Strain Fitness in Pseudomonas simiae WCS417 around PS417_19705

Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_19695 and PS417_19700 overlap by 4 nucleotidesPS417_19700 and PS417_19705 are separated by 106 nucleotidesPS417_19705 and PS417_19710 are separated by 12 nucleotides PS417_19695: PS417_19695 - flagellar biosynthesis sigma factor, at 4,280,216 to 4,280,956 _19695 PS417_19700: PS417_19700 - cobyrinic acid a,c-diamide synthase, at 4,280,953 to 4,281,786 _19700 PS417_19705: PS417_19705 - flagellar biosynthesis regulator FlhF, at 4,281,893 to 4,283,209 _19705 PS417_19710: PS417_19710 - flagellar biosynthesis protein FlhA, at 4,283,222 to 4,285,351 _19710 Position (kb) 4281 4282 4283 4284Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 4280.941 kb on - strandat 4280.956 kb on + strandat 4281.823 kb on - strandat 4281.864 kb on - strandat 4282.474 kb on - strand, within PS417_19705at 4282.482 kb on - strand, within PS417_19705at 4283.074 kb on - strand, within PS417_19705at 4283.498 kb on + strand, within PS417_19710at 4283.499 kb on - strandat 4283.552 kb on + strand, within PS417_19710at 4283.552 kb on + strandat 4283.552 kb on + strand, within PS417_19710at 4283.552 kb on + strand, within PS417_19710at 4283.614 kb on + strand, within PS417_19710at 4283.835 kb on + strand, within PS417_19710at 4283.836 kb on - strand, within PS417_19710at 4283.836 kb on - strand, within PS417_19710at 4283.969 kb on - strand, within PS417_19710at 4284.027 kb on + strand, within PS417_19710

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
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4,280,941 - +3.3
4,280,956 + +1.1
4,281,823 - -4.2
4,281,864 - -2.1
4,282,474 - PS417_19705 0.44 -1.0
4,282,482 - PS417_19705 0.45 -0.5
4,283,074 - PS417_19705 0.90 -3.1
4,283,498 + PS417_19710 0.13 -0.9
4,283,499 - +0.7
4,283,552 + PS417_19710 0.15 -1.3
4,283,552 + +3.0
4,283,552 + PS417_19710 0.15 +0.0
4,283,552 + PS417_19710 0.15 -0.4
4,283,614 + PS417_19710 0.18 +0.4
4,283,835 + PS417_19710 0.29 +1.0
4,283,836 - PS417_19710 0.29 -0.2
4,283,836 - PS417_19710 0.29 -0.9
4,283,969 - PS417_19710 0.35 +0.7
4,284,027 + PS417_19710 0.38 +1.5

Or see this region's nucleotide sequence