Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10900 and PS417_10905 are separated by 47 nucleotides PS417_10905 and PS417_10910 overlap by 1 nucleotides PS417_10910 and PS417_10915 overlap by 4 nucleotides
PS417_10900: PS417_10900 - ABC transporter substrate-binding protein, at 2,331,362 to 2,332,138
_10900
PS417_10905: PS417_10905 - hypothetical protein, at 2,332,186 to 2,332,812
_10905
PS417_10910: PS417_10910 - FAD-dependent oxidoreductase, at 2,332,812 to 2,333,945
_10910
PS417_10915: PS417_10915 - ornithine cyclodeaminase, at 2,333,942 to 2,334,886
_10915
Position (kb)
2332
2333
2334 Strain fitness (log2 ratio)
-1
0
1
2 at 2331.863 kb on + strand, within PS417_10900 at 2331.863 kb on + strand at 2331.864 kb on - strand, within PS417_10900 at 2332.127 kb on + strand at 2332.149 kb on - strand at 2332.149 kb on - strand at 2332.179 kb on - strand at 2332.179 kb on - strand at 2332.253 kb on + strand, within PS417_10905 at 2332.253 kb on + strand, within PS417_10905 at 2332.254 kb on - strand, within PS417_10905 at 2332.492 kb on - strand, within PS417_10905 at 2332.548 kb on + strand, within PS417_10905 at 2332.606 kb on + strand, within PS417_10905 at 2332.606 kb on + strand, within PS417_10905 at 2332.607 kb on - strand at 2332.657 kb on + strand, within PS417_10905 at 2332.657 kb on + strand, within PS417_10905 at 2332.658 kb on - strand, within PS417_10905 at 2332.688 kb on + strand, within PS417_10905 at 2332.688 kb on + strand, within PS417_10905 at 2332.688 kb on + strand, within PS417_10905 at 2332.689 kb on - strand, within PS417_10905 at 2332.845 kb on - strand at 2333.092 kb on + strand, within PS417_10910 at 2333.093 kb on - strand, within PS417_10910 at 2333.198 kb on - strand, within PS417_10910 at 2333.394 kb on + strand, within PS417_10910 at 2333.395 kb on - strand, within PS417_10910 at 2333.533 kb on + strand, within PS417_10910 at 2333.945 kb on + strand at 2333.945 kb on + strand at 2333.946 kb on - strand at 2333.946 kb on - strand at 2334.096 kb on + strand at 2334.096 kb on + strand, within PS417_10915 at 2334.097 kb on - strand, within PS417_10915 at 2334.286 kb on + strand, within PS417_10915 at 2334.450 kb on + strand, within PS417_10915 at 2334.451 kb on - strand, within PS417_10915 at 2334.451 kb on - strand at 2334.451 kb on - strand, within PS417_10915 at 2334.585 kb on - strand, within PS417_10915 at 2334.662 kb on - strand, within PS417_10915 at 2334.663 kb on + strand, within PS417_10915 at 2334.663 kb on + strand, within PS417_10915 at 2334.714 kb on + strand, within PS417_10915 at 2334.714 kb on + strand, within PS417_10915 at 2334.715 kb on - strand, within PS417_10915 at 2334.815 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x remove 2,331,863 + PS417_10900 0.64 -0.5 2,331,863 + -1.1 2,331,864 - PS417_10900 0.65 +0.5 2,332,127 + -0.1 2,332,149 - -1.0 2,332,149 - +0.5 2,332,179 - -0.7 2,332,179 - +0.9 2,332,253 + PS417_10905 0.11 +0.3 2,332,253 + PS417_10905 0.11 -0.2 2,332,254 - PS417_10905 0.11 +0.2 2,332,492 - PS417_10905 0.49 +0.4 2,332,548 + PS417_10905 0.58 +0.8 2,332,606 + PS417_10905 0.67 +0.3 2,332,606 + PS417_10905 0.67 -1.5 2,332,607 - +2.2 2,332,657 + PS417_10905 0.75 -0.7 2,332,657 + PS417_10905 0.75 -0.7 2,332,658 - PS417_10905 0.75 +0.9 2,332,688 + PS417_10905 0.80 -1.2 2,332,688 + PS417_10905 0.80 +1.2 2,332,688 + PS417_10905 0.80 -0.4 2,332,689 - PS417_10905 0.80 +0.0 2,332,845 - +0.2 2,333,092 + PS417_10910 0.25 +1.5 2,333,093 - PS417_10910 0.25 -0.1 2,333,198 - PS417_10910 0.34 -0.8 2,333,394 + PS417_10910 0.51 +1.6 2,333,395 - PS417_10910 0.51 +1.3 2,333,533 + PS417_10910 0.64 +0.2 2,333,945 + -0.5 2,333,945 + +1.2 2,333,946 - +0.0 2,333,946 - -0.1 2,334,096 + +1.2 2,334,096 + PS417_10915 0.16 +1.0 2,334,097 - PS417_10915 0.16 +0.5 2,334,286 + PS417_10915 0.36 +0.1 2,334,450 + PS417_10915 0.54 +0.1 2,334,451 - PS417_10915 0.54 -0.7 2,334,451 - +1.6 2,334,451 - PS417_10915 0.54 +0.7 2,334,585 - PS417_10915 0.68 -0.5 2,334,662 - PS417_10915 0.76 -1.5 2,334,663 + PS417_10915 0.76 -0.3 2,334,663 + PS417_10915 0.76 +0.1 2,334,714 + PS417_10915 0.82 +0.3 2,334,714 + PS417_10915 0.82 -0.8 2,334,715 - PS417_10915 0.82 +0.8 2,334,815 - +1.3
Or see this region's nucleotide sequence