Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_19560 and PS417_19565 are separated by 94 nucleotides PS417_19565 and PS417_19570 are separated by 153 nucleotides PS417_19570 and PS417_19575 are separated by 13 nucleotides PS417_19575 and PS417_19580 are separated by 3 nucleotides
PS417_19560: PS417_19560 - amino acid ABC transporter substrate-binding protein, at 4,254,085 to 4,254,873
_19560
PS417_19565: PS417_19565 - TetR family transcriptional regulator, at 4,254,968 to 4,255,591
_19565
PS417_19570: PS417_19570 - terpene utilization protein AtuA, at 4,255,745 to 4,257,523
_19570
PS417_19575: PS417_19575 - 2,4-dienoyl-CoA reductase, at 4,257,537 to 4,258,409
_19575
PS417_19580: PS417_19580 - acetyl-CoA carboxylase, at 4,258,413 to 4,260,029
_19580
Position (kb)
4255
4256
4257
4258 Strain fitness (log2 ratio)
-1
0
1
2
3 at 4254.755 kb on + strand, within PS417_19560 at 4254.954 kb on + strand at 4255.095 kb on - strand at 4255.110 kb on + strand, within PS417_19565 at 4255.136 kb on + strand, within PS417_19565 at 4255.137 kb on - strand, within PS417_19565 at 4255.159 kb on - strand, within PS417_19565 at 4255.347 kb on + strand, within PS417_19565 at 4255.348 kb on - strand, within PS417_19565 at 4255.633 kb on + strand at 4256.181 kb on - strand, within PS417_19570 at 4256.309 kb on + strand, within PS417_19570 at 4256.345 kb on - strand, within PS417_19570 at 4256.345 kb on - strand, within PS417_19570 at 4256.345 kb on - strand, within PS417_19570 at 4256.368 kb on + strand, within PS417_19570 at 4256.433 kb on + strand, within PS417_19570 at 4256.434 kb on - strand, within PS417_19570 at 4256.549 kb on + strand, within PS417_19570 at 4256.657 kb on + strand, within PS417_19570 at 4256.658 kb on - strand, within PS417_19570 at 4256.675 kb on + strand, within PS417_19570 at 4256.808 kb on - strand, within PS417_19570 at 4257.267 kb on - strand, within PS417_19570 at 4257.267 kb on - strand, within PS417_19570 at 4257.362 kb on + strand at 4257.362 kb on + strand at 4257.362 kb on + strand at 4257.363 kb on - strand at 4258.143 kb on + strand, within PS417_19575 at 4258.143 kb on + strand, within PS417_19575 at 4258.377 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x remove 4,254,755 + PS417_19560 0.85 +1.7 4,254,954 + -0.2 4,255,095 - -0.7 4,255,110 + PS417_19565 0.23 +0.1 4,255,136 + PS417_19565 0.27 +0.3 4,255,137 - PS417_19565 0.27 -0.6 4,255,159 - PS417_19565 0.31 +0.5 4,255,347 + PS417_19565 0.61 -0.9 4,255,348 - PS417_19565 0.61 -0.8 4,255,633 + -0.0 4,256,181 - PS417_19570 0.25 +0.0 4,256,309 + PS417_19570 0.32 +0.5 4,256,345 - PS417_19570 0.34 -1.5 4,256,345 - PS417_19570 0.34 +1.2 4,256,345 - PS417_19570 0.34 -0.2 4,256,368 + PS417_19570 0.35 -0.4 4,256,433 + PS417_19570 0.39 +0.4 4,256,434 - PS417_19570 0.39 -0.3 4,256,549 + PS417_19570 0.45 -0.4 4,256,657 + PS417_19570 0.51 -1.1 4,256,658 - PS417_19570 0.51 -0.1 4,256,675 + PS417_19570 0.52 +0.0 4,256,808 - PS417_19570 0.60 -0.0 4,257,267 - PS417_19570 0.86 +1.9 4,257,267 - PS417_19570 0.86 -0.1 4,257,362 + +0.0 4,257,362 + +0.1 4,257,362 + +0.3 4,257,363 - +2.9 4,258,143 + PS417_19575 0.69 +0.4 4,258,143 + PS417_19575 0.69 -0.3 4,258,377 + +0.9
Or see this region's nucleotide sequence