Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_22190 and PS417_22195 are separated by 127 nucleotides PS417_22195 and PS417_22200 are separated by 79 nucleotides
PS417_22190: PS417_22190 - diguanylate cyclase, at 4,805,650 to 4,807,701
_22190
PS417_22195: PS417_22195 - peptidase, at 4,807,829 to 4,809,172
_22195
PS417_22200: PS417_22200 - hypothetical protein, at 4,809,252 to 4,813,466
_22200
Position (kb)
4807
4808
4809
4810 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 4806.880 kb on + strand, within PS417_22190 at 4806.881 kb on - strand, within PS417_22190 at 4806.974 kb on - strand, within PS417_22190 at 4807.320 kb on + strand at 4807.524 kb on + strand at 4807.582 kb on + strand at 4807.583 kb on - strand at 4807.583 kb on - strand at 4807.583 kb on - strand at 4807.583 kb on - strand at 4807.624 kb on + strand at 4807.734 kb on - strand at 4807.837 kb on + strand at 4807.838 kb on - strand at 4808.163 kb on - strand, within PS417_22195 at 4808.163 kb on - strand, within PS417_22195 at 4808.312 kb on - strand, within PS417_22195 at 4808.513 kb on + strand, within PS417_22195 at 4808.646 kb on - strand, within PS417_22195 at 4808.822 kb on + strand, within PS417_22195 at 4809.017 kb on + strand, within PS417_22195 at 4809.188 kb on + strand at 4809.266 kb on + strand at 4809.305 kb on + strand at 4809.305 kb on + strand at 4809.329 kb on + strand at 4809.330 kb on - strand at 4809.330 kb on - strand at 4809.388 kb on + strand at 4809.426 kb on + strand at 4809.503 kb on - strand at 4809.700 kb on - strand, within PS417_22200 at 4809.727 kb on + strand, within PS417_22200 at 4809.727 kb on + strand, within PS417_22200 at 4809.728 kb on - strand, within PS417_22200 at 4809.728 kb on - strand, within PS417_22200 at 4809.728 kb on - strand, within PS417_22200 at 4809.883 kb on + strand at 4809.883 kb on + strand, within PS417_22200 at 4809.883 kb on + strand, within PS417_22200 at 4809.884 kb on - strand, within PS417_22200 at 4809.884 kb on - strand, within PS417_22200 at 4809.884 kb on - strand, within PS417_22200 at 4809.884 kb on - strand, within PS417_22200 at 4809.884 kb on - strand, within PS417_22200 at 4809.884 kb on - strand, within PS417_22200 at 4809.973 kb on + strand at 4809.974 kb on - strand, within PS417_22200 at 4809.974 kb on - strand, within PS417_22200 at 4809.974 kb on - strand, within PS417_22200 at 4810.012 kb on + strand, within PS417_22200 at 4810.013 kb on - strand, within PS417_22200 at 4810.044 kb on + strand, within PS417_22200 at 4810.045 kb on - strand, within PS417_22200 at 4810.045 kb on - strand, within PS417_22200 at 4810.108 kb on + strand, within PS417_22200 at 4810.108 kb on + strand, within PS417_22200 at 4810.108 kb on + strand at 4810.108 kb on + strand, within PS417_22200 at 4810.109 kb on - strand, within PS417_22200 at 4810.109 kb on - strand, within PS417_22200
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 4,806,880 + PS417_22190 0.60 +1.1 4,806,881 - PS417_22190 0.60 +0.2 4,806,974 - PS417_22190 0.65 +0.6 4,807,320 + -0.2 4,807,524 + -0.0 4,807,582 + -0.5 4,807,583 - +0.3 4,807,583 - -0.2 4,807,583 - +0.1 4,807,583 - -1.7 4,807,624 + -0.2 4,807,734 - +0.0 4,807,837 + +0.4 4,807,838 - -3.3 4,808,163 - PS417_22195 0.25 -1.7 4,808,163 - PS417_22195 0.25 -4.9 4,808,312 - PS417_22195 0.36 -3.8 4,808,513 + PS417_22195 0.51 -4.4 4,808,646 - PS417_22195 0.61 -2.0 4,808,822 + PS417_22195 0.74 -1.3 4,809,017 + PS417_22195 0.88 -4.5 4,809,188 + +0.3 4,809,266 + +1.4 4,809,305 + -0.3 4,809,305 + +0.1 4,809,329 + -0.2 4,809,330 - -1.2 4,809,330 - +0.6 4,809,388 + +0.1 4,809,426 + +0.2 4,809,503 - -1.4 4,809,700 - PS417_22200 0.11 +0.2 4,809,727 + PS417_22200 0.11 -0.5 4,809,727 + PS417_22200 0.11 +3.2 4,809,728 - PS417_22200 0.11 +0.5 4,809,728 - PS417_22200 0.11 -0.4 4,809,728 - PS417_22200 0.11 -1.7 4,809,883 + +1.8 4,809,883 + PS417_22200 0.15 +0.3 4,809,883 + PS417_22200 0.15 -0.2 4,809,884 - PS417_22200 0.15 -0.5 4,809,884 - PS417_22200 0.15 +0.7 4,809,884 - PS417_22200 0.15 +0.7 4,809,884 - PS417_22200 0.15 +0.5 4,809,884 - PS417_22200 0.15 -0.0 4,809,884 - PS417_22200 0.15 +1.1 4,809,973 + +2.4 4,809,974 - PS417_22200 0.17 +2.8 4,809,974 - PS417_22200 0.17 -0.4 4,809,974 - PS417_22200 0.17 -0.7 4,810,012 + PS417_22200 0.18 -1.2 4,810,013 - PS417_22200 0.18 +0.1 4,810,044 + PS417_22200 0.19 -0.7 4,810,045 - PS417_22200 0.19 -1.2 4,810,045 - PS417_22200 0.19 -0.0 4,810,108 + PS417_22200 0.20 -0.6 4,810,108 + PS417_22200 0.20 -1.3 4,810,108 + +0.4 4,810,108 + PS417_22200 0.20 +0.3 4,810,109 - PS417_22200 0.20 +0.7 4,810,109 - PS417_22200 0.20 -0.0
Or see this region's nucleotide sequence