Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_26730 and PS417_26735 are separated by 9 nucleotides PS417_26735 and PS417_26740 are separated by 207 nucleotides PS417_26740 and PS417_26745 are separated by 7 nucleotides PS417_26745 and PS417_26750 are separated by 21 nucleotides
PS417_26730: PS417_26730 - pyrroline-5-carboxylate reductase, at 5,776,531 to 5,777,349
_26730
PS417_26735: PS417_26735 - membrane protein, at 5,777,359 to 5,777,949
_26735
PS417_26740: PS417_26740 - homoserine acetyltransferase, at 5,778,157 to 5,779,296
_26740
PS417_26745: PS417_26745 - methionine biosynthesis protein MetW, at 5,779,304 to 5,779,924
_26745
PS417_26750: PS417_26750 - homoserine acetyltransferase, at 5,779,946 to 5,780,380
_26750
Position (kb) 5778
5779
5780 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5777.347 kb on + strand at 5777.347 kb on + strand at 5777.947 kb on + strand at 5777.998 kb on + strand at 5777.998 kb on + strand at 5778.063 kb on - strand at 5778.122 kb on + strand at 5778.128 kb on + strand at 5778.128 kb on + strand at 5778.129 kb on - strand at 5778.129 kb on - strand at 5778.259 kb on + strand at 5778.271 kb on + strand, within PS417_26740 at 5778.271 kb on + strand, within PS417_26740 at 5778.272 kb on - strand, within PS417_26740 at 5778.272 kb on - strand, within PS417_26740 at 5778.665 kb on - strand, within PS417_26740 at 5778.850 kb on - strand, within PS417_26740 at 5778.856 kb on + strand, within PS417_26740 at 5778.856 kb on + strand, within PS417_26740 at 5778.856 kb on + strand, within PS417_26740 at 5778.857 kb on - strand, within PS417_26740 at 5778.955 kb on + strand, within PS417_26740 at 5779.033 kb on + strand, within PS417_26740 at 5779.196 kb on - strand at 5779.196 kb on - strand at 5779.252 kb on + strand at 5779.253 kb on - strand at 5779.273 kb on + strand at 5779.274 kb on - strand at 5779.709 kb on + strand, within PS417_26745 at 5779.719 kb on - strand, within PS417_26745 at 5779.719 kb on - strand, within PS417_26745 at 5779.719 kb on - strand, within PS417_26745 at 5779.719 kb on - strand, within PS417_26745 at 5779.841 kb on - strand, within PS417_26745 at 5779.845 kb on + strand, within PS417_26745 at 5779.845 kb on + strand, within PS417_26745 at 5779.845 kb on + strand, within PS417_26745 at 5779.845 kb on + strand, within PS417_26745 at 5779.922 kb on + strand at 5779.923 kb on - strand at 5780.012 kb on - strand, within PS417_26750 at 5780.013 kb on + strand, within PS417_26750 at 5780.013 kb on + strand, within PS417_26750 at 5780.014 kb on - strand, within PS417_26750 at 5780.057 kb on + strand, within PS417_26750 at 5780.058 kb on - strand, within PS417_26750 at 5780.115 kb on + strand, within PS417_26750
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x remove 5,777,347 + -0.5 5,777,347 + +1.5 5,777,947 + -0.1 5,777,998 + +0.2 5,777,998 + +1.0 5,778,063 - +1.8 5,778,122 + +0.0 5,778,128 + +0.1 5,778,128 + +0.0 5,778,129 - +0.8 5,778,129 - -1.1 5,778,259 + -0.6 5,778,271 + PS417_26740 0.10 -1.1 5,778,271 + PS417_26740 0.10 -1.1 5,778,272 - PS417_26740 0.10 -3.1 5,778,272 - PS417_26740 0.10 -1.2 5,778,665 - PS417_26740 0.45 +0.8 5,778,850 - PS417_26740 0.61 -1.5 5,778,856 + PS417_26740 0.61 -2.0 5,778,856 + PS417_26740 0.61 -1.5 5,778,856 + PS417_26740 0.61 -1.3 5,778,857 - PS417_26740 0.61 -0.7 5,778,955 + PS417_26740 0.70 -1.0 5,779,033 + PS417_26740 0.77 -1.5 5,779,196 - +0.1 5,779,196 - -0.7 5,779,252 + -0.1 5,779,253 - -1.4 5,779,273 + -0.6 5,779,274 - -1.4 5,779,709 + PS417_26745 0.65 -1.3 5,779,719 - PS417_26745 0.67 +0.4 5,779,719 - PS417_26745 0.67 -0.8 5,779,719 - PS417_26745 0.67 -1.2 5,779,719 - PS417_26745 0.67 -1.2 5,779,841 - PS417_26745 0.86 -1.8 5,779,845 + PS417_26745 0.87 -1.9 5,779,845 + PS417_26745 0.87 -0.9 5,779,845 + PS417_26745 0.87 +0.2 5,779,845 + PS417_26745 0.87 -1.9 5,779,922 + -0.4 5,779,923 - +0.6 5,780,012 - PS417_26750 0.15 -0.0 5,780,013 + PS417_26750 0.15 +2.2 5,780,013 + PS417_26750 0.15 -0.6 5,780,014 - PS417_26750 0.16 +1.6 5,780,057 + PS417_26750 0.26 +0.2 5,780,058 - PS417_26750 0.26 -0.1 5,780,115 + PS417_26750 0.39 +0.2
Or see this region's nucleotide sequence