Strain Fitness in Pseudomonas syringae pv. syringae B728a ΔmexB around Psyr_2021

Experiment: KB with Auraptene 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_2019 and Psyr_2020 are separated by 10 nucleotidesPsyr_2020 and Psyr_2021 are separated by 221 nucleotidesPsyr_2021 and Psyr_2022 are separated by 284 nucleotides Psyr_2019: Psyr_2019 - 3-oxoacyl-[acyl-carrier-protein] synthase I, at 2,343,838 to 2,345,061 _2019 Psyr_2020: Psyr_2020 - 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase, at 2,345,072 to 2,345,587 _2020 Psyr_2021: Psyr_2021 - Response regulator receiver:ATP-binding region, ATPase-like:Histidine kinase, HAMP region:Histidine kinase A, N-terminal, at 2,345,809 to 2,347,746 _2021 Psyr_2022: Psyr_2022 - Glycerol-3-phosphate dehydrogenase (NAD(P)+), at 2,348,031 to 2,349,056 _2022 Position (kb) 2345 2346 2347 2348Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 2345.692 kb on - strandat 2345.870 kb on - strandat 2346.220 kb on + strand, within Psyr_2021at 2346.321 kb on - strand, within Psyr_2021at 2346.734 kb on - strand, within Psyr_2021at 2347.243 kb on + strand, within Psyr_2021at 2347.244 kb on - strand, within Psyr_2021at 2347.244 kb on - strand, within Psyr_2021at 2347.305 kb on - strand, within Psyr_2021at 2347.450 kb on + strand, within Psyr_2021at 2347.450 kb on + strand, within Psyr_2021at 2347.451 kb on - strand, within Psyr_2021at 2347.451 kb on - strand, within Psyr_2021at 2347.451 kb on - strand, within Psyr_2021at 2347.454 kb on + strand, within Psyr_2021at 2347.454 kb on + strand, within Psyr_2021at 2347.455 kb on - strand, within Psyr_2021at 2347.455 kb on - strand, within Psyr_2021at 2347.455 kb on - strand, within Psyr_2021at 2347.455 kb on - strand, within Psyr_2021at 2347.455 kb on - strand, within Psyr_2021at 2347.455 kb on - strand, within Psyr_2021at 2347.455 kb on - strand, within Psyr_2021at 2347.641 kb on + strandat 2347.642 kb on - strandat 2347.646 kb on - strandat 2347.646 kb on - strandat 2347.646 kb on - strandat 2347.722 kb on + strandat 2347.722 kb on + strandat 2347.722 kb on + strandat 2347.722 kb on + strandat 2347.723 kb on - strandat 2347.723 kb on - strandat 2347.933 kb on - strandat 2347.933 kb on - strandat 2347.933 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Auraptene 10 mM
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2,345,692 - +0.2
2,345,870 - +0.5
2,346,220 + Psyr_2021 0.21 -1.2
2,346,321 - Psyr_2021 0.26 -0.7
2,346,734 - Psyr_2021 0.48 -0.9
2,347,243 + Psyr_2021 0.74 -1.3
2,347,244 - Psyr_2021 0.74 -1.6
2,347,244 - Psyr_2021 0.74 -0.1
2,347,305 - Psyr_2021 0.77 -0.1
2,347,450 + Psyr_2021 0.85 -1.3
2,347,450 + Psyr_2021 0.85 -0.3
2,347,451 - Psyr_2021 0.85 +1.9
2,347,451 - Psyr_2021 0.85 +4.2
2,347,451 - Psyr_2021 0.85 +1.7
2,347,454 + Psyr_2021 0.85 +0.2
2,347,454 + Psyr_2021 0.85 +0.2
2,347,455 - Psyr_2021 0.85 +0.1
2,347,455 - Psyr_2021 0.85 -1.4
2,347,455 - Psyr_2021 0.85 -1.0
2,347,455 - Psyr_2021 0.85 +0.3
2,347,455 - Psyr_2021 0.85 -1.8
2,347,455 - Psyr_2021 0.85 -0.8
2,347,455 - Psyr_2021 0.85 -0.7
2,347,641 + -1.6
2,347,642 - +0.5
2,347,646 - -0.6
2,347,646 - -1.1
2,347,646 - +0.3
2,347,722 + -0.8
2,347,722 + -0.9
2,347,722 + +2.0
2,347,722 + +1.9
2,347,723 - +0.7
2,347,723 - -1.6
2,347,933 - -0.8
2,347,933 - -1.3
2,347,933 - -0.1

Or see this region's nucleotide sequence