Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00315 and PS417_00320 are separated by 6 nucleotides PS417_00320 and PS417_00325 are separated by 28 nucleotides PS417_00325 and PS417_00330 overlap by 35 nucleotides PS417_00330 and PS417_00335 are separated by 10 nucleotides
PS417_00315: PS417_00315 - MFS transporter, at 62,487 to 63,374
_00315
PS417_00320: PS417_00320 - hypothetical protein, at 63,381 to 63,584
_00320
PS417_00325: PS417_00325 - hypothetical protein, at 63,613 to 64,377
_00325
PS417_00330: PS417_00330 - hypothetical protein, at 64,343 to 64,927
_00330
PS417_00335: PS417_00335 - cytochrome B561, at 64,938 to 66,017
_00335
Position (kb) 64
65 Strain fitness (log2 ratio) -2
-1
0
1
2 at 63.364 kb on - strand at 63.364 kb on - strand at 63.364 kb on - strand at 63.624 kb on + strand at 63.624 kb on + strand at 63.625 kb on - strand at 63.625 kb on - strand at 63.625 kb on - strand at 63.625 kb on - strand at 63.625 kb on - strand at 63.627 kb on - strand at 63.638 kb on + strand at 63.638 kb on + strand at 63.639 kb on - strand at 63.760 kb on + strand, within PS417_00325 at 63.761 kb on - strand, within PS417_00325 at 63.761 kb on - strand, within PS417_00325 at 63.761 kb on - strand, within PS417_00325 at 63.761 kb on - strand, within PS417_00325 at 64.051 kb on + strand, within PS417_00325 at 64.052 kb on - strand, within PS417_00325 at 64.052 kb on - strand, within PS417_00325 at 64.174 kb on + strand, within PS417_00325 at 64.303 kb on + strand at 64.303 kb on + strand at 64.303 kb on + strand at 64.303 kb on + strand at 64.310 kb on - strand at 64.463 kb on + strand, within PS417_00330 at 64.463 kb on + strand at 64.463 kb on + strand, within PS417_00330 at 64.464 kb on - strand, within PS417_00330 at 64.499 kb on + strand, within PS417_00330 at 64.499 kb on + strand, within PS417_00330 at 64.542 kb on + strand, within PS417_00330 at 64.543 kb on - strand, within PS417_00330 at 64.543 kb on - strand, within PS417_00330 at 64.625 kb on + strand, within PS417_00330 at 64.625 kb on + strand, within PS417_00330 at 64.716 kb on - strand, within PS417_00330 at 64.853 kb on + strand, within PS417_00330 at 64.853 kb on + strand, within PS417_00330 at 65.010 kb on + strand at 65.012 kb on + strand at 65.013 kb on - strand at 65.013 kb on - strand at 65.013 kb on - strand at 65.061 kb on + strand, within PS417_00335 at 65.062 kb on - strand, within PS417_00335 at 65.172 kb on + strand, within PS417_00335 at 65.417 kb on - strand, within PS417_00335 at 65.603 kb on + strand, within PS417_00335 at 65.603 kb on + strand, within PS417_00335 at 65.603 kb on + strand, within PS417_00335 at 65.603 kb on + strand, within PS417_00335 at 65.604 kb on - strand, within PS417_00335 at 65.604 kb on - strand, within PS417_00335 at 65.625 kb on + strand, within PS417_00335 at 65.625 kb on + strand, within PS417_00335 at 65.626 kb on - strand, within PS417_00335 at 65.626 kb on - strand, within PS417_00335 at 65.626 kb on - strand, within PS417_00335 at 65.740 kb on + strand, within PS417_00335 at 65.741 kb on - strand, within PS417_00335 at 65.741 kb on - strand, within PS417_00335 at 65.745 kb on - strand, within PS417_00335 at 65.814 kb on + strand, within PS417_00335 at 65.815 kb on - strand, within PS417_00335 at 65.895 kb on + strand, within PS417_00335 at 65.896 kb on - strand, within PS417_00335 at 65.896 kb on - strand, within PS417_00335
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x remove 63,364 - -0.3 63,364 - -0.7 63,364 - -0.6 63,624 + +0.2 63,624 + -0.1 63,625 - -0.3 63,625 - -0.6 63,625 - +0.4 63,625 - -0.4 63,625 - -0.3 63,627 - -1.1 63,638 + -0.4 63,638 + -0.3 63,639 - -0.8 63,760 + PS417_00325 0.19 -0.9 63,761 - PS417_00325 0.19 -0.0 63,761 - PS417_00325 0.19 -0.7 63,761 - PS417_00325 0.19 -0.4 63,761 - PS417_00325 0.19 +0.2 64,051 + PS417_00325 0.57 +0.9 64,052 - PS417_00325 0.57 -1.1 64,052 - PS417_00325 0.57 +1.8 64,174 + PS417_00325 0.73 -2.3 64,303 + +0.6 64,303 + +0.5 64,303 + -0.0 64,303 + -0.2 64,310 - +0.4 64,463 + PS417_00330 0.21 -0.8 64,463 + -0.6 64,463 + PS417_00330 0.21 +1.2 64,464 - PS417_00330 0.21 -0.3 64,499 + PS417_00330 0.27 +1.2 64,499 + PS417_00330 0.27 -0.6 64,542 + PS417_00330 0.34 +0.1 64,543 - PS417_00330 0.34 +0.6 64,543 - PS417_00330 0.34 +0.2 64,625 + PS417_00330 0.48 -0.7 64,625 + PS417_00330 0.48 +0.1 64,716 - PS417_00330 0.64 -0.6 64,853 + PS417_00330 0.87 -0.8 64,853 + PS417_00330 0.87 -0.4 65,010 + +0.6 65,012 + -0.7 65,013 - +0.6 65,013 - +0.9 65,013 - +2.4 65,061 + PS417_00335 0.11 -0.8 65,062 - PS417_00335 0.11 -0.4 65,172 + PS417_00335 0.22 +0.2 65,417 - PS417_00335 0.44 +0.0 65,603 + PS417_00335 0.62 +0.2 65,603 + PS417_00335 0.62 -0.5 65,603 + PS417_00335 0.62 +1.6 65,603 + PS417_00335 0.62 -1.8 65,604 - PS417_00335 0.62 -0.8 65,604 - PS417_00335 0.62 +0.9 65,625 + PS417_00335 0.64 -0.1 65,625 + PS417_00335 0.64 -0.5 65,626 - PS417_00335 0.64 +0.1 65,626 - PS417_00335 0.64 -0.3 65,626 - PS417_00335 0.64 -0.1 65,740 + PS417_00335 0.74 +0.4 65,741 - PS417_00335 0.74 +0.5 65,741 - PS417_00335 0.74 -1.4 65,745 - PS417_00335 0.75 +0.9 65,814 + PS417_00335 0.81 -1.5 65,815 - PS417_00335 0.81 -1.0 65,895 + PS417_00335 0.89 +2.2 65,896 - PS417_00335 0.89 +1.6 65,896 - PS417_00335 0.89 -0.7
Or see this region's nucleotide sequence