Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_14635 and PS417_14640 are separated by 385 nucleotides PS417_14640 and PS417_14645 are separated by 182 nucleotides
PS417_14635: PS417_14635 - GDSL family lipase, at 3,195,892 to 3,196,788
_14635
PS417_14640: PS417_14640 - dihydrodipicolinate synthase, at 3,197,174 to 3,198,037
_14640
PS417_14645: PS417_14645 - hypothetical protein, at 3,198,220 to 3,200,418
_14645
Position (kb)
3197
3198
3199 Strain fitness (log2 ratio)
-1
0
1
2 at 3196.386 kb on - strand, within PS417_14635 at 3196.487 kb on + strand, within PS417_14635 at 3196.488 kb on - strand, within PS417_14635 at 3196.488 kb on - strand, within PS417_14635 at 3196.718 kb on + strand at 3196.719 kb on - strand at 3196.813 kb on + strand at 3196.880 kb on + strand at 3196.915 kb on + strand at 3197.191 kb on + strand at 3197.191 kb on + strand at 3197.192 kb on - strand at 3198.019 kb on - strand at 3198.019 kb on - strand at 3198.504 kb on - strand, within PS417_14645 at 3198.543 kb on - strand at 3198.753 kb on + strand, within PS417_14645 at 3198.855 kb on + strand, within PS417_14645 at 3198.857 kb on + strand, within PS417_14645 at 3198.858 kb on - strand, within PS417_14645 at 3198.858 kb on - strand, within PS417_14645
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 3,196,386 - PS417_14635 0.55 +0.6 3,196,487 + PS417_14635 0.66 +0.2 3,196,488 - PS417_14635 0.66 -1.1 3,196,488 - PS417_14635 0.66 +0.2 3,196,718 + -0.3 3,196,719 - -0.3 3,196,813 + +0.0 3,196,880 + -0.1 3,196,915 + +1.1 3,197,191 + -0.3 3,197,191 + +1.0 3,197,192 - +0.1 3,198,019 - -1.0 3,198,019 - +0.0 3,198,504 - PS417_14645 0.13 -0.9 3,198,543 - +2.6 3,198,753 + PS417_14645 0.24 +0.6 3,198,855 + PS417_14645 0.29 +0.3 3,198,857 + PS417_14645 0.29 -1.4 3,198,858 - PS417_14645 0.29 +0.6 3,198,858 - PS417_14645 0.29 +0.6
Or see this region's nucleotide sequence