Experiment: KB with Dimethyl Sulfoxide 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2427 and Psyr_RNA41 are separated by 182 nucleotides Psyr_RNA41 and Psyr_2428 are separated by 658 nucleotides Psyr_2428 and Psyr_RNA42 are separated by 578 nucleotides Psyr_RNA42 and Psyr_2429 are separated by 134 nucleotides
Psyr_2427: Psyr_2427 - gamma-glutamylputrescine oxidase, at 2,807,649 to 2,808,932
_2427
Psyr_RNA41: Psyr_RNA41 - tRNA-Val, at 2,809,115 to 2,809,191
_RNA41
Psyr_2428: Psyr_2428 - GCN5-related N-acetyltransferase, at 2,809,850 to 2,810,326
_2428
Psyr_RNA42: Psyr_RNA42 - tRNA-Val, at 2,810,905 to 2,810,981
_RNA42
Psyr_2429: Psyr_2429 - conserved hypothetical protein, at 2,811,116 to 2,812,177
_2429
Position (kb)
2809
2810
2811 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2808.901 kb on + strand at 2808.901 kb on + strand at 2808.902 kb on - strand at 2808.904 kb on + strand at 2808.904 kb on + strand at 2808.905 kb on - strand at 2808.985 kb on + strand at 2808.986 kb on - strand at 2809.082 kb on + strand at 2809.327 kb on + strand at 2809.337 kb on + strand at 2809.338 kb on - strand at 2809.338 kb on - strand at 2809.364 kb on - strand at 2809.366 kb on + strand at 2809.367 kb on - strand at 2809.417 kb on + strand at 2809.427 kb on + strand at 2809.429 kb on + strand at 2809.430 kb on - strand at 2809.430 kb on - strand at 2809.430 kb on - strand at 2809.447 kb on + strand at 2809.448 kb on - strand at 2809.448 kb on - strand at 2809.448 kb on - strand at 2809.470 kb on - strand at 2809.470 kb on - strand at 2809.481 kb on + strand at 2809.493 kb on - strand at 2809.520 kb on + strand at 2809.520 kb on + strand at 2809.526 kb on + strand at 2809.548 kb on - strand at 2809.550 kb on + strand at 2809.551 kb on - strand at 2809.551 kb on - strand at 2809.551 kb on - strand at 2809.556 kb on + strand at 2809.556 kb on + strand at 2809.557 kb on - strand at 2809.565 kb on + strand at 2809.566 kb on - strand at 2809.597 kb on + strand at 2809.598 kb on - strand at 2809.598 kb on - strand at 2809.614 kb on + strand at 2809.647 kb on - strand at 2809.683 kb on + strand at 2809.684 kb on - strand at 2809.684 kb on - strand at 2809.684 kb on - strand at 2809.686 kb on + strand at 2809.686 kb on + strand at 2809.686 kb on + strand at 2809.686 kb on + strand at 2809.687 kb on - strand at 2809.690 kb on - strand at 2809.699 kb on - strand at 2809.712 kb on + strand at 2809.870 kb on - strand at 2809.918 kb on + strand, within Psyr_2428 at 2809.919 kb on - strand, within Psyr_2428 at 2810.028 kb on + strand, within Psyr_2428 at 2810.058 kb on - strand, within Psyr_2428 at 2810.076 kb on + strand, within Psyr_2428 at 2810.100 kb on - strand, within Psyr_2428 at 2810.100 kb on - strand, within Psyr_2428 at 2810.132 kb on - strand, within Psyr_2428 at 2810.151 kb on - strand, within Psyr_2428 at 2810.153 kb on + strand, within Psyr_2428 at 2810.154 kb on - strand, within Psyr_2428 at 2810.320 kb on + strand at 2810.373 kb on + strand at 2810.373 kb on + strand at 2810.374 kb on - strand at 2810.416 kb on + strand at 2810.485 kb on - strand at 2810.491 kb on - strand at 2810.647 kb on + strand at 2810.654 kb on + strand at 2810.654 kb on + strand at 2810.655 kb on - strand at 2810.655 kb on - strand at 2810.713 kb on - strand at 2810.722 kb on - strand at 2810.722 kb on - strand at 2810.725 kb on + strand at 2810.726 kb on - strand at 2810.837 kb on - strand at 2810.837 kb on - strand at 2811.060 kb on + strand at 2811.061 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Dimethyl Sulfoxide 1.25 vol% remove 2,808,901 + -1.0 2,808,901 + -2.0 2,808,902 - +1.6 2,808,904 + +1.0 2,808,904 + -0.7 2,808,905 - +1.3 2,808,985 + +0.6 2,808,986 - -0.2 2,809,082 + -1.0 2,809,327 + -0.4 2,809,337 + -1.1 2,809,338 - -2.0 2,809,338 - +1.4 2,809,364 - +0.0 2,809,366 + -1.4 2,809,367 - -1.0 2,809,417 + -0.1 2,809,427 + +1.2 2,809,429 + +0.4 2,809,430 - +0.4 2,809,430 - +0.9 2,809,430 - +0.0 2,809,447 + -0.7 2,809,448 - -0.6 2,809,448 - -1.5 2,809,448 - -0.0 2,809,470 - +0.4 2,809,470 - +0.9 2,809,481 + -0.5 2,809,493 - -0.6 2,809,520 + +1.3 2,809,520 + +0.5 2,809,526 + +3.2 2,809,548 - -0.3 2,809,550 + +0.7 2,809,551 - +0.3 2,809,551 - +0.7 2,809,551 - +0.3 2,809,556 + -1.5 2,809,556 + +0.0 2,809,557 - -0.2 2,809,565 + -1.1 2,809,566 - -0.7 2,809,597 + +3.3 2,809,598 - -0.7 2,809,598 - +2.0 2,809,614 + +1.7 2,809,647 - -2.1 2,809,683 + +0.7 2,809,684 - +0.4 2,809,684 - -0.7 2,809,684 - -0.4 2,809,686 + +1.3 2,809,686 + +2.1 2,809,686 + -1.7 2,809,686 + -1.5 2,809,687 - -0.6 2,809,690 - +0.2 2,809,699 - -3.7 2,809,712 + +1.3 2,809,870 - +0.4 2,809,918 + Psyr_2428 0.14 -0.2 2,809,919 - Psyr_2428 0.14 +0.3 2,810,028 + Psyr_2428 0.37 -0.2 2,810,058 - Psyr_2428 0.44 -1.6 2,810,076 + Psyr_2428 0.47 -3.2 2,810,100 - Psyr_2428 0.52 +0.2 2,810,100 - Psyr_2428 0.52 +1.3 2,810,132 - Psyr_2428 0.59 -0.8 2,810,151 - Psyr_2428 0.63 -0.7 2,810,153 + Psyr_2428 0.64 +0.6 2,810,154 - Psyr_2428 0.64 -0.8 2,810,320 + -0.3 2,810,373 + +2.9 2,810,373 + -1.6 2,810,374 - -0.4 2,810,416 + +0.2 2,810,485 - -0.7 2,810,491 - +1.0 2,810,647 + +0.3 2,810,654 + +0.3 2,810,654 + +0.0 2,810,655 - -1.1 2,810,655 - -0.6 2,810,713 - +0.7 2,810,722 - -2.7 2,810,722 - -1.4 2,810,725 + +0.8 2,810,726 - -1.4 2,810,837 - -1.2 2,810,837 - -0.9 2,811,060 + +0.8 2,811,061 - +1.7
Or see this region's nucleotide sequence