Strain Fitness in Pseudomonas syringae pv. syringae B728a ΔmexB around Psyr_1000

Experiment: KB with Auraptene 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_0999 and Psyr_1000 are separated by 293 nucleotidesPsyr_1000 and Psyr_1001 are separated by 213 nucleotides Psyr_0999: Psyr_0999 - luciferase, at 1,132,388 to 1,133,470 _0999 Psyr_1000: Psyr_1000 - transcriptional regulator, TetR family, at 1,133,764 to 1,134,492 _1000 Psyr_1001: Psyr_1001 - nucleoside-binding protein, at 1,134,706 to 1,135,797 _1001 Position (kb) 1133 1134 1135Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1132.863 kb on + strand, within Psyr_0999at 1132.863 kb on + strand, within Psyr_0999at 1132.864 kb on - strand, within Psyr_0999at 1132.864 kb on - strand, within Psyr_0999at 1133.043 kb on + strand, within Psyr_0999at 1133.043 kb on + strand, within Psyr_0999at 1133.044 kb on - strand, within Psyr_0999at 1133.044 kb on - strand, within Psyr_0999at 1133.403 kb on + strandat 1133.403 kb on + strandat 1133.403 kb on + strandat 1133.403 kb on + strandat 1133.403 kb on + strandat 1133.404 kb on - strandat 1133.404 kb on - strandat 1133.404 kb on - strandat 1133.404 kb on - strandat 1133.404 kb on - strandat 1133.404 kb on - strandat 1133.404 kb on - strandat 1133.549 kb on + strandat 1133.936 kb on + strand, within Psyr_1000at 1133.936 kb on + strand, within Psyr_1000at 1134.016 kb on + strand, within Psyr_1000at 1134.016 kb on + strand, within Psyr_1000at 1134.016 kb on + strand, within Psyr_1000at 1134.016 kb on + strand, within Psyr_1000at 1134.016 kb on + strand, within Psyr_1000at 1134.017 kb on - strand, within Psyr_1000at 1134.017 kb on - strand, within Psyr_1000at 1134.017 kb on - strand, within Psyr_1000at 1134.019 kb on + strand, within Psyr_1000at 1134.019 kb on + strand, within Psyr_1000at 1134.020 kb on - strand, within Psyr_1000at 1134.020 kb on - strand, within Psyr_1000at 1134.020 kb on - strand, within Psyr_1000at 1134.020 kb on - strand, within Psyr_1000at 1134.044 kb on - strand, within Psyr_1000at 1134.075 kb on - strand, within Psyr_1000at 1134.136 kb on + strand, within Psyr_1000at 1134.137 kb on - strand, within Psyr_1000at 1134.137 kb on - strand, within Psyr_1000at 1134.137 kb on - strand, within Psyr_1000at 1134.655 kb on + strandat 1134.705 kb on + strandat 1134.706 kb on - strandat 1134.709 kb on + strandat 1134.709 kb on + strandat 1134.709 kb on + strandat 1134.709 kb on + strandat 1134.709 kb on + strandat 1134.710 kb on - strandat 1134.710 kb on - strandat 1134.710 kb on - strandat 1134.710 kb on - strandat 1134.710 kb on - strandat 1134.820 kb on + strand, within Psyr_1001at 1134.820 kb on + strand, within Psyr_1001at 1134.820 kb on + strand, within Psyr_1001at 1134.820 kb on + strand, within Psyr_1001at 1134.820 kb on + strand, within Psyr_1001at 1134.820 kb on + strand, within Psyr_1001at 1134.820 kb on + strand, within Psyr_1001at 1134.820 kb on + strand, within Psyr_1001at 1134.820 kb on + strand, within Psyr_1001at 1134.820 kb on + strand, within Psyr_1001at 1134.820 kb on + strand, within Psyr_1001at 1134.820 kb on + strand, within Psyr_1001at 1134.821 kb on - strand, within Psyr_1001at 1134.821 kb on - strand, within Psyr_1001at 1134.821 kb on - strand, within Psyr_1001at 1134.821 kb on - strand, within Psyr_1001at 1134.821 kb on - strand, within Psyr_1001at 1134.821 kb on - strand, within Psyr_1001at 1134.821 kb on - strand, within Psyr_1001at 1134.821 kb on - strand, within Psyr_1001at 1135.114 kb on - strand, within Psyr_1001at 1135.118 kb on - strand, within Psyr_1001at 1135.118 kb on - strand, within Psyr_1001at 1135.218 kb on + strand, within Psyr_1001at 1135.219 kb on - strand, within Psyr_1001at 1135.279 kb on + strand, within Psyr_1001at 1135.279 kb on + strand, within Psyr_1001at 1135.279 kb on + strand, within Psyr_1001at 1135.280 kb on - strand, within Psyr_1001at 1135.280 kb on - strand, within Psyr_1001at 1135.280 kb on - strand, within Psyr_1001at 1135.280 kb on - strand, within Psyr_1001

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Auraptene 10 mM
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1,132,863 + Psyr_0999 0.44 -0.2
1,132,863 + Psyr_0999 0.44 -0.2
1,132,864 - Psyr_0999 0.44 -3.3
1,132,864 - Psyr_0999 0.44 -0.7
1,133,043 + Psyr_0999 0.60 +0.5
1,133,043 + Psyr_0999 0.60 -0.6
1,133,044 - Psyr_0999 0.61 -0.7
1,133,044 - Psyr_0999 0.61 -0.8
1,133,403 + -1.5
1,133,403 + +0.7
1,133,403 + -0.6
1,133,403 + -0.2
1,133,403 + +1.0
1,133,404 - +0.4
1,133,404 - -0.4
1,133,404 - -1.4
1,133,404 - -0.1
1,133,404 - -0.0
1,133,404 - +0.3
1,133,404 - +1.2
1,133,549 + +0.1
1,133,936 + Psyr_1000 0.24 +0.5
1,133,936 + Psyr_1000 0.24 -0.3
1,134,016 + Psyr_1000 0.35 +0.1
1,134,016 + Psyr_1000 0.35 +0.2
1,134,016 + Psyr_1000 0.35 -0.9
1,134,016 + Psyr_1000 0.35 +0.8
1,134,016 + Psyr_1000 0.35 -1.3
1,134,017 - Psyr_1000 0.35 -0.1
1,134,017 - Psyr_1000 0.35 +1.1
1,134,017 - Psyr_1000 0.35 +0.2
1,134,019 + Psyr_1000 0.35 +1.9
1,134,019 + Psyr_1000 0.35 -0.7
1,134,020 - Psyr_1000 0.35 -2.4
1,134,020 - Psyr_1000 0.35 -0.5
1,134,020 - Psyr_1000 0.35 +0.5
1,134,020 - Psyr_1000 0.35 +0.3
1,134,044 - Psyr_1000 0.38 -0.2
1,134,075 - Psyr_1000 0.43 +1.1
1,134,136 + Psyr_1000 0.51 +0.0
1,134,137 - Psyr_1000 0.51 -0.0
1,134,137 - Psyr_1000 0.51 +1.3
1,134,137 - Psyr_1000 0.51 -2.1
1,134,655 + -0.1
1,134,705 + -0.1
1,134,706 - -2.1
1,134,709 + -0.7
1,134,709 + +2.7
1,134,709 + -0.4
1,134,709 + -0.3
1,134,709 + +0.1
1,134,710 - +2.4
1,134,710 - +0.8
1,134,710 - +2.7
1,134,710 - -0.9
1,134,710 - -0.4
1,134,820 + Psyr_1001 0.10 -0.5
1,134,820 + Psyr_1001 0.10 +0.2
1,134,820 + Psyr_1001 0.10 -0.5
1,134,820 + Psyr_1001 0.10 -0.5
1,134,820 + Psyr_1001 0.10 -1.9
1,134,820 + Psyr_1001 0.10 -0.9
1,134,820 + Psyr_1001 0.10 -2.2
1,134,820 + Psyr_1001 0.10 -0.3
1,134,820 + Psyr_1001 0.10 -1.6
1,134,820 + Psyr_1001 0.10 -0.1
1,134,820 + Psyr_1001 0.10 +2.4
1,134,820 + Psyr_1001 0.10 +0.1
1,134,821 - Psyr_1001 0.11 -0.1
1,134,821 - Psyr_1001 0.11 -0.1
1,134,821 - Psyr_1001 0.11 -0.4
1,134,821 - Psyr_1001 0.11 -0.1
1,134,821 - Psyr_1001 0.11 +0.9
1,134,821 - Psyr_1001 0.11 +1.1
1,134,821 - Psyr_1001 0.11 -1.8
1,134,821 - Psyr_1001 0.11 -1.7
1,135,114 - Psyr_1001 0.37 +2.7
1,135,118 - Psyr_1001 0.38 +1.9
1,135,118 - Psyr_1001 0.38 -1.0
1,135,218 + Psyr_1001 0.47 -1.2
1,135,219 - Psyr_1001 0.47 -0.4
1,135,279 + Psyr_1001 0.52 +0.3
1,135,279 + Psyr_1001 0.52 +0.5
1,135,279 + Psyr_1001 0.52 -0.4
1,135,280 - Psyr_1001 0.53 -1.9
1,135,280 - Psyr_1001 0.53 -0.6
1,135,280 - Psyr_1001 0.53 +1.3
1,135,280 - Psyr_1001 0.53 -1.9

Or see this region's nucleotide sequence