Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11175 and PS417_11180 are separated by 173 nucleotides PS417_11180 and PS417_11185 overlap by 4 nucleotides PS417_11185 and PS417_11190 are separated by 79 nucleotides
PS417_11175: PS417_11175 - AraC family transcriptional regulator, at 2,397,187 to 2,398,125
_11175
PS417_11180: PS417_11180 - TetR family transcriptional regulator, at 2,398,299 to 2,398,928
_11180
PS417_11185: PS417_11185 - dimethylallyltransferase, at 2,398,925 to 2,399,602
_11185
PS417_11190: PS417_11190 - oxidoreductase, at 2,399,682 to 2,400,806
_11190
Position (kb)
2398
2399 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2397.502 kb on + strand, within PS417_11175 at 2397.502 kb on + strand, within PS417_11175 at 2398.281 kb on - strand at 2398.397 kb on - strand, within PS417_11180 at 2398.432 kb on - strand, within PS417_11180 at 2398.437 kb on + strand, within PS417_11180 at 2398.437 kb on + strand, within PS417_11180 at 2398.438 kb on - strand, within PS417_11180 at 2398.495 kb on - strand, within PS417_11180 at 2398.908 kb on + strand at 2398.933 kb on - strand at 2398.936 kb on - strand at 2398.941 kb on + strand at 2398.942 kb on - strand at 2399.016 kb on - strand, within PS417_11185 at 2399.219 kb on + strand, within PS417_11185 at 2399.439 kb on + strand, within PS417_11185 at 2399.440 kb on - strand, within PS417_11185 at 2399.605 kb on + strand at 2399.605 kb on + strand at 2399.606 kb on - strand at 2399.637 kb on + strand at 2399.726 kb on + strand at 2399.741 kb on + strand at 2399.741 kb on + strand at 2399.814 kb on + strand, within PS417_11190 at 2399.887 kb on - strand, within PS417_11190 at 2399.908 kb on - strand, within PS417_11190
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x remove 2,397,502 + PS417_11175 0.34 -1.2 2,397,502 + PS417_11175 0.34 -2.8 2,398,281 - -1.3 2,398,397 - PS417_11180 0.16 -1.8 2,398,432 - PS417_11180 0.21 -1.5 2,398,437 + PS417_11180 0.22 -0.7 2,398,437 + PS417_11180 0.22 +0.8 2,398,438 - PS417_11180 0.22 -0.2 2,398,495 - PS417_11180 0.31 -1.2 2,398,908 + -1.2 2,398,933 - -0.2 2,398,936 - +0.2 2,398,941 + -0.3 2,398,942 - -0.5 2,399,016 - PS417_11185 0.13 -2.2 2,399,219 + PS417_11185 0.43 -0.3 2,399,439 + PS417_11185 0.76 +2.4 2,399,440 - PS417_11185 0.76 -0.4 2,399,605 + -0.4 2,399,605 + -0.5 2,399,606 - -0.6 2,399,637 + -2.0 2,399,726 + +0.8 2,399,741 + -0.1 2,399,741 + -0.3 2,399,814 + PS417_11190 0.12 +0.5 2,399,887 - PS417_11190 0.18 -0.0 2,399,908 - PS417_11190 0.20 -0.2
Or see this region's nucleotide sequence