Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_23900 and PS417_23905 are separated by 2 nucleotides PS417_23905 and PS417_23910 are separated by 307 nucleotides PS417_23910 and PS417_23915 overlap by 4 nucleotides
PS417_23900: PS417_23900 - poly(A) polymerase, at 5,172,344 to 5,173,747
_23900
PS417_23905: PS417_23905 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase, at 5,173,750 to 5,174,235
_23905
PS417_23910: PS417_23910 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 5,174,543 to 5,175,343
_23910
PS417_23915: PS417_23915 - pantoate--beta-alanine ligase, at 5,175,340 to 5,176,191
_23915
Position (kb)
5174
5175
5176 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 5173.762 kb on + strand at 5174.398 kb on + strand at 5174.398 kb on + strand at 5174.399 kb on - strand at 5174.400 kb on + strand at 5174.450 kb on + strand at 5174.450 kb on + strand at 5174.450 kb on + strand at 5174.451 kb on - strand at 5174.451 kb on - strand at 5174.451 kb on - strand at 5174.452 kb on + strand at 5174.453 kb on - strand at 5174.453 kb on - strand at 5174.453 kb on - strand at 5174.453 kb on - strand at 5174.453 kb on - strand at 5174.481 kb on + strand at 5174.483 kb on + strand at 5174.997 kb on + strand, within PS417_23910 at 5174.997 kb on + strand, within PS417_23910 at 5174.997 kb on + strand, within PS417_23910 at 5174.997 kb on + strand, within PS417_23910 at 5174.998 kb on - strand, within PS417_23910 at 5174.998 kb on - strand, within PS417_23910 at 5174.998 kb on - strand, within PS417_23910 at 5174.998 kb on - strand, within PS417_23910 at 5175.115 kb on + strand, within PS417_23910 at 5175.183 kb on + strand, within PS417_23910 at 5175.284 kb on + strand at 5175.550 kb on + strand, within PS417_23915 at 5175.634 kb on + strand, within PS417_23915 at 5175.797 kb on - strand, within PS417_23915 at 5175.872 kb on + strand, within PS417_23915 at 5176.223 kb on + strand at 5176.276 kb on + strand at 5176.276 kb on + strand at 5176.288 kb on - strand at 5176.288 kb on - strand at 5176.298 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 5,173,762 + -0.9 5,174,398 + +0.9 5,174,398 + -1.3 5,174,399 - +0.9 5,174,400 + -0.1 5,174,450 + +0.0 5,174,450 + -1.2 5,174,450 + -0.3 5,174,451 - -2.3 5,174,451 - -2.1 5,174,451 - -2.3 5,174,452 + -0.2 5,174,453 - -1.1 5,174,453 - -1.0 5,174,453 - -1.1 5,174,453 - -0.6 5,174,453 - -0.6 5,174,481 + +0.1 5,174,483 + -0.8 5,174,997 + PS417_23910 0.57 -1.1 5,174,997 + PS417_23910 0.57 -3.1 5,174,997 + PS417_23910 0.57 -3.1 5,174,997 + PS417_23910 0.57 -5.2 5,174,998 - PS417_23910 0.57 -2.7 5,174,998 - PS417_23910 0.57 -4.4 5,174,998 - PS417_23910 0.57 -2.8 5,174,998 - PS417_23910 0.57 -1.5 5,175,115 + PS417_23910 0.71 -2.0 5,175,183 + PS417_23910 0.80 -1.0 5,175,284 + -2.2 5,175,550 + PS417_23915 0.25 -0.9 5,175,634 + PS417_23915 0.35 -0.8 5,175,797 - PS417_23915 0.54 -0.5 5,175,872 + PS417_23915 0.62 -0.8 5,176,223 + -1.2 5,176,276 + +0.1 5,176,276 + -0.3 5,176,288 - -0.1 5,176,288 - +0.2 5,176,298 + +0.1
Or see this region's nucleotide sequence