Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11255 and PS417_11260 overlap by 4 nucleotides PS417_11260 and PS417_11265 are separated by 93 nucleotides PS417_11265 and PS417_11270 overlap by 14 nucleotides PS417_11270 and PS417_11275 are separated by 148 nucleotides PS417_11275 and PS417_11280 are separated by 8 nucleotides
PS417_11255: PS417_11255 - general secretion pathway protein GspJ, at 2,411,841 to 2,412,452
_11255
PS417_11260: PS417_11260 - general secretion pathway protein GspH, at 2,412,449 to 2,412,901
_11260
PS417_11265: PS417_11265 - general secretion pathway protein GspC, at 2,412,995 to 2,413,426
_11265
PS417_11270: PS417_11270 - general secretion pathway protein GspI, at 2,413,413 to 2,413,778
_11270
PS417_11275: PS417_11275 - general secretion pathway protein GspG, at 2,413,927 to 2,414,376
_11275
PS417_11280: PS417_11280 - HxcX atypical pseudopilin, at 2,414,385 to 2,415,254
_11280
Position (kb)
2412
2413
2414 Strain fitness (log2 ratio)
-1
0
1 at 2412.420 kb on + strand at 2412.421 kb on - strand at 2412.861 kb on - strand at 2413.748 kb on + strand at 2413.774 kb on + strand at 2413.775 kb on - strand at 2413.863 kb on + strand at 2414.129 kb on - strand, within PS417_11275 at 2414.269 kb on + strand, within PS417_11275 at 2414.269 kb on + strand at 2414.345 kb on + strand at 2414.345 kb on + strand at 2414.375 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x remove 2,412,420 + +0.5 2,412,421 - -0.3 2,412,861 - +0.8 2,413,748 + -1.1 2,413,774 + +0.1 2,413,775 - -0.6 2,413,863 + -0.0 2,414,129 - PS417_11275 0.45 -1.5 2,414,269 + PS417_11275 0.76 -0.5 2,414,269 + +0.8 2,414,345 + -0.1 2,414,345 + -0.1 2,414,375 - +0.3
Or see this region's nucleotide sequence