Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12075 and PS417_12080 are separated by 263 nucleotides PS417_12080 and PS417_12085 overlap by 11 nucleotides PS417_12085 and PS417_12090 are separated by 64 nucleotides PS417_12090 and PS417_12095 are separated by 333 nucleotides
PS417_12075: PS417_12075 - DNA-binding protein, at 2,622,518 to 2,623,498
_12075
PS417_12080: PS417_12080 - membrane protein, at 2,623,762 to 2,625,324
_12080
PS417_12085: PS417_12085 - thioredoxin, at 2,625,314 to 2,625,649
_12085
PS417_12090: PS417_12090 - hypothetical protein, at 2,625,714 to 2,625,962
_12090
PS417_12095: PS417_12095 - ABC transporter substrate-binding protein, at 2,626,296 to 2,627,144
_12095
Position (kb)
2623
2624
2625
2626 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2622.834 kb on - strand, within PS417_12075 at 2622.981 kb on - strand, within PS417_12075 at 2622.981 kb on - strand, within PS417_12075 at 2622.981 kb on - strand, within PS417_12075 at 2622.997 kb on + strand, within PS417_12075 at 2622.997 kb on + strand, within PS417_12075 at 2622.998 kb on - strand, within PS417_12075 at 2622.998 kb on - strand, within PS417_12075 at 2622.998 kb on - strand, within PS417_12075 at 2623.046 kb on + strand, within PS417_12075 at 2623.046 kb on + strand, within PS417_12075 at 2623.047 kb on - strand, within PS417_12075 at 2623.183 kb on + strand, within PS417_12075 at 2623.184 kb on - strand, within PS417_12075 at 2623.262 kb on + strand, within PS417_12075 at 2623.429 kb on + strand at 2623.429 kb on + strand at 2623.562 kb on + strand at 2623.563 kb on - strand at 2623.641 kb on + strand at 2623.641 kb on + strand at 2623.641 kb on + strand at 2623.641 kb on + strand at 2623.641 kb on + strand at 2623.642 kb on - strand at 2623.953 kb on + strand, within PS417_12080 at 2624.767 kb on + strand at 2625.173 kb on - strand at 2625.342 kb on + strand at 2625.343 kb on - strand at 2625.415 kb on - strand, within PS417_12085 at 2625.501 kb on + strand, within PS417_12085 at 2625.501 kb on + strand, within PS417_12085 at 2625.565 kb on + strand at 2625.565 kb on + strand, within PS417_12085 at 2625.565 kb on + strand, within PS417_12085 at 2625.565 kb on + strand, within PS417_12085 at 2625.565 kb on + strand, within PS417_12085 at 2625.566 kb on - strand, within PS417_12085 at 2625.642 kb on + strand at 2625.643 kb on - strand at 2625.838 kb on + strand, within PS417_12090 at 2625.839 kb on - strand, within PS417_12090 at 2625.839 kb on - strand, within PS417_12090 at 2625.839 kb on - strand, within PS417_12090 at 2626.245 kb on + strand at 2626.319 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 2,622,834 - PS417_12075 0.32 +2.5 2,622,981 - PS417_12075 0.47 +2.6 2,622,981 - PS417_12075 0.47 +1.7 2,622,981 - PS417_12075 0.47 +2.6 2,622,997 + PS417_12075 0.49 +1.8 2,622,997 + PS417_12075 0.49 +2.4 2,622,998 - PS417_12075 0.49 +1.9 2,622,998 - PS417_12075 0.49 +3.2 2,622,998 - PS417_12075 0.49 +2.8 2,623,046 + PS417_12075 0.54 +2.3 2,623,046 + PS417_12075 0.54 +2.4 2,623,047 - PS417_12075 0.54 +2.5 2,623,183 + PS417_12075 0.68 +2.8 2,623,184 - PS417_12075 0.68 +2.1 2,623,262 + PS417_12075 0.76 +3.5 2,623,429 + +1.7 2,623,429 + +2.6 2,623,562 + -1.5 2,623,563 - -1.8 2,623,641 + -0.3 2,623,641 + -0.5 2,623,641 + +0.7 2,623,641 + +0.1 2,623,641 + -0.0 2,623,642 - +0.6 2,623,953 + PS417_12080 0.12 -0.4 2,624,767 + +0.6 2,625,173 - +0.2 2,625,342 + -0.1 2,625,343 - -1.4 2,625,415 - PS417_12085 0.30 -0.5 2,625,501 + PS417_12085 0.56 +0.1 2,625,501 + PS417_12085 0.56 -1.2 2,625,565 + -0.2 2,625,565 + PS417_12085 0.75 -0.0 2,625,565 + PS417_12085 0.75 -0.1 2,625,565 + PS417_12085 0.75 +0.4 2,625,565 + PS417_12085 0.75 +0.1 2,625,566 - PS417_12085 0.75 +0.4 2,625,642 + +2.2 2,625,643 - +1.3 2,625,838 + PS417_12090 0.50 +0.2 2,625,839 - PS417_12090 0.50 +0.8 2,625,839 - PS417_12090 0.50 +0.8 2,625,839 - PS417_12090 0.50 -1.1 2,626,245 + -1.4 2,626,319 + +0.8
Or see this region's nucleotide sequence