Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02200 and PS417_02205 are separated by 138 nucleotides PS417_02205 and PS417_02210 are separated by 72 nucleotides
PS417_02200: PS417_02200 - lactate dehydrogenase, at 495,965 to 496,801
_02200
PS417_02205: PS417_02205 - peptide methionine sulfoxide reductase, at 496,940 to 497,587
_02205
PS417_02210: PS417_02210 - diguanylate cyclase, at 497,660 to 500,353
_02210
Position (kb) 496
497
498 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 496.810 kb on + strand at 496.811 kb on - strand at 496.863 kb on - strand at 496.863 kb on - strand at 496.863 kb on - strand at 496.863 kb on - strand at 496.870 kb on + strand at 496.873 kb on + strand at 496.874 kb on - strand at 496.941 kb on + strand at 496.981 kb on - strand at 497.005 kb on - strand, within PS417_02205 at 497.005 kb on - strand, within PS417_02205 at 497.016 kb on + strand, within PS417_02205 at 497.031 kb on + strand, within PS417_02205 at 497.031 kb on + strand, within PS417_02205 at 497.101 kb on - strand, within PS417_02205 at 497.151 kb on + strand, within PS417_02205 at 497.152 kb on - strand, within PS417_02205 at 497.166 kb on + strand, within PS417_02205 at 497.269 kb on - strand, within PS417_02205 at 497.269 kb on - strand, within PS417_02205 at 497.269 kb on - strand, within PS417_02205 at 497.269 kb on - strand, within PS417_02205 at 497.322 kb on + strand, within PS417_02205 at 497.323 kb on - strand, within PS417_02205 at 497.822 kb on - strand at 497.941 kb on - strand, within PS417_02210 at 497.941 kb on - strand, within PS417_02210
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 496,810 + -0.8 496,811 - +0.7 496,863 - -0.6 496,863 - -0.5 496,863 - +0.2 496,863 - -0.5 496,870 + +0.7 496,873 + +0.2 496,874 - +1.3 496,941 + -0.6 496,981 - +0.9 497,005 - PS417_02205 0.10 +0.2 497,005 - PS417_02205 0.10 +0.7 497,016 + PS417_02205 0.12 +0.7 497,031 + PS417_02205 0.14 +1.4 497,031 + PS417_02205 0.14 +0.8 497,101 - PS417_02205 0.25 +0.7 497,151 + PS417_02205 0.33 +1.9 497,152 - PS417_02205 0.33 -0.1 497,166 + PS417_02205 0.35 +1.1 497,269 - PS417_02205 0.51 +1.0 497,269 - PS417_02205 0.51 +0.9 497,269 - PS417_02205 0.51 +0.2 497,269 - PS417_02205 0.51 -1.8 497,322 + PS417_02205 0.59 +1.2 497,323 - PS417_02205 0.59 +0.9 497,822 - -1.1 497,941 - PS417_02210 0.10 -3.3 497,941 - PS417_02210 0.10 +2.5
Or see this region's nucleotide sequence