Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_14380 and PS417_14385 are separated by 348 nucleotides PS417_14385 and PS417_14390 are separated by 212 nucleotides PS417_14390 and PS417_14395 are separated by 236 nucleotides PS417_14395 and PS417_14400 are separated by 6 nucleotides PS417_14400 and PS417_14405 are separated by 111 nucleotides
PS417_14380: PS417_14380 - hypothetical protein, at 3,142,953 to 3,145,391
_14380
PS417_14385: PS417_14385 - tRNA-Cys, at 3,145,740 to 3,145,813
_14385
PS417_14390: PS417_14390 - acyl-homoserine lactone acylase subunit beta, at 3,146,026 to 3,148,311
_14390
PS417_14395: PS417_14395 - fe2+ zn2+ uptake regulation protein, at 3,148,548 to 3,148,928
_14395
PS417_14400: PS417_14400 - hypothetical protein, at 3,148,935 to 3,149,123
_14400
PS417_14405: PS417_14405 - ABC transporter permease, at 3,149,235 to 3,149,996
_14405
Position (kb)
3146
3147
3148
3149 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3145.083 kb on + strand, within PS417_14380 at 3145.277 kb on - strand at 3145.470 kb on + strand at 3145.470 kb on + strand at 3145.470 kb on + strand at 3145.470 kb on + strand at 3145.470 kb on + strand at 3145.470 kb on + strand at 3145.471 kb on - strand at 3145.471 kb on - strand at 3145.471 kb on - strand at 3145.471 kb on - strand at 3145.471 kb on - strand at 3145.472 kb on + strand at 3145.472 kb on + strand at 3145.493 kb on + strand at 3145.494 kb on - strand at 3145.567 kb on + strand at 3146.288 kb on + strand, within PS417_14390 at 3146.289 kb on - strand, within PS417_14390 at 3146.688 kb on - strand, within PS417_14390 at 3146.688 kb on - strand, within PS417_14390 at 3146.688 kb on - strand, within PS417_14390 at 3146.688 kb on - strand, within PS417_14390 at 3147.069 kb on - strand, within PS417_14390 at 3147.116 kb on - strand, within PS417_14390 at 3147.295 kb on - strand, within PS417_14390 at 3147.295 kb on - strand, within PS417_14390 at 3147.295 kb on - strand, within PS417_14390 at 3147.295 kb on - strand, within PS417_14390 at 3147.295 kb on - strand, within PS417_14390 at 3147.296 kb on + strand, within PS417_14390 at 3147.296 kb on + strand, within PS417_14390 at 3147.297 kb on - strand, within PS417_14390 at 3147.393 kb on + strand, within PS417_14390 at 3147.393 kb on + strand, within PS417_14390 at 3147.393 kb on + strand, within PS417_14390 at 3147.394 kb on - strand, within PS417_14390 at 3147.394 kb on - strand, within PS417_14390 at 3147.395 kb on + strand, within PS417_14390 at 3147.395 kb on + strand, within PS417_14390 at 3147.395 kb on + strand, within PS417_14390 at 3147.396 kb on - strand, within PS417_14390 at 3147.396 kb on - strand, within PS417_14390 at 3147.396 kb on - strand, within PS417_14390 at 3147.733 kb on + strand, within PS417_14390 at 3147.808 kb on + strand, within PS417_14390 at 3148.279 kb on - strand at 3148.353 kb on + strand at 3148.353 kb on + strand at 3148.353 kb on + strand at 3148.354 kb on - strand at 3148.364 kb on + strand at 3148.364 kb on + strand at 3148.364 kb on + strand at 3148.365 kb on - strand at 3148.407 kb on + strand at 3148.407 kb on + strand at 3148.493 kb on + strand at 3148.493 kb on + strand at 3148.493 kb on + strand at 3148.494 kb on - strand at 3148.551 kb on + strand at 3148.551 kb on + strand at 3148.551 kb on + strand at 3148.551 kb on + strand at 3148.552 kb on - strand at 3148.552 kb on - strand at 3148.552 kb on - strand at 3148.793 kb on - strand, within PS417_14395 at 3149.047 kb on + strand, within PS417_14400 at 3149.074 kb on - strand, within PS417_14400
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x remove 3,145,083 + PS417_14380 0.87 +0.2 3,145,277 - -0.2 3,145,470 + +0.2 3,145,470 + +0.5 3,145,470 + -0.2 3,145,470 + -0.6 3,145,470 + +1.1 3,145,470 + -0.5 3,145,471 - -0.1 3,145,471 - -2.0 3,145,471 - +0.8 3,145,471 - +0.1 3,145,471 - -0.2 3,145,472 + -0.0 3,145,472 + +0.3 3,145,493 + -0.0 3,145,494 - -0.6 3,145,567 + +0.8 3,146,288 + PS417_14390 0.11 +0.9 3,146,289 - PS417_14390 0.12 +1.0 3,146,688 - PS417_14390 0.29 +0.8 3,146,688 - PS417_14390 0.29 +1.7 3,146,688 - PS417_14390 0.29 +1.1 3,146,688 - PS417_14390 0.29 +1.8 3,147,069 - PS417_14390 0.46 +1.2 3,147,116 - PS417_14390 0.48 +0.9 3,147,295 - PS417_14390 0.56 +1.4 3,147,295 - PS417_14390 0.56 +1.2 3,147,295 - PS417_14390 0.56 +0.2 3,147,295 - PS417_14390 0.56 +0.7 3,147,295 - PS417_14390 0.56 +0.8 3,147,296 + PS417_14390 0.56 +0.6 3,147,296 + PS417_14390 0.56 +2.8 3,147,297 - PS417_14390 0.56 +1.3 3,147,393 + PS417_14390 0.60 +0.5 3,147,393 + PS417_14390 0.60 +0.4 3,147,393 + PS417_14390 0.60 +1.3 3,147,394 - PS417_14390 0.60 +0.8 3,147,394 - PS417_14390 0.60 +1.7 3,147,395 + PS417_14390 0.60 +1.6 3,147,395 + PS417_14390 0.60 +1.4 3,147,395 + PS417_14390 0.60 +1.2 3,147,396 - PS417_14390 0.60 +0.6 3,147,396 - PS417_14390 0.60 +1.4 3,147,396 - PS417_14390 0.60 -0.9 3,147,733 + PS417_14390 0.75 +2.4 3,147,808 + PS417_14390 0.78 +0.7 3,148,279 - +1.7 3,148,353 + +2.3 3,148,353 + +2.1 3,148,353 + +1.0 3,148,354 - -0.0 3,148,364 + +1.0 3,148,364 + +0.1 3,148,364 + -0.8 3,148,365 - +0.4 3,148,407 + -2.0 3,148,407 + -1.3 3,148,493 + -0.9 3,148,493 + -0.9 3,148,493 + -0.9 3,148,494 - -2.0 3,148,551 + -0.4 3,148,551 + -0.6 3,148,551 + -0.3 3,148,551 + -1.3 3,148,552 - -0.0 3,148,552 - -0.8 3,148,552 - +0.4 3,148,793 - PS417_14395 0.64 -0.2 3,149,047 + PS417_14400 0.59 +0.1 3,149,074 - PS417_14400 0.74 -0.4
Or see this region's nucleotide sequence