Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02400 and PS417_02405 are separated by 173 nucleotides PS417_02405 and PS417_02410 are separated by 175 nucleotides
PS417_02400: PS417_02400 - DNA topoisomerase IV subunit A, at 548,450 to 550,714
_02400
PS417_02405: PS417_02405 - hypothetical protein, at 550,888 to 551,601
_02405
PS417_02410: PS417_02410 - histidine kinase, at 551,777 to 553,279
_02410
Position (kb)
550
551
552 Strain fitness (log2 ratio)
-1
0
1 at 550.797 kb on + strand at 550.833 kb on + strand at 550.844 kb on + strand at 550.850 kb on + strand at 550.850 kb on + strand at 550.850 kb on + strand at 550.850 kb on + strand at 550.851 kb on - strand at 550.978 kb on + strand, within PS417_02405 at 550.979 kb on - strand, within PS417_02405 at 551.236 kb on - strand, within PS417_02405 at 551.266 kb on - strand, within PS417_02405 at 551.343 kb on - strand, within PS417_02405 at 551.712 kb on + strand at 551.712 kb on + strand at 551.713 kb on - strand at 551.730 kb on + strand at 551.763 kb on + strand at 551.763 kb on + strand at 551.764 kb on - strand at 551.764 kb on - strand at 551.770 kb on + strand at 551.770 kb on + strand at 551.771 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x remove 550,797 + -0.6 550,833 + -0.6 550,844 + -0.2 550,850 + -1.0 550,850 + -1.5 550,850 + +1.1 550,850 + -0.1 550,851 - -0.3 550,978 + PS417_02405 0.13 -0.5 550,979 - PS417_02405 0.13 -0.1 551,236 - PS417_02405 0.49 +0.1 551,266 - PS417_02405 0.53 -0.1 551,343 - PS417_02405 0.64 +0.0 551,712 + +0.5 551,712 + -1.3 551,713 - +1.3 551,730 + -1.1 551,763 + -0.2 551,763 + -0.3 551,764 - -0.3 551,764 - +0.1 551,770 + +0.6 551,770 + +0.4 551,771 - +0.7
Or see this region's nucleotide sequence