Experiment: KB with Auraptene 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1133 and Psyr_1134 overlap by 4 nucleotides Psyr_1134 and Psyr_1135 are separated by 1 nucleotides
Psyr_1133: Psyr_1133 - Fimbrial biogenesis outer membrane usher protein, at 1,283,830 to 1,286,364
_1133
Psyr_1134: Psyr_1134 - pili assembly chaperone, at 1,286,361 to 1,287,146
_1134
Psyr_1135: Psyr_1135 - conserved hypothetical protein, at 1,287,148 to 1,288,146
_1135
Position (kb)
1286
1287
1288 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 1285.365 kb on - strand, within Psyr_1133 at 1285.365 kb on - strand, within Psyr_1133 at 1285.447 kb on + strand, within Psyr_1133 at 1285.448 kb on - strand, within Psyr_1133 at 1285.448 kb on - strand, within Psyr_1133 at 1285.448 kb on - strand, within Psyr_1133 at 1285.456 kb on + strand, within Psyr_1133 at 1285.456 kb on + strand, within Psyr_1133 at 1285.456 kb on + strand, within Psyr_1133 at 1285.456 kb on + strand, within Psyr_1133 at 1285.457 kb on - strand, within Psyr_1133 at 1285.457 kb on - strand, within Psyr_1133 at 1285.457 kb on - strand, within Psyr_1133 at 1285.457 kb on - strand, within Psyr_1133 at 1285.457 kb on - strand, within Psyr_1133 at 1285.457 kb on - strand, within Psyr_1133 at 1285.457 kb on - strand, within Psyr_1133 at 1285.458 kb on + strand, within Psyr_1133 at 1285.483 kb on + strand, within Psyr_1133 at 1285.483 kb on + strand, within Psyr_1133 at 1285.636 kb on + strand, within Psyr_1133 at 1285.691 kb on - strand, within Psyr_1133 at 1285.691 kb on - strand, within Psyr_1133 at 1285.698 kb on - strand, within Psyr_1133 at 1285.786 kb on - strand, within Psyr_1133 at 1285.802 kb on - strand, within Psyr_1133 at 1285.802 kb on - strand, within Psyr_1133 at 1285.802 kb on - strand, within Psyr_1133 at 1285.938 kb on + strand, within Psyr_1133 at 1286.056 kb on - strand, within Psyr_1133 at 1286.380 kb on + strand at 1286.531 kb on - strand, within Psyr_1134 at 1286.709 kb on + strand, within Psyr_1134 at 1286.709 kb on + strand, within Psyr_1134 at 1286.709 kb on + strand, within Psyr_1134 at 1286.709 kb on + strand, within Psyr_1134 at 1286.709 kb on + strand, within Psyr_1134 at 1286.709 kb on + strand, within Psyr_1134 at 1286.710 kb on - strand, within Psyr_1134 at 1286.710 kb on - strand, within Psyr_1134 at 1286.710 kb on - strand, within Psyr_1134 at 1286.710 kb on - strand, within Psyr_1134 at 1286.710 kb on - strand, within Psyr_1134 at 1286.710 kb on - strand, within Psyr_1134 at 1286.710 kb on - strand, within Psyr_1134 at 1286.710 kb on - strand, within Psyr_1134 at 1286.710 kb on - strand, within Psyr_1134 at 1286.710 kb on - strand, within Psyr_1134 at 1286.902 kb on - strand, within Psyr_1134 at 1287.148 kb on - strand at 1287.324 kb on - strand, within Psyr_1135 at 1287.399 kb on + strand, within Psyr_1135 at 1287.399 kb on + strand, within Psyr_1135 at 1287.400 kb on - strand, within Psyr_1135 at 1287.400 kb on - strand, within Psyr_1135 at 1287.403 kb on + strand, within Psyr_1135 at 1287.403 kb on + strand, within Psyr_1135 at 1287.404 kb on - strand, within Psyr_1135 at 1287.508 kb on + strand, within Psyr_1135 at 1287.706 kb on + strand, within Psyr_1135 at 1287.706 kb on + strand, within Psyr_1135 at 1287.707 kb on - strand, within Psyr_1135 at 1287.707 kb on - strand, within Psyr_1135 at 1287.707 kb on - strand, within Psyr_1135 at 1287.707 kb on - strand, within Psyr_1135 at 1287.963 kb on - strand, within Psyr_1135 at 1287.969 kb on + strand, within Psyr_1135 at 1287.995 kb on + strand, within Psyr_1135 at 1288.000 kb on + strand, within Psyr_1135 at 1288.000 kb on + strand, within Psyr_1135 at 1288.001 kb on - strand, within Psyr_1135 at 1288.138 kb on + strand at 1288.139 kb on - strand at 1288.139 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Auraptene 10 mM remove 1,285,365 - Psyr_1133 0.61 -0.7 1,285,365 - Psyr_1133 0.61 -0.1 1,285,447 + Psyr_1133 0.64 +0.9 1,285,448 - Psyr_1133 0.64 -0.1 1,285,448 - Psyr_1133 0.64 -0.7 1,285,448 - Psyr_1133 0.64 +1.3 1,285,456 + Psyr_1133 0.64 +1.3 1,285,456 + Psyr_1133 0.64 -0.4 1,285,456 + Psyr_1133 0.64 -0.1 1,285,456 + Psyr_1133 0.64 -0.7 1,285,457 - Psyr_1133 0.64 +0.0 1,285,457 - Psyr_1133 0.64 -0.2 1,285,457 - Psyr_1133 0.64 +1.5 1,285,457 - Psyr_1133 0.64 -1.8 1,285,457 - Psyr_1133 0.64 +1.5 1,285,457 - Psyr_1133 0.64 +4.2 1,285,457 - Psyr_1133 0.64 +0.2 1,285,458 + Psyr_1133 0.64 +3.4 1,285,483 + Psyr_1133 0.65 +0.0 1,285,483 + Psyr_1133 0.65 +4.5 1,285,636 + Psyr_1133 0.71 +2.0 1,285,691 - Psyr_1133 0.73 +0.3 1,285,691 - Psyr_1133 0.73 +1.2 1,285,698 - Psyr_1133 0.74 +0.9 1,285,786 - Psyr_1133 0.77 +0.6 1,285,802 - Psyr_1133 0.78 +1.0 1,285,802 - Psyr_1133 0.78 -0.5 1,285,802 - Psyr_1133 0.78 +4.9 1,285,938 + Psyr_1133 0.83 -0.1 1,286,056 - Psyr_1133 0.88 +0.2 1,286,380 + -3.0 1,286,531 - Psyr_1134 0.22 -0.0 1,286,709 + Psyr_1134 0.44 +0.2 1,286,709 + Psyr_1134 0.44 -2.3 1,286,709 + Psyr_1134 0.44 -0.3 1,286,709 + Psyr_1134 0.44 +0.7 1,286,709 + Psyr_1134 0.44 +0.5 1,286,709 + Psyr_1134 0.44 -0.1 1,286,710 - Psyr_1134 0.44 -0.1 1,286,710 - Psyr_1134 0.44 +0.5 1,286,710 - Psyr_1134 0.44 -1.8 1,286,710 - Psyr_1134 0.44 -1.5 1,286,710 - Psyr_1134 0.44 +1.0 1,286,710 - Psyr_1134 0.44 +0.0 1,286,710 - Psyr_1134 0.44 +0.8 1,286,710 - Psyr_1134 0.44 -1.1 1,286,710 - Psyr_1134 0.44 +2.5 1,286,710 - Psyr_1134 0.44 -0.5 1,286,902 - Psyr_1134 0.69 +0.9 1,287,148 - +0.7 1,287,324 - Psyr_1135 0.18 +0.5 1,287,399 + Psyr_1135 0.25 -2.7 1,287,399 + Psyr_1135 0.25 -1.3 1,287,400 - Psyr_1135 0.25 -0.1 1,287,400 - Psyr_1135 0.25 +0.3 1,287,403 + Psyr_1135 0.26 -1.2 1,287,403 + Psyr_1135 0.26 +0.0 1,287,404 - Psyr_1135 0.26 -0.2 1,287,508 + Psyr_1135 0.36 +0.2 1,287,706 + Psyr_1135 0.56 +1.3 1,287,706 + Psyr_1135 0.56 +1.0 1,287,707 - Psyr_1135 0.56 +0.0 1,287,707 - Psyr_1135 0.56 +1.3 1,287,707 - Psyr_1135 0.56 +0.0 1,287,707 - Psyr_1135 0.56 -0.5 1,287,963 - Psyr_1135 0.82 +1.0 1,287,969 + Psyr_1135 0.82 -0.9 1,287,995 + Psyr_1135 0.85 +3.0 1,288,000 + Psyr_1135 0.85 -0.6 1,288,000 + Psyr_1135 0.85 +0.3 1,288,001 - Psyr_1135 0.85 +0.5 1,288,138 + -0.5 1,288,139 - -1.4 1,288,139 - -0.5
Or see this region's nucleotide sequence