Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_27515 and PS417_27520 are separated by 5 nucleotides PS417_27520 and PS417_27525 are separated by 18 nucleotides PS417_27525 and PS417_27530 overlap by 4 nucleotides
PS417_27515: PS417_27515 - heme biosynthesis protein HemY, at 5,936,205 to 5,937,446
_27515
PS417_27520: PS417_27520 - uroporphyrin-III methyltransferase, at 5,937,452 to 5,938,564
_27520
PS417_27525: PS417_27525 - uroporphyrinogen-III synthase, at 5,938,583 to 5,939,341
_27525
PS417_27530: PS417_27530 - porphobilinogen deaminase, at 5,939,338 to 5,940,279
_27530
Position (kb) 5937
5938
5939 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5936.521 kb on + strand, within PS417_27515 at 5936.521 kb on + strand, within PS417_27515 at 5936.522 kb on - strand, within PS417_27515 at 5936.620 kb on + strand, within PS417_27515 at 5936.621 kb on - strand, within PS417_27515 at 5936.792 kb on + strand, within PS417_27515 at 5937.079 kb on + strand, within PS417_27515 at 5937.082 kb on + strand, within PS417_27515 at 5937.297 kb on + strand, within PS417_27515 at 5937.349 kb on + strand at 5937.353 kb on + strand at 5937.353 kb on + strand at 5937.353 kb on + strand at 5937.353 kb on + strand at 5937.354 kb on - strand at 5937.354 kb on - strand at 5937.354 kb on - strand at 5937.433 kb on + strand at 5937.433 kb on + strand at 5937.434 kb on - strand at 5937.434 kb on - strand at 5937.519 kb on + strand at 5937.519 kb on + strand at 5937.519 kb on + strand at 5937.520 kb on - strand at 5937.681 kb on + strand, within PS417_27520 at 5937.906 kb on + strand, within PS417_27520 at 5937.966 kb on + strand, within PS417_27520 at 5937.966 kb on + strand, within PS417_27520 at 5937.968 kb on + strand, within PS417_27520 at 5937.969 kb on - strand, within PS417_27520 at 5938.183 kb on + strand, within PS417_27520 at 5938.543 kb on + strand at 5938.566 kb on + strand at 5938.567 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 5,936,521 + PS417_27515 0.25 -1.0 5,936,521 + PS417_27515 0.25 -1.9 5,936,522 - PS417_27515 0.26 -0.9 5,936,620 + PS417_27515 0.33 -0.3 5,936,621 - PS417_27515 0.33 +0.4 5,936,792 + PS417_27515 0.47 -0.4 5,937,079 + PS417_27515 0.70 -1.1 5,937,082 + PS417_27515 0.71 -1.1 5,937,297 + PS417_27515 0.88 -1.1 5,937,349 + -0.4 5,937,353 + -1.2 5,937,353 + -1.0 5,937,353 + -1.5 5,937,353 + -2.9 5,937,354 - -1.7 5,937,354 - -1.2 5,937,354 - -1.9 5,937,433 + -1.4 5,937,433 + -2.1 5,937,434 - -1.0 5,937,434 - -1.7 5,937,519 + +0.0 5,937,519 + -0.7 5,937,519 + -1.1 5,937,520 - -0.6 5,937,681 + PS417_27520 0.21 -0.3 5,937,906 + PS417_27520 0.41 -2.4 5,937,966 + PS417_27520 0.46 -1.0 5,937,966 + PS417_27520 0.46 -1.7 5,937,968 + PS417_27520 0.46 -0.4 5,937,969 - PS417_27520 0.46 -2.7 5,938,183 + PS417_27520 0.66 -2.5 5,938,543 + -0.5 5,938,566 + -0.8 5,938,567 - -0.3
Or see this region's nucleotide sequence