Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_26275 and PS417_26280 are separated by 73 nucleotides PS417_26280 and PS417_26285 are separated by 142 nucleotides
PS417_26275: PS417_26275 - glycine/betaine ABC transporter substrate-binding protein, at 5,678,400 to 5,679,344
_26275
PS417_26280: PS417_26280 - NADPH:quinone reductase, at 5,679,418 to 5,680,305
_26280
PS417_26285: PS417_26285 - 3-hydroxybutyryl-CoA dehydrogenase, at 5,680,448 to 5,681,413
_26285
Position (kb)
5679
5680
5681 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5678.464 kb on + strand at 5678.527 kb on + strand, within PS417_26275 at 5678.769 kb on + strand, within PS417_26275 at 5678.769 kb on + strand, within PS417_26275 at 5678.770 kb on - strand, within PS417_26275 at 5678.770 kb on - strand, within PS417_26275 at 5679.342 kb on + strand at 5679.343 kb on - strand at 5679.396 kb on + strand at 5679.397 kb on - strand at 5679.841 kb on + strand, within PS417_26280 at 5679.841 kb on + strand, within PS417_26280 at 5679.842 kb on - strand, within PS417_26280 at 5679.842 kb on - strand, within PS417_26280 at 5679.850 kb on - strand, within PS417_26280 at 5680.358 kb on - strand at 5680.962 kb on - strand, within PS417_26285 at 5681.156 kb on + strand, within PS417_26285 at 5681.156 kb on + strand, within PS417_26285 at 5681.157 kb on - strand, within PS417_26285 at 5681.157 kb on - strand, within PS417_26285 at 5681.234 kb on + strand, within PS417_26285 at 5681.235 kb on - strand, within PS417_26285 at 5681.235 kb on - strand, within PS417_26285
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x remove 5,678,464 + -0.3 5,678,527 + PS417_26275 0.13 +0.3 5,678,769 + PS417_26275 0.39 -0.4 5,678,769 + PS417_26275 0.39 +0.9 5,678,770 - PS417_26275 0.39 +0.6 5,678,770 - PS417_26275 0.39 +1.9 5,679,342 + +0.1 5,679,343 - -1.5 5,679,396 + -0.4 5,679,397 - -0.1 5,679,841 + PS417_26280 0.48 -1.4 5,679,841 + PS417_26280 0.48 -1.4 5,679,842 - PS417_26280 0.48 -1.2 5,679,842 - PS417_26280 0.48 -0.0 5,679,850 - PS417_26280 0.49 -1.8 5,680,358 - +0.6 5,680,962 - PS417_26285 0.53 -0.1 5,681,156 + PS417_26285 0.73 -0.1 5,681,156 + PS417_26285 0.73 +0.6 5,681,157 - PS417_26285 0.73 +0.6 5,681,157 - PS417_26285 0.73 -0.6 5,681,234 + PS417_26285 0.81 -0.3 5,681,235 - PS417_26285 0.81 -0.4 5,681,235 - PS417_26285 0.81 +1.0
Or see this region's nucleotide sequence