Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02740 and PS417_02745 are separated by 136 nucleotides PS417_02745 and PS417_02750 are separated by 80 nucleotides PS417_02750 and PS417_02755 overlap by 4 nucleotides
PS417_02740: PS417_02740 - LysR family transcriptional regulator, at 624,631 to 625,530
_02740
PS417_02745: PS417_02745 - FMN-dependent NADH-azoreductase, at 625,667 to 626,308
_02745
PS417_02750: PS417_02750 - alpha/beta hydrolase, at 626,389 to 627,279
_02750
PS417_02755: PS417_02755 - membrane protein, at 627,276 to 628,145
_02755
Position (kb)
625
626
627 Strain fitness (log2 ratio)
-1
0
1
2 at 625.028 kb on + strand, within PS417_02740 at 625.028 kb on + strand, within PS417_02740 at 625.029 kb on - strand, within PS417_02740 at 625.663 kb on - strand at 626.186 kb on + strand, within PS417_02745 at 626.187 kb on - strand, within PS417_02745 at 626.187 kb on - strand at 626.247 kb on + strand at 626.247 kb on + strand at 626.248 kb on - strand at 626.248 kb on - strand at 626.261 kb on + strand at 626.261 kb on + strand at 626.262 kb on - strand at 626.307 kb on - strand at 626.347 kb on + strand at 626.348 kb on - strand at 626.358 kb on + strand at 626.358 kb on + strand at 626.359 kb on - strand at 626.359 kb on - strand at 626.359 kb on - strand at 626.361 kb on + strand at 626.361 kb on + strand at 626.361 kb on + strand at 626.362 kb on - strand at 626.556 kb on - strand, within PS417_02750 at 626.556 kb on - strand, within PS417_02750 at 626.756 kb on - strand, within PS417_02750 at 626.923 kb on + strand, within PS417_02750 at 626.990 kb on - strand, within PS417_02750 at 627.127 kb on + strand, within PS417_02750 at 627.128 kb on - strand, within PS417_02750 at 627.163 kb on + strand, within PS417_02750 at 627.164 kb on - strand, within PS417_02750
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x remove 625,028 + PS417_02740 0.44 +1.0 625,028 + PS417_02740 0.44 +0.3 625,029 - PS417_02740 0.44 +1.2 625,663 - -0.2 626,186 + PS417_02745 0.81 +1.4 626,187 - PS417_02745 0.81 +0.9 626,187 - +0.4 626,247 + +0.6 626,247 + +1.4 626,248 - +0.1 626,248 - +0.5 626,261 + -0.4 626,261 + +0.2 626,262 - +0.9 626,307 - +0.1 626,347 + -0.6 626,348 - +0.2 626,358 + -0.8 626,358 + +1.6 626,359 - +2.5 626,359 - +0.5 626,359 - -0.7 626,361 + -0.9 626,361 + -0.0 626,361 + +1.4 626,362 - +0.2 626,556 - PS417_02750 0.19 -0.3 626,556 - PS417_02750 0.19 -0.5 626,756 - PS417_02750 0.41 +0.3 626,923 + PS417_02750 0.60 +0.4 626,990 - PS417_02750 0.67 -0.0 627,127 + PS417_02750 0.83 -0.6 627,128 - PS417_02750 0.83 -0.7 627,163 + PS417_02750 0.87 +1.2 627,164 - PS417_02750 0.87 -0.3
Or see this region's nucleotide sequence