Experiment: KB with Dimethyl Sulfoxide 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_RNA26 and Psyr_RNA27 are separated by 21 nucleotides Psyr_RNA27 and Psyr_1589 are separated by 360 nucleotides Psyr_1589 and Psyr_1590 are separated by 125 nucleotides Psyr_1590 and Psyr_1591 are separated by 55 nucleotides Psyr_1591 and Psyr_1592 are separated by 32 nucleotides
Psyr_RNA26: Psyr_RNA26 - tRNA-Val, at 1,788,216 to 1,788,291
_RNA26
Psyr_RNA27: Psyr_RNA27 - tRNA-Asp, at 1,788,313 to 1,788,389
_RNA27
Psyr_1589: Psyr_1589 - conserved hypothetical protein, at 1,788,750 to 1,789,145
_1589
Psyr_1590: Psyr_1590 - L-carnitine dehydratase/bile acid-inducible protein F, at 1,789,271 to 1,790,461
_1590
Psyr_1591: Psyr_1591 - Glyoxalase/bleomycin resistance protein/dioxygenase, at 1,790,517 to 1,790,909
_1591
Psyr_1592: Psyr_1592 - conserved hypothetical protein, at 1,790,942 to 1,791,460
_1592
Position (kb)
1789
1790
1791 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1788.511 kb on - strand at 1788.545 kb on + strand at 1788.546 kb on - strand at 1788.550 kb on - strand at 1788.612 kb on + strand at 1788.627 kb on + strand at 1788.630 kb on + strand at 1788.686 kb on + strand at 1788.725 kb on + strand at 1788.750 kb on - strand at 1788.776 kb on - strand at 1788.823 kb on + strand, within Psyr_1589 at 1788.858 kb on - strand, within Psyr_1589 at 1788.897 kb on + strand, within Psyr_1589 at 1788.897 kb on + strand, within Psyr_1589 at 1788.898 kb on - strand, within Psyr_1589 at 1788.927 kb on + strand, within Psyr_1589 at 1788.927 kb on + strand, within Psyr_1589 at 1788.929 kb on + strand, within Psyr_1589 at 1788.929 kb on + strand, within Psyr_1589 at 1788.930 kb on - strand, within Psyr_1589 at 1789.069 kb on + strand, within Psyr_1589 at 1789.070 kb on - strand, within Psyr_1589 at 1789.080 kb on - strand, within Psyr_1589 at 1789.080 kb on - strand, within Psyr_1589 at 1789.080 kb on - strand, within Psyr_1589 at 1789.139 kb on + strand at 1789.542 kb on + strand, within Psyr_1590 at 1789.542 kb on + strand, within Psyr_1590 at 1789.542 kb on + strand, within Psyr_1590 at 1789.542 kb on + strand, within Psyr_1590 at 1789.857 kb on + strand, within Psyr_1590 at 1789.857 kb on + strand, within Psyr_1590 at 1789.857 kb on + strand, within Psyr_1590 at 1789.857 kb on + strand, within Psyr_1590 at 1789.857 kb on + strand, within Psyr_1590 at 1789.858 kb on - strand, within Psyr_1590 at 1789.858 kb on - strand, within Psyr_1590 at 1789.858 kb on - strand, within Psyr_1590 at 1789.872 kb on - strand, within Psyr_1590 at 1789.872 kb on - strand, within Psyr_1590 at 1790.004 kb on - strand, within Psyr_1590 at 1790.004 kb on - strand, within Psyr_1590 at 1790.004 kb on - strand, within Psyr_1590 at 1790.004 kb on - strand, within Psyr_1590 at 1790.032 kb on + strand, within Psyr_1590 at 1790.032 kb on + strand, within Psyr_1590 at 1790.033 kb on - strand, within Psyr_1590 at 1790.222 kb on + strand, within Psyr_1590 at 1790.223 kb on - strand, within Psyr_1590 at 1790.223 kb on - strand, within Psyr_1590 at 1790.329 kb on + strand, within Psyr_1590 at 1790.330 kb on - strand, within Psyr_1590 at 1790.355 kb on + strand at 1790.356 kb on - strand at 1790.356 kb on - strand at 1790.455 kb on + strand at 1790.455 kb on + strand at 1790.456 kb on - strand at 1790.495 kb on + strand at 1790.496 kb on - strand at 1790.543 kb on - strand at 1790.585 kb on - strand, within Psyr_1591 at 1790.844 kb on + strand, within Psyr_1591 at 1790.844 kb on + strand, within Psyr_1591 at 1790.844 kb on + strand, within Psyr_1591 at 1790.844 kb on + strand, within Psyr_1591 at 1790.844 kb on + strand, within Psyr_1591 at 1790.844 kb on + strand, within Psyr_1591 at 1790.844 kb on + strand, within Psyr_1591 at 1790.844 kb on + strand, within Psyr_1591 at 1790.845 kb on - strand, within Psyr_1591 at 1790.845 kb on - strand, within Psyr_1591 at 1790.845 kb on - strand, within Psyr_1591 at 1790.845 kb on - strand, within Psyr_1591 at 1790.845 kb on - strand, within Psyr_1591 at 1790.845 kb on - strand, within Psyr_1591 at 1790.845 kb on - strand, within Psyr_1591 at 1790.865 kb on - strand, within Psyr_1591 at 1790.907 kb on + strand at 1790.908 kb on - strand at 1791.012 kb on + strand, within Psyr_1592 at 1791.012 kb on + strand, within Psyr_1592 at 1791.012 kb on + strand, within Psyr_1592 at 1791.012 kb on + strand, within Psyr_1592 at 1791.012 kb on + strand, within Psyr_1592 at 1791.012 kb on + strand, within Psyr_1592 at 1791.012 kb on + strand, within Psyr_1592 at 1791.012 kb on + strand, within Psyr_1592 at 1791.012 kb on + strand, within Psyr_1592 at 1791.012 kb on + strand, within Psyr_1592 at 1791.012 kb on + strand, within Psyr_1592 at 1791.012 kb on + strand, within Psyr_1592 at 1791.012 kb on + strand, within Psyr_1592 at 1791.012 kb on + strand, within Psyr_1592 at 1791.012 kb on + strand, within Psyr_1592 at 1791.012 kb on + strand, within Psyr_1592 at 1791.013 kb on - strand, within Psyr_1592 at 1791.013 kb on - strand, within Psyr_1592 at 1791.013 kb on - strand, within Psyr_1592 at 1791.013 kb on - strand, within Psyr_1592 at 1791.013 kb on - strand, within Psyr_1592 at 1791.013 kb on - strand, within Psyr_1592 at 1791.013 kb on - strand, within Psyr_1592 at 1791.013 kb on - strand, within Psyr_1592 at 1791.013 kb on - strand, within Psyr_1592 at 1791.013 kb on - strand, within Psyr_1592 at 1791.013 kb on - strand, within Psyr_1592 at 1791.013 kb on - strand, within Psyr_1592 at 1791.013 kb on - strand, within Psyr_1592 at 1791.013 kb on - strand, within Psyr_1592 at 1791.086 kb on + strand, within Psyr_1592 at 1791.086 kb on + strand, within Psyr_1592 at 1791.086 kb on + strand, within Psyr_1592 at 1791.087 kb on - strand, within Psyr_1592 at 1791.087 kb on - strand, within Psyr_1592 at 1791.087 kb on - strand, within Psyr_1592 at 1791.162 kb on + strand, within Psyr_1592 at 1791.162 kb on + strand, within Psyr_1592 at 1791.162 kb on + strand, within Psyr_1592 at 1791.162 kb on + strand, within Psyr_1592 at 1791.163 kb on - strand, within Psyr_1592 at 1791.163 kb on - strand, within Psyr_1592 at 1791.163 kb on - strand, within Psyr_1592 at 1791.163 kb on - strand, within Psyr_1592 at 1791.357 kb on + strand, within Psyr_1592 at 1791.358 kb on - strand, within Psyr_1592 at 1791.358 kb on - strand, within Psyr_1592
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Dimethyl Sulfoxide 1.25 vol% remove 1,788,511 - -1.0 1,788,545 + +2.0 1,788,546 - -0.2 1,788,550 - -0.5 1,788,612 + +0.1 1,788,627 + -0.8 1,788,630 + -0.2 1,788,686 + +1.3 1,788,725 + -0.1 1,788,750 - -0.3 1,788,776 - -2.7 1,788,823 + Psyr_1589 0.18 +0.9 1,788,858 - Psyr_1589 0.27 +1.7 1,788,897 + Psyr_1589 0.37 -0.1 1,788,897 + Psyr_1589 0.37 +0.9 1,788,898 - Psyr_1589 0.37 -0.7 1,788,927 + Psyr_1589 0.45 +0.9 1,788,927 + Psyr_1589 0.45 -0.9 1,788,929 + Psyr_1589 0.45 -1.1 1,788,929 + Psyr_1589 0.45 +0.8 1,788,930 - Psyr_1589 0.45 +0.5 1,789,069 + Psyr_1589 0.81 +0.8 1,789,070 - Psyr_1589 0.81 -2.6 1,789,080 - Psyr_1589 0.83 +0.8 1,789,080 - Psyr_1589 0.83 -1.2 1,789,080 - Psyr_1589 0.83 +3.3 1,789,139 + +1.3 1,789,542 + Psyr_1590 0.23 -0.3 1,789,542 + Psyr_1590 0.23 -1.5 1,789,542 + Psyr_1590 0.23 -0.4 1,789,542 + Psyr_1590 0.23 -1.3 1,789,857 + Psyr_1590 0.49 -0.8 1,789,857 + Psyr_1590 0.49 -0.3 1,789,857 + Psyr_1590 0.49 +1.3 1,789,857 + Psyr_1590 0.49 +0.7 1,789,857 + Psyr_1590 0.49 +0.2 1,789,858 - Psyr_1590 0.49 +0.7 1,789,858 - Psyr_1590 0.49 -1.4 1,789,858 - Psyr_1590 0.49 +0.3 1,789,872 - Psyr_1590 0.50 +0.7 1,789,872 - Psyr_1590 0.50 +0.4 1,790,004 - Psyr_1590 0.62 -1.0 1,790,004 - Psyr_1590 0.62 +0.1 1,790,004 - Psyr_1590 0.62 -0.1 1,790,004 - Psyr_1590 0.62 -0.1 1,790,032 + Psyr_1590 0.64 +0.4 1,790,032 + Psyr_1590 0.64 -1.2 1,790,033 - Psyr_1590 0.64 +0.3 1,790,222 + Psyr_1590 0.80 -1.4 1,790,223 - Psyr_1590 0.80 +1.7 1,790,223 - Psyr_1590 0.80 -0.5 1,790,329 + Psyr_1590 0.89 -0.1 1,790,330 - Psyr_1590 0.89 +0.8 1,790,355 + -0.3 1,790,356 - -0.3 1,790,356 - +0.2 1,790,455 + +0.1 1,790,455 + +2.3 1,790,456 - +0.6 1,790,495 + +1.4 1,790,496 - +1.3 1,790,543 - +2.9 1,790,585 - Psyr_1591 0.17 +1.3 1,790,844 + Psyr_1591 0.83 -0.0 1,790,844 + Psyr_1591 0.83 -0.2 1,790,844 + Psyr_1591 0.83 +0.2 1,790,844 + Psyr_1591 0.83 +1.2 1,790,844 + Psyr_1591 0.83 +0.9 1,790,844 + Psyr_1591 0.83 +0.7 1,790,844 + Psyr_1591 0.83 +0.9 1,790,844 + Psyr_1591 0.83 +0.8 1,790,845 - Psyr_1591 0.83 +1.3 1,790,845 - Psyr_1591 0.83 -0.6 1,790,845 - Psyr_1591 0.83 +0.3 1,790,845 - Psyr_1591 0.83 -2.7 1,790,845 - Psyr_1591 0.83 +0.9 1,790,845 - Psyr_1591 0.83 -2.2 1,790,845 - Psyr_1591 0.83 +0.9 1,790,865 - Psyr_1591 0.89 -0.6 1,790,907 + +1.2 1,790,908 - -0.4 1,791,012 + Psyr_1592 0.13 +0.0 1,791,012 + Psyr_1592 0.13 -0.7 1,791,012 + Psyr_1592 0.13 +0.0 1,791,012 + Psyr_1592 0.13 +1.0 1,791,012 + Psyr_1592 0.13 +0.2 1,791,012 + Psyr_1592 0.13 +0.1 1,791,012 + Psyr_1592 0.13 -2.3 1,791,012 + Psyr_1592 0.13 -1.6 1,791,012 + Psyr_1592 0.13 -2.2 1,791,012 + Psyr_1592 0.13 +0.1 1,791,012 + Psyr_1592 0.13 -0.7 1,791,012 + Psyr_1592 0.13 -0.6 1,791,012 + Psyr_1592 0.13 -0.8 1,791,012 + Psyr_1592 0.13 +0.8 1,791,012 + Psyr_1592 0.13 -0.5 1,791,012 + Psyr_1592 0.13 +1.1 1,791,013 - Psyr_1592 0.14 -0.3 1,791,013 - Psyr_1592 0.14 -0.8 1,791,013 - Psyr_1592 0.14 -0.1 1,791,013 - Psyr_1592 0.14 -3.0 1,791,013 - Psyr_1592 0.14 -0.1 1,791,013 - Psyr_1592 0.14 -0.3 1,791,013 - Psyr_1592 0.14 +2.0 1,791,013 - Psyr_1592 0.14 -1.3 1,791,013 - Psyr_1592 0.14 -0.2 1,791,013 - Psyr_1592 0.14 -0.2 1,791,013 - Psyr_1592 0.14 -0.5 1,791,013 - Psyr_1592 0.14 -2.2 1,791,013 - Psyr_1592 0.14 +3.9 1,791,013 - Psyr_1592 0.14 -1.0 1,791,086 + Psyr_1592 0.28 +0.1 1,791,086 + Psyr_1592 0.28 +1.7 1,791,086 + Psyr_1592 0.28 -2.8 1,791,087 - Psyr_1592 0.28 -0.9 1,791,087 - Psyr_1592 0.28 -2.3 1,791,087 - Psyr_1592 0.28 -0.6 1,791,162 + Psyr_1592 0.42 +1.3 1,791,162 + Psyr_1592 0.42 +1.7 1,791,162 + Psyr_1592 0.42 +0.6 1,791,162 + Psyr_1592 0.42 +0.9 1,791,163 - Psyr_1592 0.43 +1.1 1,791,163 - Psyr_1592 0.43 +0.2 1,791,163 - Psyr_1592 0.43 +0.4 1,791,163 - Psyr_1592 0.43 +0.2 1,791,357 + Psyr_1592 0.80 +2.9 1,791,358 - Psyr_1592 0.80 -0.5 1,791,358 - Psyr_1592 0.80 -0.9
Or see this region's nucleotide sequence