Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_26720 and PS417_26725 are separated by 57 nucleotides PS417_26725 and PS417_26730 are separated by 34 nucleotides PS417_26730 and PS417_26735 are separated by 9 nucleotides
PS417_26720: PS417_26720 - twitching motility protein PilT, at 5,774,718 to 5,775,752
_26720
PS417_26725: PS417_26725 - hypothetical protein, at 5,775,810 to 5,776,496
_26725
PS417_26730: PS417_26730 - pyrroline-5-carboxylate reductase, at 5,776,531 to 5,777,349
_26730
PS417_26735: PS417_26735 - membrane protein, at 5,777,359 to 5,777,949
_26735
Position (kb) 5775
5776
5777 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5775.261 kb on - strand, within PS417_26720 at 5775.411 kb on + strand, within PS417_26720 at 5775.411 kb on + strand, within PS417_26720 at 5775.412 kb on - strand, within PS417_26720 at 5775.412 kb on - strand, within PS417_26720 at 5775.461 kb on + strand, within PS417_26720 at 5775.603 kb on + strand, within PS417_26720 at 5775.603 kb on + strand, within PS417_26720 at 5775.604 kb on - strand, within PS417_26720 at 5775.604 kb on - strand, within PS417_26720 at 5775.736 kb on - strand at 5775.949 kb on - strand, within PS417_26725 at 5775.949 kb on - strand, within PS417_26725 at 5775.949 kb on - strand, within PS417_26725 at 5775.949 kb on - strand, within PS417_26725 at 5775.981 kb on + strand, within PS417_26725 at 5775.981 kb on + strand, within PS417_26725 at 5775.981 kb on + strand, within PS417_26725 at 5775.982 kb on - strand, within PS417_26725 at 5776.015 kb on + strand, within PS417_26725 at 5776.545 kb on + strand at 5776.733 kb on + strand, within PS417_26730 at 5777.062 kb on + strand, within PS417_26730 at 5777.347 kb on + strand at 5777.347 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x remove 5,775,261 - PS417_26720 0.52 +0.0 5,775,411 + PS417_26720 0.67 +3.2 5,775,411 + PS417_26720 0.67 +1.0 5,775,412 - PS417_26720 0.67 -0.7 5,775,412 - PS417_26720 0.67 +0.9 5,775,461 + PS417_26720 0.72 +0.3 5,775,603 + PS417_26720 0.86 +0.8 5,775,603 + PS417_26720 0.86 -0.2 5,775,604 - PS417_26720 0.86 -1.3 5,775,604 - PS417_26720 0.86 -0.4 5,775,736 - +0.9 5,775,949 - PS417_26725 0.20 +0.2 5,775,949 - PS417_26725 0.20 -2.5 5,775,949 - PS417_26725 0.20 -0.8 5,775,949 - PS417_26725 0.20 -0.1 5,775,981 + PS417_26725 0.25 -3.6 5,775,981 + PS417_26725 0.25 -0.3 5,775,981 + PS417_26725 0.25 -3.6 5,775,982 - PS417_26725 0.25 -2.1 5,776,015 + PS417_26725 0.30 -0.7 5,776,545 + -0.7 5,776,733 + PS417_26730 0.25 -2.3 5,777,062 + PS417_26730 0.65 -1.4 5,777,347 + +0.5 5,777,347 + -0.4
Or see this region's nucleotide sequence