Experiment: KB with Auraptene 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0272 and Psyr_0273 are separated by 94 nucleotides Psyr_0273 and Psyr_0274 overlap by 1 nucleotides Psyr_0274 and Psyr_0275 are separated by 234 nucleotides
Psyr_0272: Psyr_0272 - homoserine kinase, at 290,854 to 291,804
_0272
Psyr_0273: Psyr_0273 - Integrase, catalytic region, at 291,899 to 292,735
_0273
Psyr_0274: Psyr_0274 - ISPsy9, transposase OrfA, at 292,735 to 293,274
_0274
Psyr_0275: Psyr_0275 - Periplasmic solute binding protein, at 293,509 to 294,450
_0275
Position (kb) 291
292
293 Strain fitness (log2 ratio) -2
-1
0
1
2 at 291.781 kb on - strand at 291.833 kb on + strand at 291.833 kb on + strand at 291.833 kb on + strand at 291.833 kb on + strand at 291.834 kb on - strand at 291.893 kb on - strand at 291.901 kb on - strand at 291.901 kb on - strand at 291.901 kb on - strand at 293.492 kb on + strand at 293.492 kb on + strand at 293.492 kb on + strand at 293.493 kb on - strand at 293.493 kb on - strand at 293.493 kb on - strand at 293.493 kb on - strand at 293.493 kb on - strand at 293.493 kb on - strand at 293.493 kb on - strand at 293.518 kb on - strand at 293.518 kb on - strand at 293.591 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Auraptene 10 mM remove 291,781 - +2.0 291,833 + +0.1 291,833 + -1.4 291,833 + +0.4 291,833 + +1.1 291,834 - +1.5 291,893 - +0.3 291,901 - -0.4 291,901 - -1.7 291,901 - -0.8 293,492 + +0.9 293,492 + +0.4 293,492 + -0.0 293,493 - -0.3 293,493 - +1.6 293,493 - +0.5 293,493 - +1.0 293,493 - -1.5 293,493 - -2.0 293,493 - -0.2 293,518 - +1.7 293,518 - -0.0 293,591 + -0.1
Or see this region's nucleotide sequence