Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_19695 and PS417_19700 overlap by 4 nucleotides PS417_19700 and PS417_19705 are separated by 106 nucleotides PS417_19705 and PS417_19710 are separated by 12 nucleotides
PS417_19695: PS417_19695 - flagellar biosynthesis sigma factor, at 4,280,216 to 4,280,956
_19695
PS417_19700: PS417_19700 - cobyrinic acid a,c-diamide synthase, at 4,280,953 to 4,281,786
_19700
PS417_19705: PS417_19705 - flagellar biosynthesis regulator FlhF, at 4,281,893 to 4,283,209
_19705
PS417_19710: PS417_19710 - flagellar biosynthesis protein FlhA, at 4,283,222 to 4,285,351
_19710
Position (kb)
4281
4282
4283
4284 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4280.941 kb on - strand at 4280.956 kb on + strand at 4281.823 kb on - strand at 4281.864 kb on - strand at 4282.474 kb on - strand, within PS417_19705 at 4282.482 kb on - strand, within PS417_19705 at 4283.074 kb on - strand, within PS417_19705 at 4283.498 kb on + strand, within PS417_19710 at 4283.499 kb on - strand at 4283.552 kb on + strand, within PS417_19710 at 4283.552 kb on + strand at 4283.552 kb on + strand, within PS417_19710 at 4283.552 kb on + strand, within PS417_19710 at 4283.614 kb on + strand, within PS417_19710 at 4283.835 kb on + strand, within PS417_19710 at 4283.836 kb on - strand, within PS417_19710 at 4283.836 kb on - strand, within PS417_19710 at 4283.969 kb on - strand, within PS417_19710 at 4284.027 kb on + strand, within PS417_19710
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 4,280,941 - +3.3 4,280,956 + +1.1 4,281,823 - -4.2 4,281,864 - -2.1 4,282,474 - PS417_19705 0.44 -1.0 4,282,482 - PS417_19705 0.45 -0.5 4,283,074 - PS417_19705 0.90 -3.1 4,283,498 + PS417_19710 0.13 -0.9 4,283,499 - +0.7 4,283,552 + PS417_19710 0.15 -1.3 4,283,552 + +3.0 4,283,552 + PS417_19710 0.15 +0.0 4,283,552 + PS417_19710 0.15 -0.4 4,283,614 + PS417_19710 0.18 +0.4 4,283,835 + PS417_19710 0.29 +1.0 4,283,836 - PS417_19710 0.29 -0.2 4,283,836 - PS417_19710 0.29 -0.9 4,283,969 - PS417_19710 0.35 +0.7 4,284,027 + PS417_19710 0.38 +1.5
Or see this region's nucleotide sequence