Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_09295 and PS417_09300 are separated by 258 nucleotides PS417_09300 and PS417_09305 are separated by 319 nucleotides PS417_09305 and PS417_09310 are separated by 94 nucleotides
PS417_09295: PS417_09295 - hypothetical protein, at 2,016,512 to 2,018,320
_09295
PS417_09300: PS417_09300 - peptidylprolyl isomerase, at 2,018,579 to 2,019,310
_09300
PS417_09305: PS417_09305 - hypothetical protein, at 2,019,630 to 2,020,088
_09305
PS417_09310: PS417_09310 - Cro/Cl family transcriptional regulator, at 2,020,183 to 2,020,524
_09310
Position (kb)
2018
2019
2020 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2017.725 kb on + strand, within PS417_09295 at 2017.729 kb on + strand, within PS417_09295 at 2017.729 kb on + strand, within PS417_09295 at 2017.776 kb on + strand, within PS417_09295 at 2017.777 kb on - strand, within PS417_09295 at 2017.777 kb on - strand, within PS417_09295 at 2017.777 kb on - strand, within PS417_09295 at 2017.777 kb on - strand, within PS417_09295 at 2017.779 kb on + strand, within PS417_09295 at 2017.779 kb on + strand, within PS417_09295 at 2017.779 kb on + strand, within PS417_09295 at 2017.780 kb on - strand, within PS417_09295 at 2017.900 kb on - strand, within PS417_09295 at 2017.900 kb on - strand, within PS417_09295 at 2018.059 kb on - strand, within PS417_09295 at 2018.160 kb on + strand at 2018.197 kb on - strand at 2018.221 kb on - strand at 2018.221 kb on - strand at 2018.247 kb on + strand at 2018.248 kb on - strand at 2018.248 kb on - strand at 2018.282 kb on + strand at 2018.283 kb on - strand at 2018.285 kb on + strand at 2018.286 kb on - strand at 2018.286 kb on - strand at 2018.364 kb on - strand at 2018.407 kb on - strand at 2018.460 kb on + strand at 2018.960 kb on + strand, within PS417_09300 at 2019.164 kb on + strand, within PS417_09300 at 2019.164 kb on + strand, within PS417_09300 at 2019.165 kb on - strand, within PS417_09300 at 2019.271 kb on + strand at 2019.309 kb on - strand at 2019.325 kb on + strand at 2019.325 kb on + strand at 2019.326 kb on - strand at 2019.326 kb on - strand at 2019.326 kb on - strand at 2019.326 kb on - strand at 2019.326 kb on - strand at 2019.375 kb on + strand at 2019.376 kb on - strand at 2019.420 kb on - strand at 2019.420 kb on - strand at 2019.431 kb on - strand at 2019.431 kb on - strand at 2019.431 kb on - strand at 2019.431 kb on - strand at 2019.433 kb on - strand at 2019.433 kb on - strand at 2019.433 kb on - strand at 2019.532 kb on + strand at 2019.801 kb on + strand, within PS417_09305 at 2019.802 kb on - strand, within PS417_09305 at 2019.867 kb on + strand, within PS417_09305 at 2019.868 kb on - strand, within PS417_09305 at 2020.094 kb on + strand at 2020.094 kb on + strand at 2020.094 kb on + strand at 2020.094 kb on + strand at 2020.095 kb on - strand at 2020.096 kb on + strand at 2020.149 kb on + strand at 2020.156 kb on + strand at 2020.156 kb on + strand at 2020.157 kb on - strand at 2020.166 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 2,017,725 + PS417_09295 0.67 -0.3 2,017,729 + PS417_09295 0.67 -0.1 2,017,729 + PS417_09295 0.67 +0.3 2,017,776 + PS417_09295 0.70 -0.0 2,017,777 - PS417_09295 0.70 +0.3 2,017,777 - PS417_09295 0.70 -0.2 2,017,777 - PS417_09295 0.70 -0.1 2,017,777 - PS417_09295 0.70 -0.3 2,017,779 + PS417_09295 0.70 -0.1 2,017,779 + PS417_09295 0.70 -1.3 2,017,779 + PS417_09295 0.70 -0.5 2,017,780 - PS417_09295 0.70 -0.2 2,017,900 - PS417_09295 0.77 +0.4 2,017,900 - PS417_09295 0.77 -0.1 2,018,059 - PS417_09295 0.86 +2.7 2,018,160 + +1.4 2,018,197 - +0.8 2,018,221 - +3.0 2,018,221 - -1.4 2,018,247 + -0.5 2,018,248 - +1.4 2,018,248 - -0.1 2,018,282 + +1.5 2,018,283 - +0.1 2,018,285 + -0.9 2,018,286 - -0.9 2,018,286 - -0.6 2,018,364 - +0.4 2,018,407 - -0.3 2,018,460 + -1.5 2,018,960 + PS417_09300 0.52 -0.3 2,019,164 + PS417_09300 0.80 -1.6 2,019,164 + PS417_09300 0.80 -3.1 2,019,165 - PS417_09300 0.80 -2.2 2,019,271 + -0.6 2,019,309 - -0.6 2,019,325 + +0.5 2,019,325 + +1.1 2,019,326 - +0.0 2,019,326 - -1.3 2,019,326 - -2.7 2,019,326 - -0.1 2,019,326 - +0.1 2,019,375 + -0.4 2,019,376 - -0.1 2,019,420 - -0.1 2,019,420 - +0.1 2,019,431 - -0.1 2,019,431 - +0.3 2,019,431 - -1.2 2,019,431 - +0.1 2,019,433 - +0.1 2,019,433 - -2.1 2,019,433 - -0.3 2,019,532 + -0.9 2,019,801 + PS417_09305 0.37 -0.1 2,019,802 - PS417_09305 0.37 +0.1 2,019,867 + PS417_09305 0.52 +0.7 2,019,868 - PS417_09305 0.52 -0.2 2,020,094 + -0.2 2,020,094 + +0.6 2,020,094 + -2.0 2,020,094 + -1.8 2,020,095 - -1.5 2,020,096 + +0.1 2,020,149 + +0.1 2,020,156 + -0.0 2,020,156 + +0.2 2,020,157 - -1.6 2,020,166 + +1.4
Or see this region's nucleotide sequence