2,347,641 + -1.6 2,347,642 - +0.5 2,347,646 - -0.6 2,347,646 - -1.1 2,347,646 - +0.3 2,347,722 + -0.8 2,347,722 + -0.9 2,347,722 + +2.0 2,347,722 + +1.9 2,347,723 - +0.7 2,347,723 - -1.6 2,347,933 - -0.8 2,347,933 - -1.3 2,347,933 - -0.1
Or see this region's nucleotide sequence