Strain Fitness in Pseudomonas simiae WCS417 around PS417_11265

Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntPS417_11255 and PS417_11260 overlap by 4 nucleotidesPS417_11260 and PS417_11265 are separated by 93 nucleotidesPS417_11265 and PS417_11270 overlap by 14 nucleotidesPS417_11270 and PS417_11275 are separated by 148 nucleotidesPS417_11275 and PS417_11280 are separated by 8 nucleotides PS417_11255: PS417_11255 - general secretion pathway protein GspJ, at 2,411,841 to 2,412,452 _11255 PS417_11260: PS417_11260 - general secretion pathway protein GspH, at 2,412,449 to 2,412,901 _11260 PS417_11265: PS417_11265 - general secretion pathway protein GspC, at 2,412,995 to 2,413,426 _11265 PS417_11270: PS417_11270 - general secretion pathway protein GspI, at 2,413,413 to 2,413,778 _11270 PS417_11275: PS417_11275 - general secretion pathway protein GspG, at 2,413,927 to 2,414,376 _11275 PS417_11280: PS417_11280 - HxcX atypical pseudopilin, at 2,414,385 to 2,415,254 _11280 Position (kb) 2412 2413 2414Strain fitness (log2 ratio) -1 0 1at 2412.420 kb on + strandat 2412.421 kb on - strandat 2412.861 kb on - strandat 2413.748 kb on + strandat 2413.774 kb on + strandat 2413.775 kb on - strandat 2413.863 kb on + strandat 2414.129 kb on - strand, within PS417_11275at 2414.269 kb on + strand, within PS417_11275at 2414.269 kb on + strandat 2414.345 kb on + strandat 2414.345 kb on + strandat 2414.375 kb on - strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
remove
2,412,420 + +0.5
2,412,421 - -0.3
2,412,861 - +0.8
2,413,748 + -1.1
2,413,774 + +0.1
2,413,775 - -0.6
2,413,863 + -0.0
2,414,129 - PS417_11275 0.45 -1.5
2,414,269 + PS417_11275 0.76 -0.5
2,414,269 + +0.8
2,414,345 + -0.1
2,414,345 + -0.1
2,414,375 - +0.3

Or see this region's nucleotide sequence