Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_03365 and PS417_03370 are separated by 344 nucleotides PS417_03370 and PS417_03375 are separated by 225 nucleotides PS417_03375 and PS417_03380 are separated by 38 nucleotides
PS417_03365: PS417_03365 - transcriptional regulator, at 774,172 to 774,846
_03365
PS417_03370: PS417_03370 - porin, at 775,191 to 776,510
_03370
PS417_03375: PS417_03375 - hypothetical protein, at 776,736 to 777,068
_03375
PS417_03380: PS417_03380 - hypothetical protein, at 777,107 to 777,544
_03380
Position (kb) 775
776
777 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 774.557 kb on + strand, within PS417_03365 at 774.557 kb on + strand, within PS417_03365 at 774.557 kb on + strand, within PS417_03365 at 774.558 kb on - strand, within PS417_03365 at 774.767 kb on - strand, within PS417_03365 at 774.767 kb on - strand, within PS417_03365 at 774.774 kb on - strand, within PS417_03365 at 774.803 kb on + strand at 774.840 kb on + strand at 774.854 kb on + strand at 774.855 kb on - strand at 774.855 kb on - strand at 774.909 kb on - strand at 775.043 kb on + strand at 775.122 kb on + strand at 775.123 kb on - strand at 775.157 kb on + strand at 775.158 kb on - strand at 775.178 kb on + strand at 775.178 kb on + strand at 775.178 kb on + strand at 775.321 kb on + strand at 775.321 kb on + strand at 775.322 kb on - strand at 775.393 kb on + strand, within PS417_03370 at 775.393 kb on + strand, within PS417_03370 at 775.394 kb on - strand, within PS417_03370 at 775.414 kb on + strand, within PS417_03370 at 775.537 kb on + strand, within PS417_03370 at 775.537 kb on + strand, within PS417_03370 at 775.582 kb on + strand, within PS417_03370 at 775.583 kb on - strand, within PS417_03370 at 775.700 kb on + strand, within PS417_03370 at 775.700 kb on + strand, within PS417_03370 at 775.700 kb on + strand, within PS417_03370 at 775.700 kb on + strand, within PS417_03370 at 775.701 kb on - strand, within PS417_03370 at 775.720 kb on + strand, within PS417_03370 at 775.720 kb on + strand, within PS417_03370 at 775.721 kb on - strand, within PS417_03370 at 775.721 kb on - strand, within PS417_03370 at 775.723 kb on + strand, within PS417_03370 at 775.724 kb on - strand, within PS417_03370 at 775.724 kb on - strand, within PS417_03370 at 775.724 kb on - strand, within PS417_03370 at 775.786 kb on + strand, within PS417_03370 at 775.786 kb on + strand, within PS417_03370 at 775.903 kb on + strand, within PS417_03370 at 775.903 kb on + strand, within PS417_03370 at 776.076 kb on + strand, within PS417_03370 at 776.076 kb on + strand, within PS417_03370 at 776.140 kb on + strand, within PS417_03370 at 776.140 kb on + strand, within PS417_03370 at 776.141 kb on - strand, within PS417_03370 at 776.141 kb on - strand, within PS417_03370 at 776.141 kb on - strand, within PS417_03370 at 776.494 kb on + strand at 776.494 kb on + strand at 776.626 kb on - strand at 776.662 kb on + strand at 776.663 kb on - strand at 776.817 kb on - strand, within PS417_03375 at 776.817 kb on - strand, within PS417_03375 at 776.817 kb on - strand, within PS417_03375 at 776.840 kb on + strand, within PS417_03375 at 776.994 kb on + strand, within PS417_03375 at 776.995 kb on - strand, within PS417_03375 at 776.995 kb on - strand, within PS417_03375 at 777.020 kb on + strand, within PS417_03375 at 777.031 kb on - strand, within PS417_03375 at 777.252 kb on + strand, within PS417_03380 at 777.454 kb on - strand, within PS417_03380
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x remove 774,557 + PS417_03365 0.57 -1.0 774,557 + PS417_03365 0.57 -0.9 774,557 + PS417_03365 0.57 -0.4 774,558 - PS417_03365 0.57 +0.9 774,767 - PS417_03365 0.88 +0.4 774,767 - PS417_03365 0.88 +0.8 774,774 - PS417_03365 0.89 +0.3 774,803 + -0.3 774,840 + +1.2 774,854 + -0.2 774,855 - +0.8 774,855 - -0.3 774,909 - +0.2 775,043 + -0.4 775,122 + +0.7 775,123 - -0.2 775,157 + -0.2 775,158 - -2.4 775,178 + -0.1 775,178 + -0.1 775,178 + +1.8 775,321 + +0.4 775,321 + -0.7 775,322 - -2.0 775,393 + PS417_03370 0.15 +0.1 775,393 + PS417_03370 0.15 -0.3 775,394 - PS417_03370 0.15 -2.0 775,414 + PS417_03370 0.17 -0.2 775,537 + PS417_03370 0.26 +0.6 775,537 + PS417_03370 0.26 -0.5 775,582 + PS417_03370 0.30 +1.8 775,583 - PS417_03370 0.30 -1.7 775,700 + PS417_03370 0.39 -0.4 775,700 + PS417_03370 0.39 +0.2 775,700 + PS417_03370 0.39 +0.7 775,700 + PS417_03370 0.39 +0.0 775,701 - PS417_03370 0.39 -0.8 775,720 + PS417_03370 0.40 +2.8 775,720 + PS417_03370 0.40 -1.5 775,721 - PS417_03370 0.40 -0.6 775,721 - PS417_03370 0.40 -0.7 775,723 + PS417_03370 0.40 +0.2 775,724 - PS417_03370 0.40 +1.2 775,724 - PS417_03370 0.40 +2.6 775,724 - PS417_03370 0.40 -0.7 775,786 + PS417_03370 0.45 -1.6 775,786 + PS417_03370 0.45 -1.4 775,903 + PS417_03370 0.54 -0.7 775,903 + PS417_03370 0.54 -0.4 776,076 + PS417_03370 0.67 -0.0 776,076 + PS417_03370 0.67 -0.5 776,140 + PS417_03370 0.72 +0.6 776,140 + PS417_03370 0.72 -0.9 776,141 - PS417_03370 0.72 +1.6 776,141 - PS417_03370 0.72 -0.9 776,141 - PS417_03370 0.72 -0.5 776,494 + +0.2 776,494 + -0.6 776,626 - -0.7 776,662 + -0.1 776,663 - -0.8 776,817 - PS417_03375 0.24 -1.0 776,817 - PS417_03375 0.24 +0.5 776,817 - PS417_03375 0.24 +0.4 776,840 + PS417_03375 0.31 +0.1 776,994 + PS417_03375 0.77 -2.3 776,995 - PS417_03375 0.78 -0.7 776,995 - PS417_03375 0.78 -0.4 777,020 + PS417_03375 0.85 +0.2 777,031 - PS417_03375 0.89 +0.2 777,252 + PS417_03380 0.33 +0.3 777,454 - PS417_03380 0.79 -1.0
Or see this region's nucleotide sequence