Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_16480 and PS417_16485 are separated by 10 nucleotides PS417_16485 and PS417_16490 are separated by 54 nucleotides PS417_16490 and PS417_16495 are separated by 496 nucleotides
PS417_16480: PS417_16480 - recombination factor protein RarA, at 3,599,374 to 3,600,696
_16480
PS417_16485: PS417_16485 - membrane protein, at 3,600,707 to 3,601,330
_16485
PS417_16490: PS417_16490 - cell division protein FtsK, at 3,601,385 to 3,603,793
_16490
PS417_16495: PS417_16495 - leucyl/phenylalanyl-tRNA--protein transferase, at 3,604,290 to 3,604,970
_16495
Position (kb)
3601
3602
3603
3604 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3600.623 kb on + strand at 3600.624 kb on - strand at 3600.708 kb on + strand at 3601.573 kb on - strand at 3602.171 kb on - strand, within PS417_16490 at 3602.876 kb on - strand, within PS417_16490 at 3603.847 kb on - strand at 3603.954 kb on - strand at 3604.003 kb on - strand at 3604.065 kb on - strand at 3604.172 kb on + strand at 3604.225 kb on - strand at 3604.225 kb on - strand at 3604.304 kb on - strand at 3604.403 kb on - strand, within PS417_16495 at 3604.545 kb on + strand, within PS417_16495 at 3604.609 kb on - strand, within PS417_16495 at 3604.638 kb on - strand, within PS417_16495 at 3604.725 kb on + strand, within PS417_16495 at 3604.725 kb on + strand, within PS417_16495
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 3,600,623 + +0.1 3,600,624 - -0.1 3,600,708 + -1.5 3,601,573 - +0.3 3,602,171 - PS417_16490 0.33 -0.3 3,602,876 - PS417_16490 0.62 -2.3 3,603,847 - +2.6 3,603,954 - -1.2 3,604,003 - -1.4 3,604,065 - +0.7 3,604,172 + +2.9 3,604,225 - -0.5 3,604,225 - -0.4 3,604,304 - -0.2 3,604,403 - PS417_16495 0.17 -1.5 3,604,545 + PS417_16495 0.37 +1.6 3,604,609 - PS417_16495 0.47 +1.6 3,604,638 - PS417_16495 0.51 +0.4 3,604,725 + PS417_16495 0.64 +0.1 3,604,725 + PS417_16495 0.64 +1.4
Or see this region's nucleotide sequence