Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12205 and PS417_12210 overlap by 4 nucleotides PS417_12210 and PS417_12215 are separated by 211 nucleotides
PS417_12205: PS417_12205 - hypothetical protein, at 2,651,530 to 2,653,362
_12205
PS417_12210: PS417_12210 - ABC transporter substrate-binding protein, at 2,653,359 to 2,655,188
_12210
PS417_12215: PS417_12215 - lytic transglycosylase, at 2,655,400 to 2,656,815
_12215
Position (kb)
2653
2654
2655
2656 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2652.509 kb on + strand, within PS417_12205 at 2652.603 kb on - strand, within PS417_12205 at 2652.629 kb on + strand, within PS417_12205 at 2652.629 kb on + strand, within PS417_12205 at 2652.665 kb on + strand, within PS417_12205 at 2652.722 kb on + strand, within PS417_12205 at 2652.723 kb on - strand, within PS417_12205 at 2653.157 kb on + strand, within PS417_12205 at 2653.158 kb on - strand, within PS417_12205 at 2653.355 kb on + strand at 2653.355 kb on + strand at 2653.356 kb on - strand at 2653.356 kb on - strand at 2653.410 kb on - strand at 2653.463 kb on - strand at 2653.610 kb on - strand, within PS417_12210 at 2653.652 kb on - strand, within PS417_12210 at 2653.652 kb on - strand, within PS417_12210 at 2653.738 kb on + strand, within PS417_12210 at 2653.843 kb on - strand at 2653.992 kb on - strand, within PS417_12210 at 2654.090 kb on - strand, within PS417_12210 at 2654.108 kb on - strand, within PS417_12210 at 2654.329 kb on + strand, within PS417_12210 at 2654.330 kb on - strand, within PS417_12210 at 2654.330 kb on - strand, within PS417_12210 at 2654.330 kb on - strand, within PS417_12210 at 2654.330 kb on - strand, within PS417_12210 at 2654.330 kb on - strand, within PS417_12210 at 2654.407 kb on + strand, within PS417_12210 at 2654.408 kb on - strand, within PS417_12210 at 2654.408 kb on - strand, within PS417_12210 at 2654.408 kb on - strand, within PS417_12210 at 2654.408 kb on - strand, within PS417_12210 at 2654.414 kb on - strand, within PS417_12210 at 2654.936 kb on - strand, within PS417_12210 at 2655.108 kb on - strand at 2655.108 kb on - strand at 2655.162 kb on + strand at 2655.162 kb on + strand at 2655.163 kb on - strand at 2655.163 kb on - strand at 2655.163 kb on - strand at 2655.165 kb on + strand at 2655.165 kb on + strand at 2655.169 kb on - strand at 2655.169 kb on - strand at 2655.401 kb on + strand at 2655.401 kb on + strand at 2655.402 kb on - strand at 2655.513 kb on - strand at 2655.539 kb on + strand at 2655.540 kb on - strand at 2655.540 kb on - strand at 2655.716 kb on + strand, within PS417_12215 at 2655.716 kb on + strand, within PS417_12215 at 2655.717 kb on - strand, within PS417_12215 at 2655.726 kb on + strand, within PS417_12215 at 2656.028 kb on + strand, within PS417_12215 at 2656.080 kb on - strand, within PS417_12215 at 2656.185 kb on - strand, within PS417_12215
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x remove 2,652,509 + PS417_12205 0.53 +0.8 2,652,603 - PS417_12205 0.59 -1.0 2,652,629 + PS417_12205 0.60 -0.9 2,652,629 + PS417_12205 0.60 -2.8 2,652,665 + PS417_12205 0.62 +1.5 2,652,722 + PS417_12205 0.65 -1.2 2,652,723 - PS417_12205 0.65 -0.4 2,653,157 + PS417_12205 0.89 -0.8 2,653,158 - PS417_12205 0.89 -1.8 2,653,355 + -0.2 2,653,355 + -0.4 2,653,356 - -0.2 2,653,356 - -1.6 2,653,410 - -0.3 2,653,463 - -0.6 2,653,610 - PS417_12210 0.14 -0.5 2,653,652 - PS417_12210 0.16 +0.4 2,653,652 - PS417_12210 0.16 -0.6 2,653,738 + PS417_12210 0.21 -1.9 2,653,843 - -0.2 2,653,992 - PS417_12210 0.35 -0.7 2,654,090 - PS417_12210 0.40 -2.0 2,654,108 - PS417_12210 0.41 -0.7 2,654,329 + PS417_12210 0.53 +0.3 2,654,330 - PS417_12210 0.53 -1.2 2,654,330 - PS417_12210 0.53 -1.2 2,654,330 - PS417_12210 0.53 -2.5 2,654,330 - PS417_12210 0.53 +0.8 2,654,330 - PS417_12210 0.53 -0.9 2,654,407 + PS417_12210 0.57 -1.5 2,654,408 - PS417_12210 0.57 -0.8 2,654,408 - PS417_12210 0.57 +0.0 2,654,408 - PS417_12210 0.57 +0.0 2,654,408 - PS417_12210 0.57 -0.4 2,654,414 - PS417_12210 0.58 -0.2 2,654,936 - PS417_12210 0.86 -0.6 2,655,108 - -0.7 2,655,108 - -1.0 2,655,162 + -2.2 2,655,162 + -0.9 2,655,163 - -0.9 2,655,163 - +0.6 2,655,163 - -1.1 2,655,165 + -0.5 2,655,165 + -0.3 2,655,169 - +1.1 2,655,169 - -0.4 2,655,401 + -0.9 2,655,401 + +0.1 2,655,402 - -0.5 2,655,513 - +0.4 2,655,539 + -0.1 2,655,540 - +0.2 2,655,540 - +0.2 2,655,716 + PS417_12215 0.22 -1.8 2,655,716 + PS417_12215 0.22 -0.7 2,655,717 - PS417_12215 0.22 -0.4 2,655,726 + PS417_12215 0.23 -1.0 2,656,028 + PS417_12215 0.44 -0.9 2,656,080 - PS417_12215 0.48 -0.7 2,656,185 - PS417_12215 0.55 +0.4
Or see this region's nucleotide sequence