Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_27570 and PS417_27580 are separated by 418 nucleotides PS417_27580 and PS417_27585 are separated by 3 nucleotides PS417_27585 and PS417_27590 are separated by 13 nucleotides
PS417_27570: PS417_27570 - frataxin, at 5,948,399 to 5,948,731
_27570
PS417_27580: PS417_27580 - diaminopimelate decarboxylase, at 5,949,150 to 5,950,397
_27580
PS417_27585: PS417_27585 - diaminopimelate epimerase, at 5,950,401 to 5,951,231
_27585
PS417_27590: PS417_27590 - hypothetical protein, at 5,951,245 to 5,951,961
_27590
Position (kb)
5949
5950
5951 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5948.321 kb on + strand at 5948.322 kb on - strand at 5948.352 kb on - strand at 5948.852 kb on + strand at 5948.853 kb on - strand at 5948.946 kb on + strand at 5949.132 kb on + strand at 5950.395 kb on + strand at 5950.540 kb on + strand, within PS417_27585 at 5950.540 kb on + strand, within PS417_27585 at 5950.821 kb on + strand, within PS417_27585 at 5951.208 kb on + strand at 5951.243 kb on + strand at 5951.244 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 5,948,321 + -0.8 5,948,322 - -1.1 5,948,352 - -0.4 5,948,852 + -0.0 5,948,853 - -1.4 5,948,946 + +0.5 5,949,132 + +2.5 5,950,395 + +0.3 5,950,540 + PS417_27585 0.17 +0.8 5,950,540 + PS417_27585 0.17 -1.9 5,950,821 + PS417_27585 0.51 -1.4 5,951,208 + -0.8 5,951,243 + -0.7 5,951,244 - -0.4
Or see this region's nucleotide sequence